Determination and separation of putrescine and spermidine in aquatic products

Abstract

As shown in the figure, the BAs were well separated within 10 min. According to the standard migration time of putrescine and spermidine, the first peak in this diagram represents the existence of putrescine, while spermidine is not detected or the concentration is lower than the detection limit of this method.

Comparison of Mobile Phone and CCD Cameras for Electrochemiluminescent Detection of Biogenic Amines

“…The subsequent experiments demonstrate the optimization of the system and determination of analytical figures of merit. Prior work in our lab and literature searches [23][24][25][26][27][28]55] indicated that a Ru(bpy) 3 2+ concentration of ~4-5 mM would lead to the highest signal-to-background ratio. Prior literature reports have demonstrated that the ECL reaction between Ru(bpy) 3 2+ and amines provides the highest signal at neutral to slightly basic pH values [32][33][34][35]55].…”

“…Prior literature reports have demonstrated that the ECL reaction between Ru(bpy) 3 2+ and amines provides the highest signal at neutral to slightly basic pH values [32][33][34][35]55]. For work with biogenic amines, the general consensus is that pH 7-9 leads to the highest S/N, but pH 9 can be preferable for primary amines such as putrescine and histamine [23][24][25][26][27][28]. Therefore, in the next section, ECL work was performed at the optimal pH for each amine.…”

“…The electrochemical method, electrogenerated chemiluminescence (ECL, or electrochemiluminescence), has been coupled with capillary electrophoresis (CE) [23][24][25][26][27] and used on its own [28,29] to detect various biogenic amines. ECL [30][31][32][33][34][35][36] combines the qualities of electrochemical methods (inexpensive, easy to miniaturize) with the sensitivity of luminescent measurements.…”

“…ECL also provides some inherent selectivity to an analytical method, as the species being detected both must oxidize under the potential applied and participate in the ECL reaction with the luminescent species. BA have been shown to participate in the oxidative-reductive ECL reaction with tris(2,2 -bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ , Figure S1) [23][24][25][26][27][28][29]. An ECL mechanism between Ru(bpy) 3 2+ and aminecontaining species is shown in Figure S2 [37,38].…”