Determinants of resistance in Bacteroides fragilis strain BFR_KZ01 isolated from a patient with peritonitis in Kazakhstan

Kozhakhmetova, Saniya; Zholdybayeva, Elena; Tarlykov, Pavel; Atavliyeva, Sabina; Syzdykov, Tleuli; Daniyarov, Asset; Mukhtarova, Kymbat; Ramankulov, Yerlan

doi:10.1016/j.jgar.2021.02.022

Cited by 8 publications

(6 citation statements)

References 17 publications

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“…Ank et al (2015) isolated multidrug-resistant B.fragilis 101 from blood and B.fragilis strains detected in the gut microbiome of patients with FN. Strains O17, and O18 harbor th antibiotic resistance genes cfiA , tetQ, and nim 102 indicating possible activation of carbapenem resistance through insertion element and leading to possible treatment failure 103 .…”

Section: Discussionmentioning

confidence: 99%

Gut diversity and the resistome as biomarkers of febrile neutropenia outcome in paediatric oncology patients undergoing hematopoietic stem cell transplantation

Sardzikova,

Andrijkova,

Svec

et al. 2024

Sci Rep

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The gut microbiota of paediatric oncology patients undergoing a conditioning regimen before hematopoietic stem cell transplantation is recently considered to play role in febrile neutropenia. Disruption of commensal microbiota and evolution of opportune pathogens community carrying a plethora of antibiotic-resistance genes play crucial role. However, the impact, predictive role and association of patient´s gut resistome in the course of the therapy is still to be elucidated. We analysed gut microbiota composition and resistome of 18 paediatric oncology patients undergoing hematopoietic stem cell transplantation, including 12 patients developing febrile neutropenia, hospitalized at The Bone Marrow Transplantation Unit of the National Institute of Children´s disease in Slovak Republic and healthy individuals (n = 14). Gut microbiome of stool samples obtained in 3 time points, before hematopoietic stem cell transplantation (n = 16), one week after hematopoietic stem cell transplantation (n = 16) and four weeks after hematopoietic stem cell transplantation (n = 14) was investigated using shotgun metagenome sequencing and bioinformatical analysis. We identified significant decrease in alpha-diversity and nine antibiotic-resistance genes msr(C), dfrG, erm(T), VanHAX, erm(B), aac(6)-aph(2), aph(3)-III, ant(6)-Ia and aac(6)-Ii, one week after hematopoietic stem cell transplantation associated with febrile neutropenia. Multidrug-resistant opportune pathogens of ESKAPE, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli found in the gut carried the significant subset of patient’s resistome. Over 50% of patients treated with trimethoprim/sulfamethoxazole, piperacillin/tazobactam and amikacin carried antibiotic-resistance genes to applied treatment. The alpha diversity and the resistome of gut microbiota one week after hematopoietic stem cell transplantation is relevant predictor of febrile neutropenia outcome after hematopoietic stem cell transplantation. Furthermore, the interindividual diversity of multi-drug resistant opportunistic pathogens with variable portfolios of antibiotic-resistance genes indicates necessity of preventive, personalized approach.

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Section: Discussionmentioning

confidence: 99%

Gut diversity and the resistome as biomarkers of febrile neutropenia outcome in paediatric oncology patients undergoing hematopoietic stem cell transplantation

Sardzikova,

Andrijkova,

Svec

et al. 2024

Sci Rep

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“…Solitary colonies of contamination were collected using swabs and subsequent immersion into a tube containing Amies medium [ 98 ]. To obtain pure culture, all samples were cultivated on esculin–gelatin medium with incubation at 37 °C for 48 h under anaerobic conditions [ 99 ]. Thereafter, isolates were identified by MALDI-TOF/MS (Bruker Daltonik GmbH, Bremen, Germany), as described by Kozhakhmetova et al [ 99 ].…”

Section: Methodsmentioning

confidence: 99%

“…To obtain pure culture, all samples were cultivated on esculin–gelatin medium with incubation at 37 °C for 48 h under anaerobic conditions [ 99 ]. Thereafter, isolates were identified by MALDI-TOF/MS (Bruker Daltonik GmbH, Bremen, Germany), as described by Kozhakhmetova et al [ 99 ]. MALDI-TOF/MS scores of ≥1.7 were accepted for species assignment, and scores below 1.7 were considered unreliable [ 99 ].…”

Section: Methodsmentioning

confidence: 99%

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Effect of Plant Preservative MixtureTM on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots

Ромаданова

Толеген

Кушнаренко

et al. 2022

Plants

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Endophytic contaminants are a common problem for the in vitro propagation of woody plants and have significant economic repercussions for the conservation of plant genetic resources and commercial micropropagation. In this study, first, the microbial contamination that appeared around the base of in vitro-grown apple shoots was identified as Bacillus megaterium. Then, plant preservative mixture (PPMTM) was used as a bactericidal agent in plant tissue culture. Its efficacy for eradicating endophytic B. megaterium in in vitro cultures of apple was tested. In vitro-contaminated shoots were grown in tissue culture medium supplemented with 0.2% v/v PPMTM for 12 weeks and then transferred to medium without any PPMTM and cultured for 24 weeks. This study showed that PPMTM is an effective agent for controlling the growth of B. megaterium. Our results highlight the species-specific response of apple shoots to PPMTM. PPMTM was effective in controlling endogenous microbial contaminations from apple varieties ‘Golden Delicious’, ‘Landsberger Renette’, ‘Suislepper’, and ‘Aport krovavo-krasnyi’; meanwhile, in ‘KG 7’ and ‘Gold Rush’, all the plants grown in the absence of PPMTM were still bacterially contaminated, even though they were pre-treated for 12 weeks in PPMTM-supplemented medium. These results therefore suggest the essentiality of further testing of extended incubation of PPMTM in these cultivars that had outbreaks of bacterial contamination.

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“…Carbapenems are among the most effective drugs and are considered the drug of choice for therapy of complicated intra-abdominal infections, acute gynecological infections, and skin and soft tissue infections caused by B. fragilis. Such infections are often polybacterial and also caused by other Gram-negative bacilli [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genotypic identification of carbapenem resistance in Bacteroides fragilis clinical strains

Kierzkowska

Majewska

Karlowicz

et al. 2023

Med Microbiol Immunol

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Bacteroides fragilis is an important etiological agent of serious infections in humans. Rapid methods, readily adaptable to use in medical laboratories, are needed to detect antibiotic resistance and decrease the likelihood of therapy failure. The aim of this study was to determine the prevalence of B. fragilis cfiA-positive isolates. The second purpose was to investigate the carbapenemase activity in B. fragilis strains by Carba NP test. In the study, 5.2% of B. fragilis isolates are phenotypically resistant to meropenem. The cfiA gene was identified in 6.1% of B. fragilis isolates. The MICs of meropenem were significantly higher in cfiA-positive strains. The presence of the cfiA gene along with the IS1186 was detected in one B. fragilis strain which was resistant to meropenem (MIC 1.5 mg/L). The Carba NP test results were positive for all the cfiA-positive strains, including those susceptible to carbapenems based on their MIC values. A review of the literature revealed that the rate of B. fragilis with the cfiA gene varies from 7.6 to 38.9% worldwide. Presented results are in line with the other European studies. Phenotypic testing with the Carba NP test, it seems to be a viable alternative for the cfiA gene detection in B. fragilis isolates. The positive result obtained is of greater clinical importance than the detection of the gene cfiA.

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Determinants of resistance in Bacteroides fragilis strain BFR_KZ01 isolated from a patient with peritonitis in Kazakhstan

Cited by 8 publications

References 17 publications

Gut diversity and the resistome as biomarkers of febrile neutropenia outcome in paediatric oncology patients undergoing hematopoietic stem cell transplantation

Gut diversity and the resistome as biomarkers of febrile neutropenia outcome in paediatric oncology patients undergoing hematopoietic stem cell transplantation

Effect of Plant Preservative MixtureTM on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots

Phenotypic and genotypic identification of carbapenem resistance in Bacteroides fragilis clinical strains

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