Determination of 8-hydroxy-2′-deoxyguanosine oxidative stress biomarker using dysprosium oxide nanoparticles@reduced graphene oxide

Manavalan, Shaktivel; Rajaji, Umamaheswari; Chen, Shen-Ming; Selvin, S. Steplin Paul; Govindasamy, Mani; Chen, Shen‐Ming; Ali, M. Ajmal; Al-Hemaid, Fahad M.A.; Elshikh, Mohamed S.

doi:10.1039/c8qi00727f

Cited by 47 publications

(16 citation statements)

References 49 publications

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“…Various methods, including high-performance liquid chromatography (HPLC), with and without extraction, and enzyme-linked immunosorbent assays (ELISAs), are proposed to determine 8-OHdG in tissues and urine (Wu et al, 2004; Kawai et al, 2018). Recently, the nanotechnology-based method has been used for determination of 8-OHdG as a biomarker of oxidative stress (Manavalan et al, 2018). Contrastingly, exposure to carcinogens correlates with DNA oxidative damage and most associations of exposures are with urinary 8-OHdG (Franken et al, 2017).…”

Section: Types Of Biomarkersmentioning

confidence: 99%

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Dhama

Latheef

Dadar

et al. 2019

Front. Mol. Biosci.

220

172

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Various internal and external factors negatively affect the homeostatic equilibrium of organisms at the molecular to the whole-body level, inducing the so-called state of stress. Stress affects an organism's welfare status and induces energy-consuming mechanisms to combat the subsequent ill effects; thus, the individual may be immunocompromised, making them vulnerable to pathogens. The information presented here has been extensively reviewed, compiled, and analyzed from authenticated published resources available on Medline, PubMed, PubMed Central, Science Direct, and other scientific databases. Stress levels can be monitored by the quantitative and qualitative measurement of biomarkers. Potential markers of stress include thermal stress markers, such as heat shock proteins (HSPs), innate immune markers, such as Acute Phase Proteins (APPs), oxidative stress markers, and chemical secretions in the saliva and urine. In addition, stress biomarkers also play critical roles in the prognosis of stress-related diseases and disorders, and therapy guidance. Moreover, different components have been identified as potent mediators of cardiovascular, central nervous system, hepatic, and nephrological disorders, which can also be employed to evaluate these conditions precisely, but with stringent validation and specificity. Considerable scientific advances have been made in the detection, quantitation, and application of these biomarkers. The present review describes the current progress of identifying biomarkers, their prognostic, and therapeutic values.

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Section: Types Of Biomarkersmentioning

confidence: 99%

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Dhama

Latheef

Dadar

et al. 2019

Front. Mol. Biosci.

220

172

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“…Hence, we tested the electrochemical performance of a screen-printed electrode modified with graphene towards 8-OHdG detection, both in standard laboratory solutions (pH6 PBS) and in human saliva, and compared the results with those of unmodified SPE. To the best of our knowledge, there are only a few studies [30,31] that show the performance of SPE and SPE-Gr electrodes towards the detection of 8-OHdG; therefore, our experimental results are valuable.…”

Section: Introductionmentioning

confidence: 99%

Detection of 8-Hydroxy-2′-Deoxyguanosine Biomarker with a Screen-Printed Electrode Modified with Graphene

Varodi

Pogăcean

Coroș

et al. 2019

Sensors

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In this work we present the preparation of graphene material by exfoliation of graphite rods via pulses of current in electrolyte, containing a mixture of boric acid (0.05 M) and sodium chloride (0.05 M). The material was morphologically and structurally characterized by SEM/TEM/HR-TEM, XRD and FTIR techniques. TEM investigation of graphene flakes deposited onto carbon-coated grids allowed the visualization of thin and transparent regions, attributed to few-layer graphene (FLG), as well as thick and dark regions attributed to multi-layer graphene (MLG). The mixed composition of the material was additionally confirmed by XRD, which further indicated that the amount of FLG within the sample was around 83%, while MLG was around 17%. The performance of a screen-printed electrode (SPE) modified with graphene (SPE-Gr) was tested for 8-hydroxy-2′-deoxyguanosine detection. The graphene-modified electrode had a higher sensitivity in comparison with that of SPE, both in standard laboratory solutions (phosphate buffered saline—PBS) and in human saliva.

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“…rGO has also been combined with another metal oxide, dysprosium oxide (Dy 2 O 3 ) nanoparticles, for the determination of o 8 dGuo in human blood and urine samples by Manavalan et al [ 27 ]. Dy 2 O 3 @rGO was synthesized by a microwave-assisted synthetic route to improve the moderate electrical conductivity; furthermore, the electrocatalytic ability was synergically improved.…”

Section: Analytical Detection and Quantitation Methodsmentioning

confidence: 99%

“…UA and dopamine (DA) commonly coexist with o 8 dGuo in human metabolism and their electrochemical oxidation potentials are close, but with this method, their mixture gave three clear and well-separated oxidation peaks at 0.43 V (o 8 dGuo), 0.36 V (UA) and 0.22 V (DA), respectively. rGO has also been combined with another metal oxide, dysprosium oxide (Dy 2 O 3 ) nanoparticles, for the determination of o 8 dGuo in human blood and urine samples by Manavalan et al [27]. Dy 2 O 3 @rGO was synthesized by a microwave-assisted synthetic route to improve the moderate electrical conductivity; furthermore, the electrocatalytic ability was synergically improved.…”

Section: Gce Coated With Multi-walled Carbon Nanotubes (Mwcnts)mentioning

confidence: 99%

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

Ferenc

Váradi

Kupihár

et al. 2020

IJMS

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DNA damage plays a decisive role in epigenetic effects. The detection and analysis of DNA damages, like the most common change of guanine (G) to 8-oxo-7,8-dihydroguanine (OG), is a key factor in cancer research. It is especially true for G quadruplex structure (GQ), which is one of the best-known examples of a non-canonical DNA arrangement. In the present work, we provided an overview on analytical methods in connection with the detection of OG in oligonucleotides with GQ-forming capacity. Focusing on the last five years, novel electrochemical tools, like dedicated electrodes, were overviewed, as well as different optical methods (fluorometric assays, resonance light scattering or UV radiation) along with hyphenated detection and structural analysis methods (CD, NMR, melting temperature analysis and nanopore detection) were also applied for OG detection. Additionally, GQ-related computational simulations were also summarized. All these results emphasize that OG detection and the analysis of the effect of its presence in higher ordered structures like GQ is still a state-of-the-art research line with continuously increasing interest.

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Determination of 8-hydroxy-2′-deoxyguanosine oxidative stress biomarker using dysprosium oxide nanoparticles@reduced graphene oxide

Abstract: Electrochemical detection of 8-OHdG biomarker using Dy2O3@RGO/SPCE.

Cited by 47 publications

References 49 publications

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values

Detection of 8-Hydroxy-2′-Deoxyguanosine Biomarker with a Screen-Printed Electrode Modified with Graphene

Analytical and Structural Studies for the Investigation of Oxidative Stress in Guanine Oligonucleotides

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