Determination of 6-mercaptopurine in plasma by mass spectrometry

Abstract

A sensitive and specific method for the quantitative determination of 6-mercaptopurine In plasma Is described. A variety of techniques was used to eliminate endogenous material which may Interfere with the assay. The limit of detection of the method Is 20 ng/mL.

Assay of azathioprine, 6‐mercaptopurine and a novel thiopurine metabolite in human plasma.

“…Detailed studies of azathioprine pharmacokinetics have now been conducted on renal transplant patients and these results will be reported elsewhere. Recently, Rosenfeld, Taguchi, Hillcoat & Kawal (1977) have developed a gas liquid chromatographymass spectrometric assay for 6-MP which seems to be specific although less sensitive than the present assay. The cost of instrumentation and personel however will limit its availability in clinical practice.…”

Gold Nanoclusters@Ru(bpy)32+-Layered Double Hydroxide Ultrathin Film as a Cathodic Electrochemiluminescence Resonance Energy Transfer Probe

“…6-Mercaptopurine (6-MP) is an antineoplastic chemotherapy drug with a thiol group . Various methods have contributed much to the determination of 6-MP. − Recently, it is found that the fluorescence of BSA–AuNCs can be selectively quenched by 6-MP over other thiol-containing compounds, such as glutathione and cysteine . Here, in order to evaluate the applicability and reliability of the proposed ERET sensor, it was applied for detecting 6-MP in human serum and urine samples.…”

Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

“…6-Mercaptopurine (6-MP), a sulfur analogue of adenine, is one of the main ingredients of anti-neoplastic agents, and uses its toxic properties to suppress cancers such as acute lymphoblastic leukemia and inammatory bowel diseases. 1,2 Different analytical methods have been proposed for the analysis of 6-MP, such as chemiluminescence, 3,4 electrochemical methods, [5][6][7] high performance liquid chromatography (HPLC), [8][9][10][11] mass spectrometry, 12 capillary electrophoresis (CE) 13 Raman assays, 14 and electrochemical assays. 5,15 The electrochemical determination of 6-MP has limited application in real samples due to the poor repeatability and complex electrode modication process.…”