Background: According to WHO, brucellosis is one of the 7 neglected zoonotic diseases and a major challenging issue for public health. Objectives: The aim of this study was to investigate the psychometric properties of the PRECEDE model-based scales for brucellosis prevention (PRECEDE-MSBP). Methods: In this cross-sectional study conducted in 2015, stratified random sampling was employed to recruit 420 rural residents in Ahar county, Iran. A comprehensive literature review was conducted to develop the initial draft of PRECEDE-MSBP. Face, content, and construct validity and reliability were determined. Results: Applying Exploratory Factor Analysis, the optimal solution including 37 items and 7 factors for Educational and Ecological diagnosis, 5 items and 2 factors for Environmental diagnosis, and 9 items and 3 factors for Behavioral diagnosis were emerged, which accounted for 60.43%, 56.51%, and 53.75% of the total variances, respectively. Appropriate validity, reliability, functionality, and simplicity were demonstrated for PRECEDE-MSBP. Conclusions: Researchers and health care providers interested in brucellosis prevention may apply the PRECEDE-MSBP inventory as a suitable instrument to provide the best information while conducting brucellosis prevention need assessments for their interventional efforts. However, further studies applying this instrument are needed in order to compare the different aspects of PRECEDE-MSBP in different communities.