1972
DOI: 10.1093/jaoac/55.1.1
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Micro determination of Nitrogen in Plant Tissues

Abstract: In the semimicro method for nitrogen, the plant sample is digested with C11SO4 or HgO as catalyst in an acid medium, EDTA is added to prevent interference from metals, and the res ulting solution is neutralized to pH 7. Sod ium nitroprusside is added and the color is allowed to develop 1hr ; the absorbance is measured at 625 n m . The proposed method, with either CUSO4 or HgO, is applicable to dry or fresh plant tissues and yields results which are comparable to those obtained with AOAC 2.049-2.051.

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Cited by 21 publications
(18 citation statements)
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“…For the proximate analysis, moisture was determined using the method described by Lovell (1974); crude protein by the semi-micro kjeldahl digestion and distillation (Mitchell 1972); crude fat by the soxhlet ether extraction method (Maynard 1970); crude fibre by successive digestion with dilute acid and alkali (Association of Official Agricultural Chemists 1945); ash by using an ashing furnace (Maynard 1970); and calcium and phosphorus as described by Lovell (1974); nitrogen free extract (NFE) was computed by taking the sum of values for crude protein, crude fat, ash and crude fibre and subtracting this from 100 (Maynard et al 1979).…”
Section: Experimental Designsupporting
confidence: 87%
“…For the proximate analysis, moisture was determined using the method described by Lovell (1974); crude protein by the semi-micro kjeldahl digestion and distillation (Mitchell 1972); crude fat by the soxhlet ether extraction method (Maynard 1970); crude fibre by successive digestion with dilute acid and alkali (Association of Official Agricultural Chemists 1945); ash by using an ashing furnace (Maynard 1970); and calcium and phosphorus as described by Lovell (1974); nitrogen free extract (NFE) was computed by taking the sum of values for crude protein, crude fat, ash and crude fibre and subtracting this from 100 (Maynard et al 1979).…”
Section: Experimental Designsupporting
confidence: 87%
“…For analysis of nitrogen, phosphorus, potassium, and sulfur, the samples were dried in a forced air oven at 60 °C (140.0 ºF) and ground through a 40-mesh screen before wet digestion. The digestion for nitrogen analysis was as described by Mitchell (1972). A nitric acid digestion method was used to prepare the leaf samples for potassium, phosphorus, and sulfur analysis (Perkin-Elmer, 1982).…”
Section: Research Reportsmentioning
confidence: 99%
“…A nitric acid digestion method was used to prepare the leaf samples for potassium, phosphorus, and sulfur analysis (Perkin-Elmer, 1982). Nitrogen, phosphorus, and sulfur were analyzed colorimetrically (Mitchell, 1972;Jackson, 1958); and potassium was determined using atomic absorption spectrometry at 766.5 nm (model 3100; Perkin-Elmer Analytical Instruments, Shelton, Conn.).…”
Section: Research Reportsmentioning
confidence: 99%
“…Ethylene pro-duction was then measured using a Hewlett Packard 5890 Series II gas chromatograph. A second method for assessing symbiotic effectiveness involved measurement of shoot nitrogen content for plants inoculated with different bacterial isolates using the method of Mitchell (1972). Finally, biomass gain by inoculated plants relative to uninoculated control plants was measured.…”
Section: Symbiotic Effectivenessmentioning
confidence: 99%