Determination and uptake of abamectin and difenoconazole in the stingless bee Melipona scutellaris Latreille, 1811 via oral and topic acute exposure

Prado, Fernanda Scavassa Ribeiro do; Santos, Dayana Moscardi dos; Oliveira, Thiessa Maramaldo de Almeida; Burgarelli, José Augusto Micheletti; Castele, Janete Brigante; Vieira, Eny María

doi:10.1016/j.envpol.2020.114313

Cited by 17 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No macrocyclic lactone was detected on any wildflower collected at reference sampling locations. Although abamectin is applied to crops via foliar spray, most macrocyclic lactones are used as anthelmintics in livestock (do Prado et al, 2020;Prichard et al, 2012). Macrocyclic lactones are administered to cattle via ear tags, injections, pour-ons, and orally to combat internal and external pests (Feedlot, 2011;Peterson et al, 2020;Prichard et al, 2012;Urech, 2004).…”

Section: Agrochemicals On Wildflowersmentioning

confidence: 99%

Agrochemical occurrence on colocated wildflowers and wild bees collected near beef cattle feed yards and row crops

Peterson

Green

Subbiah

et al. 2021

Integr Envir Assess & Manag

View full text Add to dashboard Cite

It is well established that agrochemicals can pose significant threats to native pollinators; however, relatively little is known about pollinator risks associated with agrochemicals that are used on beef cattle feed yards. Recently, feed yard-derived agrochemicals and those from row crop agriculture were quantified on wildflowers growing on the High Plains, USA. To better characterize pollinator risks on the High Plains, we collected colocated wildflowers and foraging bees across three field seasons for analytical determination of residual agrochemicals. Agrochemicals were detected and quantified on the majority of wildflowers (85%) and nearly half of bees (49%). Permethrin was the most frequently detected analyte on wildflowers (32%) and bees (17%). Flower hazard quotients and flower hazard indices were calculated to deterministically evaluate risk to foraging pollinators. Mean flower hazard quotients exceeded one for 5/16 analytes (31%), and flower hazard quotients calculated for 30% of wildflowers were greater than 50. Flower hazard quotients for clothianidin exceeded 400 for 14% of wildflowers, which portends conditions conducive to frequent bee mortalities. Flower hazard indices were greater on wildflowers from mid-July to mid-September as compared with wildflowers collected earlier in the summer, which coincides with row crop planting and increased prevalence of feed yard flies. Hazard quotients and hazard index values calculated from agrochemical residue data suggest that pollinators frequenting wildflowers near beef cattle feed yards and row crops on the High Plains are at risk from both individual sources, and more so when considered in combination.

show abstract

Section: Agrochemicals On Wildflowersmentioning

confidence: 99%

Agrochemical occurrence on colocated wildflowers and wild bees collected near beef cattle feed yards and row crops

Peterson

Green

Subbiah

et al. 2021

Integr Envir Assess & Manag

View full text Add to dashboard Cite

show abstract

“…Another biomolecule with potential insecticidal properties called Abamectin is a metabolite of Streptomyces avermitilis, belonging to the Avermectin class. This biochemical compound is extremely toxic to humans (0.002 mg/kg) and other vertebrates as well as invertebrates (Prado et al 2020). A study of abamectin verified its bioaccumulation in tissues of adult forager bees of Melipona scutellaris after oral and topical exposure, and mortality was higher from oral than topical exposure (Table III) These studies challenge the common perception of non-target safety of these bioinsecticides, particularly for stingless bee species (Table III).…”

Section: Biopesticidesmentioning

confidence: 91%

The stingless bees (Hymenoptera: Apidae: Meliponini): a review of the current threats to their survival

et al. 2022

View full text Add to dashboard Cite

Pollination by insects is an essential service for the conservation of plant biodiversity, since it increases the rate of fertilization of flowers and therefore increases the quality and quantity of seeds and fruits. In tropical regions, native plants depend heavily on stingless bees as pollinators since before the introduction of the European honey bee Apis mellifera. However, like for many other insect species worldwide, anthropogenic actions represent a true threat to stingless bee populations. In this article, we review the works that evaluated the potential negative impacts of human activities on populations of stingless bees. The studies constitute a strong body of evidence that stingless bee populations are at risk of decline around the world due to threats including habitat loss, agrochemicals, competition for resources, climate change, and the introduction of exotic species, including pathogens.

show abstract

“…In the acute topical tests, exposure was performed by the direct application of 1 μl of an aqueous solution containing different concentrations of each insecticide in a Triton X-100 (1.0%) solution: 1 μg ml −1 (Topical A) and 2.5 μg ml −1 (Topical B; do Prado et al, 2020). The solutions were applied with a microapplicator to the dorsal side of the thorax of each bee.…”

Section: Oral and Topical Experimentsmentioning

confidence: 99%

Modified QuEChERS Method for Extracting Thiamethoxam and Imidacloprid from Stingless Bees: Development, Application, and Green Metrics

Rahman

Ali

Xavier

et al. 2022

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

In the present study, a method for the determination of residues of the neonicotinoid insecticides thiamethoxam and imidacloprid in the stingless bee Melipona scutellaris Latreille (1811) was optimized through a factorial design, tested using green metrics, and then applied to exposed bees. It combines the extraction with a modified quick, easy, cheap, effective, rugged, and safe method and the determination by liquid chromatography–tandem mass spectrometry analysis. Different parameters such as the mass of the sample, dispersive sorbents, and elution solvents were assessed. Method validation parameters were checked and include sensitivity, specificity, and linearity. The limit of quantification of 0.0025 μg g–1 was obtained for both insecticides, where accuracy was 94%–100% with satisfactory intraday and interday precisions (relative standard deviation <10%). The qualified method was applied to orally and topically exposed bee samples, and the results indicated that it is suitable for the determination and quantification of neonicotinoid pesticide residues in this species. Moreover, green analytical metrics like the National Environmental Methods Index, Eco Scale score, high‐performance liquid chromatography with an environmental assessment tool (HPLC‐EAT), waste generation, and amount of sample were compared with methods described in the literature involving neonicotinoid analysis in honeybees. As a result, the present study displayed the highest Eco Scale score and HPLC‐EAT score and the second smallest amount of sample and waste generated. Thus, the method meets green analytical metrics more than other methods. In this sense, besides the application, the multicriteria decision analysis tool employed suggests that this is a good option as a green analytical method. Environ Toxicol Chem 2022;41:2365–2374. © 2022 SETAC

show abstract

Determination and uptake of abamectin and difenoconazole in the stingless bee Melipona scutellaris Latreille, 1811 via oral and topic acute exposure

Cited by 17 publications

References 46 publications

Agrochemical occurrence on colocated wildflowers and wild bees collected near beef cattle feed yards and row crops

Agrochemical occurrence on colocated wildflowers and wild bees collected near beef cattle feed yards and row crops

The stingless bees (Hymenoptera: Apidae: Meliponini): a review of the current threats to their survival

Modified QuEChERS Method for Extracting Thiamethoxam and Imidacloprid from Stingless Bees: Development, Application, and Green Metrics

Contact Info

Product

Resources

About