Determinants of eosinophil cationic protein in nasal lavages in children

Wojnarowski, Claudia; Studnicka, Michael; Kühr, J.; Dy, Koller; Haschke, N.; Gärtner, Ch.; Renz, S; Frischer, Thomas

doi:10.1046/j.1365-2222.1998.00236.x

Cited by 13 publications

(15 citation statements)

References 28 publications

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“…Some indirect information that may point into this direction is a study performed by Wojnarowski et al [26]. They found a relatively high intra-individual variability in the concentrations of eosinophil cationic protein in nasal lavages, but not a clear circadian rhythm.…”

Section: Discussionmentioning

confidence: 96%

Variability of Exhaled Hydrogen Peroxide in Stable COPD Patients and Matched Healthy Controls

Beurden¹,

Dekhuijzen²,

Harff

et al. 2002

Respiration

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Background: Because inflammation induces oxidative stress, exhaled hydrogen peroxide (H₂O₂), which is a marker of oxidative stress, may be used as a non-invasive marker of airway inflammation in chronic obstructive pulmonary disease (COPD). There are no data on the circadian variability of exhaled H₂O₂ in COPD patients. Objective: The aim of this study was to investigate the variability of the H₂O₂ concentration in breath condensate of stable COPD patients and of matched healthy control subjects. Methods: We included 20 patients with stable mild COPD (forced expiratory volume in 1 s ∼70% of predicted) and 20 healthy subjects, matched for age, sex and pack-years, all smokers or ex-smokers. Breath condensate was collected and its H₂O₂ concentration determined fluorometrically three times on day 0 (9 and 12 a.m., and 3 p.m.) and once on days 1, 2, 3, 8 and 21. Results: The mean H₂O₂ concentration increased significantly during the day in both the patient and control groups (p = 0.02 and p < 0.01, respectively). Over a longer period up to 21 days, the mean concentration did not change in both groups. There was no significant difference between patients and controls. The mean coefficient of variation over 21 days was 45% in the patient group and 43% in the control group (p = 0.8). Conclusions: The exhaled H₂O₂ concentration increased significantly during the day in both stable COPD patients and controls. Over a period of 3 weeks, the mean H₂O₂ concentration did not change and the variability within the subjects was similar in both groups.

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Section: Discussionmentioning

confidence: 96%

Variability of Exhaled Hydrogen Peroxide in Stable COPD Patients and Matched Healthy Controls

Beurden¹,

Dekhuijzen²,

Harff

et al. 2002

Respiration

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show abstract

“…Urine was collected from each subject as a spot sample and immediately frozen at −70 °C. Before determination of EPX, urine samples were diluted 11 times in a phosphate buffer containing 0.15 mmol/L NaCl, 1% bovine serum albumine, 0.1% tween 20, 10 mmol/L EDTA, and 0.2% N‐cetyl‐trimethylammonium‐bromide, as recently described [ 16, 17]. EPX concentrations are expressed as μg/mmol creatinine.…”

Section: Methodsmentioning

confidence: 99%

“…All measurements were done in duplicate. The interassay coefficient of variation for both assays were below 11% in our laboratory [ 15, 17].…”

Section: Methodsmentioning

confidence: 99%

Assessment of eosinophil granule proteins in various body fluids: is there a relation to clinical variables in childhood asthma?

Koller¹,

Halmerbauer²,

Frischer³

et al. 1999

Clin Experimental Allergy

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Our findings demonstrate increased eosinophil activity in serum, NALF and urine derived from children with bronchial asthma. Due to the relationship between levels of eosinophil proteins in serum/urine samples and lung function, as well as significant concentration differences between symptomatic and asymptomatic asthmatic children, the assessment of eosinophil proteins in serum or urine samples appear to be more appropriate in monitoring disease activity than measurement of ECP or EPX in NALF. Thus, the determination of serum ECP/EPX or urinary EPX may be preferentially used in monitoring eosinophilic inflammation in childhood asthma.

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“…One epidemiologic study, however, has evaluated the effect of air pollution on the development of lung function in young children in Austria. 77 Repeated nasal lavage for the measurement of ECP was undertaken in 147 children over a 2-year period. Of these children, 101 underwent nasal lavage on 5 separate occasions during the first year, with an interval of a minimum of 1 week between each lavage.…”

Section: Sources Of Variancementioning

confidence: 99%

Objective monitoring of nasal airway inflammation in rhinitis

Howarth

Persson²,

Meltzer

et al. 2005

Journal of Allergy and Clinical Immunology

127

151

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Determinants of eosinophil cationic protein in nasal lavages in children

Abstract: Assessment of inflammatory mediators in nasal lavages is a useful tool for epidemiological paediatric studies. However, due to the relatively high intraindividual variability of ECP concentrations multiple lavages are necessary to characterize the individual.

Cited by 13 publications

References 28 publications

Variability of Exhaled Hydrogen Peroxide in Stable COPD Patients and Matched Healthy Controls

Variability of Exhaled Hydrogen Peroxide in Stable COPD Patients and Matched Healthy Controls

Assessment of eosinophil granule proteins in various body fluids: is there a relation to clinical variables in childhood asthma?

Objective monitoring of nasal airway inflammation in rhinitis

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