In the semimicro method for nitrogen, the plant sample is digested with C11SO4 or HgO as catalyst in an acid medium, EDTA is added to prevent interference from metals, and the res ulting solution is neutralized to pH 7. Sod ium nitroprusside is added and the color is allowed to develop 1hr ; the absorbance is measured at 625 n m . The proposed method, with either CUSO4 or HgO, is applicable to dry or fresh plant tissues and yields results which are comparable to those obtained with AOAC 2.049-2.051.

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The authors wish to acknowledge the assistance of Lowell Jordan and Robert Schieferstien in the determinations of CMU. and to thank S. C. Fang for his helpful suggestions and D. E. Bullís for review of the paper. Literature Cited(1) Assoc. Offic. Agr. Chemists, "Official Methods of Analysis," 7th edition.(3.39a) 1950. (3.3) 1950.(2) Bouvoucos, G. J.. J. Am. Soc. Agron., 22, 747-51 (1930).

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