Determination and Distribution Study of Myrislignan in Rat Tissues by RP-HPLC

Wang, Ying; Liu, Jiaxi; Zhang, You-Bo; Li, Fēi; Yang, Xiu‐Wei

doi:10.1007/s10337-012-2219-3

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…In addition, myrislignan could be well transported in the Caco-2 cell monolayer model (Yang, Huang, Ma, Wu, & Xu, 2010). It is also distributed to different tissues after intravenous administration to rats (Wang, Liu, Zhang, Li, & Yang, 2012). The current study also indicated that myrislignan is a potent inhibitor of NO production.…”

Section: Resultsmentioning

confidence: 99%

New neolignans from the seeds of Myristica fragrans that inhibit nitric oxide production

Cao

Yang

et al. 2015

Food Chemistry

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Five new 8-O-4' type neolignans, named myrifralignan A-E (1-5), together with five known analogues (6-10), were isolated from the seeds of Myristica fragrans Houtt. Their chemical structures were determined using several spectroscopic methods. Compounds 3-10 exhibited potent inhibitory activity against the production of nitric oxide (NO) in the RAW264.7 cell line stimulated by lipopolysaccaride. Myrislignan (7) and machilin D (10) were the most potent inhibitors of NO production amongst these compounds. The IC50 values of myrislignan and machilin D were 21.2 and 18.5 μM. And, their inhibitory activity was more than L-N(6)-(1-iminoethyl)-lysine, a selective inhibitor of inducible nitric oxide synthase (IC50=27.1 μM). Furthermore, real-time PCR analysis revealed that these neolignans could significantly suppress the expression of inducible nitric oxide synthase mRNA. These results demonstrated that the 8-O-4' type neolignans are promising candidates as anti-inflammatory agents.

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Section: Resultsmentioning

confidence: 99%

New neolignans from the seeds of Myristica fragrans that inhibit nitric oxide production

Cao

Yang

et al. 2015

Food Chemistry

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“…While the exact mechanism of action by which nutmeg exerts its effects on pathogenic bacteria has not been completely elucidated, several antibacterial constituents isolated from nutmeg have been identified, such as macelignan and trimyristin . Tissue distribution studies also revealed that myrislignan and dehydrodiisoeugenol, two representative constituents in nutmeg, are higher in concentration in the intestine than in other tissues, , suggesting that high levels of nutmeg in the intestine contribute to inhibiting the growth of pathogenic bacteria. These results demonstrate that the gut microbiota and its metabolism regulated by nutmeg may be of therapeutic value against gastrointestinal diseases, including colon cancer and colitis.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Colon Cancer by Nutmeg

Yang

Krausz

et al. 2015

J. Proteome Res.

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Colon cancer is the most common cancer and the third leading cause of cancer mortality in humans. Using mass spectrometry-based metabolomics, the current study revealed the accumulation of four uremic toxins (cresol sulfate, cresol glucuronide, indoxyl sulfate, and phenyl sulfate) in the serum of mice harboring adenomatous polyposis coli (APC) gene mutation-induced colon cancer. These uremic toxins, likely generated from the gut microbiota, were associated with an increase in the expression of the proinflammatory cytokine IL-6 and a disorder of lipid metabolism. Nutmeg, which exhibits antimicrobial activity, attenuated the levels of uremic toxins and decreased intestinal tumorigenesis in Apc(min/+) mice. Nutmeg-treated Apc(min/+) mice had decreased IL-6 levels and normalized dysregulated lipid metabolism, suggesting that uremic toxins are responsible, in part, for the metabolic disorders that occur during tumorigenesis. These studies demonstrate a potential biochemical link among gut microbial metabolism, inflammation, and metabolic disorders and suggest that modulation of gut microbiota and lipid metabolism using dietary intervention or drugs may be effective in colon cancer chemoprevention strategies.

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The action and mechanism of myrislignan on A549 cells in vitro and in vivo

Yang²,

Chen

et al. 2016

J Nat Med

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Myrislignan is a natural compound with little pharmacological study. In our investigation, we investigated the effect of myrislignan in the induction of apoptosis in A549 cells in vitro and in vivo. Myrislignan inhibited the proliferation of A549 cells in a dose- and time-dependent manner assayed by MTT. In addition, Hoechst flow cytometry showed that myrislignan significantly induced apoptosis and cell cycle arrest in A549 cells. The apoptosis and anti-cell proliferation was mediated by the activation of mitogen-activated protein kinase and the inhibition of epidermal growth factor receptor signal pathway, change of mitochondrial membrane potential, the releasing of c-Myc, the downregulation of the level of the anti-apoptotic protein Bcl-2, and the upregulation of the level of the pro-apoptotic protein Bax. In conclusion, those results reveal a potential mechanism for the anti-cancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a promising compound for the treatment of lung cancer.

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Determination and Distribution Study of Myrislignan in Rat Tissues by RP-HPLC

Cited by 7 publications

References 17 publications

New neolignans from the seeds of Myristica fragrans that inhibit nitric oxide production

New neolignans from the seeds of Myristica fragrans that inhibit nitric oxide production

Modulation of Colon Cancer by Nutmeg

The action and mechanism of myrislignan on A549 cells in vitro and in vivo

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