Determination of 1-nitropyrene with enzyme-linked immunosorbent assay versus high-performance column switching technique

Zühlke, J.; Knopp, Dietmar

doi:10.1016/s0021-9673(98)00081-8

Cited by 10 publications

(2 citation statements)

References 25 publications

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“…Limits of detection (LOD) listed in Tables 2 and 3 correspond to an amount of 6-100 pg of the investigated nitro-PAH in the analyzed sample aliquot. They are similar to the LOD obtained in earlier HPLC-reduction-fluorescence studies using the same [25,26] or different catalyst materials like Pt/Rh [21,22] or Zn [46]. In those studies, however, only 1-9 compounds were separated and detected.…”

Section: Resultssupporting

confidence: 87%

“…Application of a catalytic reduction column before the detector allows online reduction and detection, and with the catalysts Pt/Rh or Pt on γ-alumina at high temperature (60-80°C) and a methanol/water mobile phase, high reduction rates can be achieved and maintained over months of operation [21,24,25]. So far, Pt on γ-alumina has been used for the detection of 1-nitropyrene in air particulate matter [26,27] and for 2-nitronaphthalene, 9-nitroanthracene, 3-nitrofluoranthene, 1-nitropyrene, and 6-nitrochrysene in diesel soot [25].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies

Schauer

Pöschl

2004

Analytical and Bioanalytical Chemistry

100

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Polycyclic aromatic hydrocarbons (PAH) and their nitrated derivatives (nitro-PAH) are environmental pollutants which pose a threat to human health even at low concentration levels. In this study, efficient analytical methods for the analysis of nitro-PAH and PAH (extraction, clean-up, chromatographic separation, and spectrometric detection) have been developed, characterized, and applied to aerosol samples. The separation and quantification of 12 nitro-PAH was carried out by reversed-phase high performance liquid chromatography (HPLC), on-line reduction, and fluorescence detection. The detection limits were in the range of 0.03-0.5 microg L(-1) (6-100 pg in the investigated sample aliquots), and the recovery rates from soot samples were 70-90%. Nitro-PAH and PAH concentrations have been determined for different types of soot and for urban, rural, and alpine fine air particulate matter (PM2.5). For the first time, trace amounts of nitro-PAH have been detected in a high-alpine clean air environment. The on-line reduction and fluorescence technique has been complemented by atmospheric pressure chemical ionization time-of-flight mass spectrometry (APCI-TOF-MS). The MS detection allowed the analysis of partially nitrated and oxygenated PAH in laboratory studies of the heterogeneous reaction of PAH on soot and glass fiber substrates with gaseous nitrogen oxides and ozone. It led to the tentative identification of a previously unknown nitrated derivative of the particularly toxic PAH benzo[ a]pyrene (BaP-nitroquinone), and provides the first experimental evidence that PAH-nitroquinones can be formed by reaction of PAH with atmospheric photooxidants.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Analysis of nitrated polycyclic aromatic hydrocarbons by liquid chromatography with fluorescence and mass spectrometry detection: air particulate matter, soot, and reaction product studies

Schauer

Pöschl

2004

Analytical and Bioanalytical Chemistry

100

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Polynuclear Aromatic Hydrocarbons Analysis in Environmental Samples

Fisher¹,

Schechter²

2000

Encyclopedia of Analytical Chemistry

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This chapter reviews conventional and novel methods of analysis of polynuclear aromatic hydrocarbons in environmental samples. Sampling and pretreatment techniques of air, soil and water are discussed, as well as various clean‐up and extraction methods. Most of the relevant polycyclic aromatic hydrocarbons (PAHs) separation techniques are reviewed. The principles and performance of the common PAH detection systems are presented. An introduction to novel analytical techniques, which will probably be introduced in the near future, is also provided.

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