Determination and Nature of Leaf Sterols

Wall, Monroe E.; Kelley, Edward G.

doi:10.1021/ac60009a021

Cited by 19 publications

(13 citation statements)

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“…Sterols can be quantified by the colorimetric Liebermann-Burchard reaction (Wall, Kelley, 1947;Sabir et al, 2005), which is positive with stenols and negative with stanols (Brooks, 1970).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of alginate-starch-chitosan microparticles containing stigmasterol through the external ionic gelation technique

Fujiwara

Campos

Costa

et al. 2013

Braz. J. Pharm. Sci.

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Stigmasterol -a plant sterol with several pharmacological activities -is susceptible to oxidation when exposed to air, a process enhanced by heat and humidity. In this context, microencapsulation is a way of preventing oxidation, allowing stigmasterol to be incorporated into various pharmaceutical forms while increasing its absorption. Microparticles were obtained using a blend of polymers of sodium alginate, starch and chitosan as the coating material through a one-stage process using the external gelation technique. Resultant microparticles were spherical, averaging 1.4 mm in size. Encapsulation efficiency was 90.42% and method yield 94.87%. The amount of stigmasterol in the oil recovered from microparticles was 9.97 mg/g. This technique proved feasible for the microencapsulation of stigmasterol.Uniterms: Stigmasterol/ microencapsulation. Microencapsulation. Sodium alginate/drugs coating. Chitosan/drugs coating. Starch/drugs coating. Natural polymers/drugs coating.O estigmasterol, um fitoesterol com diversas atividades farmacológicas, é suscetível à oxidação quando exposto ao ar, calor e umidade. Neste contexto, a microencapsulação é uma forma de proteção contra oxidação, permitindo a incorporação do estigmasterol em diversas formas farmacêuticas e aumentar sua absorção. As micropartículas foram obtidas por gelificação iônica externa, em uma etapa, utilizando como revestimento polímeros naturais de alginato de sódio, amido de milho e quitosana. As micropartículas apresentaram formato esférico com tamanho aproximado de 1,4 mm. O rendimento foi de 94,87% e a eficiência média de encapsulação de 90,42%. A quantidade de estigmasterol no óleo recuperado das micropartículas foi de 9,97 mg/g. O método mostrou-se viável para a microencapsulação do estigmasterol.Unitermos: Estigmasterol/microencapsulação. Microencapsulamento. Alginato de sódio/revestimento de fámacos. Quitosana/revestimento de fámacos. Amido/revestimento de fámacos. Polímeros naturais/ revestimento de fámacos.

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“…Sterols can be quantified by the colorimetric Liebermann-Burchard reaction (Wall, Kelley, 1947;Sabir et al, 2005), which is positive with stenols and negative with stanols (Brooks, 1970).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of alginate-starch-chitosan microparticles containing stigmasterol through the external ionic gelation technique

Fujiwara

Campos

Costa

et al. 2013

Braz. J. Pharm. Sci.

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“…Free sterols in non-saponifiable fractions were extracted with petroleum ether and were then precipitated with digitonin to form sterol digitonides (7). The sterol content was obtained by multiplying the weight of digitonides by a factor of 0.253 (8).…”

Section: Methodsmentioning

confidence: 99%

Sterol Content in Cigarette Tobacco and Smoke

Cheng¹

1973

Beiträge Zur Tabakforschung / Contributions to Tobacco Research

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“…Aliquots of this solution were subjected to the Liebermann-Burchard reaction, and the intensity of the color produced was measured with a Beckman spectrophotometer. The digitonide of alfalfa sterol has an absorption maximum at 680 mu (5). Absorption maxima for the digitonides of the sterols of brome grass and the wheat plant were found at 680 mu also ( fig.…”

mentioning

confidence: 94%

“…This perhaps has been due partially to the difficulty of determining sterols quantitatively in the presence of chlorophyll and carotenoids. With an increase in the use of certain sterols for the production of pharmaceuticals, attention has been directed again to the sterols of green plant tissue, with methods being developed to facilitate their estimation (5). The study reported herein was undertaken to determine the nature and the amount of the sterols in the lipid fraction of some crops which are suitable for commercial dehydration, for such dehydrated mat-erial would be the logical source for the production of sterols from green plants if they are found to be economically important.…”

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confidence: 99%

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The Sterols of Alfalfa and Certain Cereal Grasses

Blair¹,

Mitchell²,

Silker³

1951

Plant Physiol.

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Little attention has been given to the sterols of the vegetative portion of green plants. This perhaps has been due partially to the difficulty of determining sterols quantitatively in the presence of chlorophyll and carotenoids. With an increase in the use of certain sterols for the production of pharmaceuticals, attention has been directed again to the sterols of green plant tissue, with methods being developed to facilitate their estimation (5). The study reported herein was undertaken to determine the nature and the amount of the sterols in the lipid fraction of some crops which are suitable for commercial dehydration, for such dehydrated mat-erial would be the logical source for the production of sterols from green plants if they are found to be economically important.Determination and characterization of sterols Fresh plant tissue was autoclaved for five minutes at 10 pounds pressure, and dried at 650 C for four hours. The dried material was ground to pass through a 20-mesh screen. Sterols were determined quantitatively by the colorimetric method of WALL and KELLEY (5), which consists of extracting the dried plant tissue with Skellysolve B, removing chlorophyll and xanthophyll by adsorption, precipitating carotene with iodine, precipitating the sterols with digitonin, and subjecting the resulting sterol digitonide to the Liebermann-Burchard reaction. A standard curve was established for each plant tissue to be studied by isolating the sterol with digitonin (5) and dissolving a weighed amount of the sterol digitonide in acetic acid. Aliquots of this solution were subjected to the Liebermann-Burchard reaction, and the intensity of the color produced was measured with a Beckman spectrophotometer. The digitonide of alfalfa sterol has an absorption maximum at 680 mu (5). Absorption maxima for the digitonides of the sterols of brome grass and the wheat plant were found at 680 mu also (fig.

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Determination and Nature of Leaf Sterols

Cited by 19 publications

References 10 publications

Production and characterization of alginate-starch-chitosan microparticles containing stigmasterol through the external ionic gelation technique

Production and characterization of alginate-starch-chitosan microparticles containing stigmasterol through the external ionic gelation technique

Sterol Content in Cigarette Tobacco and Smoke

The Sterols of Alfalfa and Certain Cereal Grasses

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