Determination and Evaluation of Doses of Metronidazole in Different Quantities and Formulations with Multiple Spectroscopic Methods

doi:10.31838/srp.2020.5.21

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…The principle of the drug determination in the samples lies in the initial reduction in MTZ as an imidazole derivative by treatment with zinc powder and hydrochloric acid followed by diazotization and coupling of the resulting amine [32]. It means that the selected and filtrated samples were adjusted before spectrophotometric determination of drug concentration as follows: 2 mL of filtrate was reduced by 10 mg of powdered zinc, 1 mL of hydrochloric acid (1M) was added and the mixture was shaken, 1 mL of sodium nitrite solution (1%) was added and the mixture was allowed to stand for 5 min, then 1 mL of sulphamic acid solution (3%) was added with occasional shaking for 3 min.…”

Section: In Vitro Drug Releasementioning

confidence: 99%

Mucoadhesive Vaginal Tablets Containing Metronidazole: Screening of Optimal Natural Polymer in the Composition

Bartoníková,

Špaglová,

Papadakos

et al. 2024

Sci. Pharm.

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(1) Background: The study aimed to compare the impact of various natural polymers–sodium alginate, acacia gum, carrageenan, guar gum, xanthan gum, and tragacanth on the formulation and the physical properties of mucoadhesive vaginal tablets containing metronidazole (167 mg/g). (2) Methods: The quality of the tablets prepared by direct compression was evaluated by pharmacopoeia tests (uniformity of mass, resistance to crushing, friability). Mucoadhesion of the tablets was characterized by swelling capacity and mucoadhesive strength, i.e., the force required to detach the tablet from the rabbit mucosa. In vitro drug release was performed by a modified dissolution method in paddle apparatus containing the simulated vaginal fluid (pH 4.5). Scanning electron microscopy observed morphological changes on the swollen tablets’ surface. (3) Results: Pharmacopoeia tests have shown that all prepared tablets met the requirements on quality. The highest mucoadhesive strength was noted in tablets containing guar and xanthan gum. The highest swelling capacity was possessed by tablets containing carrageenan. (4) Conclusions: Summarizing all tests’ results, sodium alginate can be considered the most suitable natural polymer in tablet formulation. The combination of polymers providing higher mucoadhesiveness and at the same time a prolonged release, e.g., xanthan or guar, together with sodium alginate, could also be of interest.

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Section: In Vitro Drug Releasementioning

confidence: 99%

Mucoadhesive Vaginal Tablets Containing Metronidazole: Screening of Optimal Natural Polymer in the Composition

Bartoníková,

Špaglová,

Papadakos

et al. 2024

Sci. Pharm.

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“…All additions were stored at a cold temperature. This is due to diazonium salt's poor thermal stability, which requires low temperatures at 0-5C [18]. The mechanism of diazonium salt formation is shown in Figure 4.…”

Section: Formation An Azo Dyementioning

confidence: 99%

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Almira¹

2022

ijcsrr

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This research aimed to determine the concentration of chloramphenicol in shrimp using the standard addition method based on the diazotization reaction using Zn powder as a reducing agent of chloramphenicol, followed by the use of N-(1-naphthyl) ethylenediamine dihydrochloride as a coupling agent and measured at 565 nm. Based on the test, the shrimp sample was found to contain 1964.91 mg/kg of chloramphenicol and it exceeded the requirements set by the European Commission which was 0,15 mg/kg. The limit of detection (LOD) value is 0.19 mg/mL and the limit of quantitation (LOQ) value is 0.64 mg/mL. The correlation coefficient (R2) was 0.9991 for the concentration range of 0-50 ppm. The analysis of the results showed that the %recovery in shrimp analysis using this method was 87.41%-107.73% with an average of 109.38%.

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Novel Rapid Validated TLC- Densitometry for the Simultaneous Determination of Three Co-formulated Drugs used for Deep Tooth Inflammation Treatment

Marouf

Kawas

Sakur

2021

IRJPAC

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A new use of a tertiary antibiotic combination of ciprofloxacin, clindamycin and metronidazole is currently being studied as an intracranial (intracranial) drug in an effort to disinfect the root canal system to revitalize the tooth with dead pulp. It is necessary to developing analytical method for the new drug combination. Herein we developed a rapid validated thin-layer chromatography (TLC)‒densitometric method has been developed for the simultaneous determination of 3 co-formulated drugs used for deep tooth inflammation Treatment. The studied drugs are Ciprofloxacin.HCL (CIP), Clindamycin phosphate (CLI) and Metronidazole(MET). The separation was achieved by using silica gel 60 F254 plates (20*20 cm) as stationary Phase and the developing system of Dichloromethane:n-Hexane:methanol:Ethylamine:Triethylamine (40:20:32:3:5)v/v. Densitometry scanning was performed at 220 nm. The Rf values of CIP, CLI and MET were found to be 0.588, 0.776 and 0.898 respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines and was successfully applied for the analysis of ternary lab made mixture, containing the cited ternary mixture without interference from excipients. There is no previously published TLC–densitometry method for the determination of the previously mentioned ternary mixture. The suggested method is novel, rapid, accurate, reproducible and of low cost, so; thus, it can be used for quality control analysis of these formulations.

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Determination and Evaluation of Doses of Metronidazole in Different Quantities and Formulations with Multiple Spectroscopic Methods

Cited by 3 publications

References 18 publications

Mucoadhesive Vaginal Tablets Containing Metronidazole: Screening of Optimal Natural Polymer in the Composition

Mucoadhesive Vaginal Tablets Containing Metronidazole: Screening of Optimal Natural Polymer in the Composition

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

Novel Rapid Validated TLC- Densitometry for the Simultaneous Determination of Three Co-formulated Drugs used for Deep Tooth Inflammation Treatment

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