Determination of 17β-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Sun, Mei‐Mei; Du, Lin; Gao, Shaopeng; Yuhong, Bao; Wang, Shuhao

doi:10.1016/j.steroids.2010.02.002

Cited by 40 publications

(16 citation statements)

References 20 publications

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“…Specific binding occurred in the central stem loop region with the minimum energy. (2) E2 bound to the loop formed by thymines (T-loop). A similar T-loop is presented in the circle where the E2 molecule forms a complex with the aptamer.…”

Section: Aptasensor For E2 Detectionmentioning

confidence: 99%

“…(1) E2 is one of the endocrine-disrupting steroid compound (EDC) hormones with a harmful effect on the endocrine function of the human body and aquatic organisms, and one of the most encountered endocrine-disrupting molecules in the environment. (2,3) This natural steroid hormone, which plays an important role in reproduction and sexual function, has been found in the environment (in water) and human body. (4) It is an important diagnostic marker of various chemical conditions associated with sex hormone imbalance, such as puberty, fertility, ovarian tumor, prostate cancer, and breast cancer.…”

Section: Introductionmentioning

confidence: 99%

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Functionalization of a Diamond Surface through N2 and H2 Irradiation for Estrogen (17��-estradiol) Aptamer Sensing

Suaebah¹,

Hasegawa

Buendia³

et al. 2019

Sensors and Materials

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A label-free fluorescence aptasensor for the determination of 17β-estradiol (E2) was developed. The aptasensor was prepared by the functionalization of a diamond surface using a nitrogen radical beam (NRB) system to produce amine terminals on the diamond itself. The fabrication of the sensor was performed by photolithography to produce a dot pattern, which was used as a sensing area for activated biomolecules. The supporting DNA strands were immobilized and an aptamer was hybridized to prepare a detection pair to bind with any E2 molecule, as the aptamer captures the E2 molecule naturally. The fluorescence microscopy signal was used for the detection of E2. The biomolecule activities were determined through special treatment, with three different stages evident in the fluorescence result, namely, hybridization, detection, and denaturation. When E2 was detected by the aptamer, the intensity of the fluorescence signal decreased because of the aptamer binding with the E2 molecule. E2 was detected by determining the fluorescence signal intensity of the dot pattern. When the aptamer was released from the supporting DNA and formed a complex with the E2 molecule, the intensity of the dot pattern decreased rapidly. The difference between the two signals indicated the number of E2 molecules bound to the aptamer. The proposed method is simple and powerful for the detection of small molecules with high sensitivity by utilizing the basic reaction of aptamers.

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Section: Aptasensor For E2 Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functionalization of a Diamond Surface through N2 and H2 Irradiation for Estrogen (17��-estradiol) Aptamer Sensing

Suaebah¹,

Hasegawa

Buendia³

et al. 2019

Sensors and Materials

View full text Add to dashboard Cite

show abstract

“…an urgent need to establish efficient methods for 17β-estradiol determination. Hence, various methods such as traditional chromatography [4,5], electrochemical methods [2,6], immunoassay [7,8], have been developed for the analysis of 17β-estradiol. Nevertheless, high requirement on sample pretreatment, instruments, operation, or enzymes stability greatly restricted their wide application.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric determination of 17β-estradiol based on the specific recognition of aptamer and the salt-induced aggregation of gold nanoparticles

Liu

Jia

et al. 2015

Materials Letters

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“…This estrogenic hormone is used also in human health and animal farming practices . For the determination of E2, many methods such as enzyme‐linked immunosorbent assay (ELISA) , chromatographic techniques , radioimmunoassay (RIA), microplate chemiluminescence immunoassay , electrochemical sensor , and measuring the change in fluorescence emission intensity have been reported. The increasing use of pharmaceuticals, chemicals, accidental spills, and releases of some estrogenic compounds causes the load of estrogenic substances in the aquatic environment .…”

Section: Introductionmentioning

confidence: 99%

A new molecular imprinting‐based mass‐sensitive sensor for real‐time detection of 17β‐estradiol from aqueous solution

Özgür

Yılmaz

Şener

et al. 2012

Env Prog and Sustain Energy

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The 17β‐estradiol (E2), natural steroid hormone, is one of the most potent endocrine disrupting compounds even at ng L−1 levels. Its rapid, selective and sensitive detection is intensively required. In this study, quartz crystal microbalance (QCM) sensor was prepared for real‐time monitoring of E2 in water samples, through the attachment of E2 imprinted nanoparticles, synthesized by mini‐emulsion polymerization, on the gold surface of QCM sensor. QCM sensor surface was characterized by atomic force microscopy (AFM), ellipsometer, and contact angle measurements. The specificity of the QCM nanosensor was shown by competitive adsorption of E2, stigmasterol and cholesterol. The results showed that QCM nanosensor has high selectivity and sensitivity for E2 even in a wide range of 3.67 nM–3.67 pM. The detection and quantification limits were calculated as 613 fM and 2.04 pM, respectively. According to the results, the proposed molecular imprinted QCM nanosensor is promising cost‐friendly alternative for quantification of E2 from ground water. © 2012 American Institute of Chemical Engineers Environ Prog, 32: 1164–1169, 2013

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Determination of 17β-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label

Cited by 40 publications

References 20 publications

Functionalization of a Diamond Surface through N2 and H2 Irradiation for Estrogen (17��-estradiol) Aptamer Sensing

Functionalization of a Diamond Surface through N2 and H2 Irradiation for Estrogen (17��-estradiol) Aptamer Sensing

Colorimetric determination of 17β-estradiol based on the specific recognition of aptamer and the salt-induced aggregation of gold nanoparticles

A new molecular imprinting‐based mass‐sensitive sensor for real‐time detection of 17β‐estradiol from aqueous solution

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