Determination and Confirmation of Narasin and Monensin in Chicken, Swine, and Bovine Tissues by LC/MS/MS: Final Action 2011.24

Lombardi, Kimberly R; Burnett, Thomas J; Brunelle, Sharon L; Ulrey, W Dennis; Coleman, Mark R

doi:10.5740/jaoacint.12-394

Cited by 5 publications

(4 citation statements)

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“…However, it was determined that a significant portion of the analytes were not extracted at the initial step of the method, some were not extracted at all. Various proportions of iso‐octane to ethyl acetate were evaluated, but to no avail . Extraction processes based on QuEChERS ( Qu ick, E asy, Ch eap, E ffective, R ugged, and Safe) were available, but required a dispersive SPE step .…”

Section: Discussionmentioning

confidence: 99%

“…Various proportions of iso-octane to ethyl acetate were evaluated, but to no avail. [3] Extraction processes based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) were available, but required a dispersive SPE step. [4] Methods using acetonitrile where the crude extract was evaporated, re-dissolved, and injected into the mass spectrometry system were found not to be optimum for liver tissues.…”

Section: Evaluation Of Existing Methodsmentioning

confidence: 99%

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A multi‐residue method for 17 anticoccidial drugs and ractopamine in animal tissues by liquid chromatography‐tandem mass spectrometry and time‐of‐flight mass spectrometry

Matus

Boison

2016

Drug Testing and Analysis

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A new and sensitive multi-residue liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatographyquadrupole time-of-flight-mass spectrometry (LC-QToF-MS) method was developed and validated for the determination and confirmation of residues of 17 anticoccidials, plus free ractopamine in poultry muscle and liver, and bovine muscle, liver, and kidney tissues. The 17 anticoccidials are lasalocid, halofuginone, narasin, monensin, semduramicin, ethopabate, robenidine, buquinolate, toltrazuril as its sulfone metabolite, maduramicin, salinomycin, diclazuril, amprolium, decoquinate, dinitolmide, clopidol, and the nicarbazin metabolite DNC (N,N1-bis(4-nitrophenyl)urea). The analytes were extracted and cleaned up within a 3-hour period by simply extracting the analytes into a solvent mixture with salts followed by centrifugation, dilution, and filtration. The validated method was used in a pilot study for the analysis of 173 samples that included quail liver, bovine kidney, liver, muscle, and horse muscle. The predominant residues found in this study were monensin, ractopamine, and lasalocid. The results of this pilot study showed that this new method is applicable to real samples, and is fit for use in a regulatory testing programme.

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Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Existing Methodsmentioning

confidence: 99%

A multi‐residue method for 17 anticoccidial drugs and ractopamine in animal tissues by liquid chromatography‐tandem mass spectrometry and time‐of‐flight mass spectrometry

Matus

Boison

2016

Drug Testing and Analysis

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“…So far, the determination of polyether ionophore antibiotic and s‐triazine antiparasitic residues mostly focus on eggs , poultry tissues , milk , and feeds . There are also some LC–MS/MS methods focus only on one or two coccidiostats . So far, there are few methods being reported which cover all of the residual target tissue such as muscle, fat, liver, kidney, and animal origin products (milk, egg) for these two kinds of drugs.…”

Section: Introductionmentioning

confidence: 99%

Magnetic solid‐phase extraction based on carbon nanotubes for the determination of polyether antibiotic and s‐triazine drug residues in animal food with LC–MS/MS

Liu

Xie

et al. 2017

J of Separation Science

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Carbon nanotubes-magnetic nanoparticles, comprising ferroferric oxide nanoparticles and carbon nanotubes, were prepared through a simple one-step synthesis method and subsequently applied to magnetic solid-phase extraction for the determination of polyether antibiotic and s-triazine drug residues in animal food coupled with liquid chromatography with tandem mass spectrometry. The nanocomposites were characterized by transmission electron microscopy, X-ray diffraction, and vibrating sample magnetometry. The components within the nanocomposites endowed the material with high extraction performance and manipulative convenience. Compared with carbon nanotubes, the as-prepared carbon nanotubes-magnetic nanoparticles showed better extraction and separation efficiencies for polyether antibiotics and s-triazine drugs thanks to the contribution of the iron-containing magnetic nanoparticles. Various experimental parameters affecting the extraction efficiency had been investigated in detail. Under the optimal conditions, the good linearity ranging from 1 to 200 μg/kg for diclazuril, toltrazuril, toltrazuril sulfone, lasalocid, monensin, salinomycin, narasin, nanchangmycin, and maduramicin, low limits of detection ranging from 1 to 5 μg/kg, and satisfactory spiked recoveries (77.1-91.2%, with the inter relative standard deviation values from 4.0 to 12.2%) were shown. It was confirmed that this novel method was an efficient pretreatment and enrichment procedure and could be successfully applied for extraction and determination of polyether and s-triazine drug residues in complex matrices.

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“…It may accumulate in tissues of edible animals [8,9]. It is effective by permeabilizing cell membranes thus dissipating ion gradients of susceptible bacteria [6].…”

Section: Introductionmentioning

confidence: 99%

Stimulation of Eryptosis by Narasin

Bouguerra

Bissinger

Abbès

et al. 2015

Cell Physiol Biochem

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Background/Aims: Narasin, an ionophore used for the treatment of coccidiosis, has been shown to foster apoptosis of tumor cells. In analogy to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by Ca²⁺ entry with subsequent increase of cytosolic Ca²⁺ activity ([Ca²⁺]_i), and by ceramide. The present study explored, whether and how narasin induces eryptosis. Methods: Flow cytometry was employed to estimate phosphatidylserine exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca²⁺]_i from Fluo3-fluorescence, and ceramide abundance utilizing specific antibodies. Results: A 48 hours exposure of human erythrocytes to narasin (10 and 25 ng/ml) significantly increased the percentage of annexin-V-binding cells. Forward scatter was decreased by 1 ng/ml narasin but not by higher narasin concentrations (10 and 25 ng/ml). Narasin significantly increased Fluo3-fluorescence (10 and 25 ng/ml) and slightly, but significantly increased ceramide abundance (25 ng/ml). The effect of narasin on annexin-V-binding was significantly blunted, but not abolished by removal of extracellular Ca²⁺. Conclusions: Narasin triggers phospholipid scrambling of the erythrocyte cell membrane, an effect paralleled and partially dependent on Ca²⁺ entry. Narasin further leads to cell shrinkage and slight increase of ceramide abundance.

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Determination and Confirmation of Narasin and Monensin in Chicken, Swine, and Bovine Tissues by LC/MS/MS: Final Action 2011.24

Cited by 5 publications

References 0 publications

A multi‐residue method for 17 anticoccidial drugs and ractopamine in animal tissues by liquid chromatography‐tandem mass spectrometry and time‐of‐flight mass spectrometry

A multi‐residue method for 17 anticoccidial drugs and ractopamine in animal tissues by liquid chromatography‐tandem mass spectrometry and time‐of‐flight mass spectrometry

Magnetic solid‐phase extraction based on carbon nanotubes for the determination of polyether antibiotic and s‐triazine drug residues in animal food with LC–MS/MS

Stimulation of Eryptosis by Narasin

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