Determination of 1,25-dihydroxyvitamin D2 in rat serum using liquid chromatography with tandem mass spectrometry

Sudsakorn, Sirimas; Phatarphekar, Abhishek; O’Shea, Thomas J.; Liu, Hanlan

doi:10.1016/j.jchromb.2010.11.025

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…Protein precipitation, liquid–liquid extraction, solid‐phase extraction or combinations of these methods were commonly used for sample preparation in LC–MS/MS‐based vitamin D measurements (Contractor et al, 2017; Satoh et al, 2016; Sudsakorn, Phatarphekar, O'Shea, & Liu, 2011; Xie et al, 2011). The practical use of LC–MS/MS in clinical practice required automated sample handling and auto‐analyzers, which are widely used in clinical laboratories.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of VD2, VD3, 25(OH) D2, and 25(OH) D3 in human plasma using electrospray LC–MS/MS as well as its application to evaluate VD plasma levels in depressive, schizophrenic patients and healthy individuals

Lyu

Wang

Jin

et al. 2020

Biomedical Chromatography

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Vitamin D measurements in biological fluids by liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been widely used but remain challenging at very low concentration levels. Rapid, high recovery, sensitive and reliable measurements of vitamin D, as well as its primary metabolites using LC-MS/MS are urgently needed for a routine clinical laboratory. Herein, we reported a novel electrospray LC-MS/MS method for determining vitamin D and its primary metabolites using the supported liquid extraction method to achieve higher recoveries, with optimized pH values to achieve optimal derivatization efficiency for higher sensitivity and selected chromatographic conditions to shorten the separation time. The method has been validated with respect to selectivity, recovery, matrix effects, accuracy and precision, stabilities, carryover and dilution effects. The method has been successfully applied to quantify the VD plasma concentrations of depressive, schizophrenic patients and healthy individuals. The result showed that there were significant differences in plasma VD levels between mental disorder patients with healthy individuals, and the total VD levels in mental disorder patients were much higher than healthy individuals, which might require larger clinical samples for validation. K E Y W O R D S LC-MS/MS, PTAD derivatization optimizing, supported liquid extraction, vitamin D 1 | INTRODUCTION Vitamin D not only plays an essential role in the control of calcium homeostasis and bone mineralization in the human body, but is also related to the occurrence of benign and malignant tumors, infectious diseases, autoimmune diseases, cardiovascular diseases and mental health (Howe & Dellavalle, 2007). The typical forms of vitamin D in the human body are vitamin D2 (VD2, ergocalciferol) and vitamin D3 (VD3, cholecalciferol), which derive from plants and animals (Zmijewski, 2019). The metabolites of these two types of vitamin D are both called 25(OH) D, which is a stable storage form and serum determination marker of vitamin D in vivo. Interestingly, VD3 converts Haiyan Lyu and Suping Wang contributed equally to this work.

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Section: Resultsmentioning

confidence: 99%

Simultaneous determination of VD2, VD3, 25(OH) D2, and 25(OH) D3 in human plasma using electrospray LC–MS/MS as well as its application to evaluate VD plasma levels in depressive, schizophrenic patients and healthy individuals

Lyu

Wang

Jin

et al. 2020

Biomedical Chromatography

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“…The gold standard for the detection of small molecules is mass spectrometry (Sudsakorn et al, 2011 ). However, it requires expensive and large instrumentation as well as a high degree of training to be used correctly.…”

Section: Small Molecule Aptasensorsmentioning

confidence: 99%

Selection and Biosensor Application of Aptamers for Small Molecules

2016

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Small molecules play a major role in the human body and as drugs, toxins, and chemicals. Tools to detect and quantify them are therefore in high demand. This review will give an overview about aptamers interacting with small molecules and their selection. We discuss the current state of the field, including advantages as well as problems associated with their use and possible solutions to tackle these. We then discuss different kinds of small molecule aptamer-based sensors described in literature and their applications, ranging from detecting drinking water contaminations to RNA imaging.

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“…35 A solvent, for example, methanol, acetonitrile, ethanol ethyl acetate, or methyl tert -butyl ether, is then applied to break the interactions and to return the isolated analyte to the solution. 36–38 A sensitive equilibrium must be maintained in which the analyte is completely dissolved from the sorbent bed, but interfering substances are still retained. Positive or negative pressure is required to move solutions through the sorbent bed, whereby the application of positive pressure is also technically demanding.…”

Section: Introductionmentioning

confidence: 99%

“…44,45 In plasma and serum samples, limits of detection (LODs) achieved with a protein precipitation, an SPE, and an LC-MS/MS detection for 25(OH)D 2 and 25(OH)D 3 range from 0.2 to 3 ng/mL and limits of quantification (LOQs) range from 0.8 to 6.2 ng/mL. 33,38,46–49 Some authors reported even lower LOQ values of 0.0252 ng/mL. 38,49…”

Section: Introductionmentioning

confidence: 99%

Optimization of Automated Sample Preparation for Vitamin D Determination on a Biomek i7 Workstation

et al. 2021

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Determination of 1,25-dihydroxyvitamin D2 in rat serum using liquid chromatography with tandem mass spectrometry

Cited by 12 publications

References 19 publications

Simultaneous determination of VD2, VD3, 25(OH) D2, and 25(OH) D3 in human plasma using electrospray LC–MS/MS as well as its application to evaluate VD plasma levels in depressive, schizophrenic patients and healthy individuals

Simultaneous determination of VD2, VD3, 25(OH) D2, and 25(OH) D3 in human plasma using electrospray LC–MS/MS as well as its application to evaluate VD plasma levels in depressive, schizophrenic patients and healthy individuals

Selection and Biosensor Application of Aptamers for Small Molecules

Optimization of Automated Sample Preparation for Vitamin D Determination on a Biomek i7 Workstation

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