Determination of 1,8-dihydroxyanthranoids in senna

Electrochemical studies of danthron and the DNA–danthron interaction

“…Many analytical methods have been established for detecting varieties of anthraquinone derivatives, such as HPLC, reversed-phase HPLC (RP-HPLC), capillary chromatography, liquid chromatography-mass spectrometry (LC-MS), etc. [3][4][5]. Because of the remarkable oxidation-reduction properties of anthraquinones, electrochemical methods may be employed in the analysis for convenience.…”

Separation methods of quinonoid constituents of plants used in Oriental traditional medicines

“…Many anthraquinonoid metabolites exist along with their glycosides in roots of madder and rhubarb, and leaves and fruits of senna. Thus, samples were first extracted with aqueous alcohol, followed by hydrolysis to separate the aglycon part [21][22][23]; the two steps could be performed simultaneously also [24]. Although quinonoids have been extracted by using various solvents, to our experience [25], chloroform or dichloromethane would be the solvent of choice for getting the maximum yield of the lipophilic constituents [26]; this has been established elegantly in the work of Liu et al [24].…”

“…Simultaneous determination of 17 anthraquinones and bianthryls (sennidin and sennosides) present in senna samples was standardized by Metzger and Reif [23]. HPLC was done on Nucleosil 5 RP-8 column, using a gradient elution with solvents (A) 0.02 M KHPO 4 in water adjusted to pH 2, and (B) acetonitrile.…”

Separation procedures for the pharmacologically active components of rhubarb

“…Metzger and Reif [29] described the determination of 17 different 1,8-dihydroxyanthranoids (anthraquinones and their bianthrys) in fruits and leaves of Psenna angustifolia and Senna acutifolia. The anthranoids are extracted using a mixture of acetonitrile and a solution of sodium hydrogencarbonate.…”