Determination of a series of quinolones in pig plasma using solid-phase extraction and liquid chromatography coupled with mass spectrometric detection

Garces, Alejandra; Zerzaňová, A.; Kučera, Radim; Barrón, D.; Barbosa, J.

doi:10.1016/j.chroma.2006.09.082

Cited by 42 publications

(18 citation statements)

References 32 publications

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“…A big variation interval (Iv = 2-7 mm) in the inhibition zone width (Table 1) was a consequence of great variations in quantity of residues in some chickens. Anadon et al (1995) and Garces et al (2006) have also found big individual differences in tissue concentrations after treatment of chicken with fluoroquinolones via food or water (even 400 ng/g). Differences in measured concentrations can be caused by many factors, such as different metabolic speed, different weight of animals and, consequently, different distribution volume, etc.…”

Section: Discussionmentioning

confidence: 91%

Determination of flumequine residues in broiler chickens with HPLC and screening method

Petrović¹,

Baltić²,

Stefanović³

et al. 2009

Acta vet. (Beogr.)

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Chickens were used to examine tissue depletion of flumequine after multiple oral doses (12 mg/kg by every 24 h for 5 days). The presence of residues was detected using the microbiological screening method - plate pH 8.0 with E. coli NCIMB 11595 as a test microorganism. The tissue (muscle and liver) concentrations of flumequine were determined using HPLC/Fl method. During the 5 days dosing period, flumequine concentrations in breast muscle and liver exceeded the European Union MRLs (maximal residue limits). After the end of oral administration, hepatic concentrations of flumequine (1760- 90 ng/g) persisted for 3 days; at that time, flumequine residues were also detected in muscle tissue (980-40 ng/g). Flumequine concentrations in breast muscle and liver exceeded the MRL values only on the first day of the withdrawal period. Microbiological method - plate pH 8.0 with E. coli NCIMB 11595 revealed positive results in all samples with residue concentrations above MRL values. Two days of withdrawal period allowed time for the drug concentrations in meat and liver to decrease to an acceptable level prior to slaughter (below MRL)

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Section: Discussionmentioning

confidence: 91%

Determination of flumequine residues in broiler chickens with HPLC and screening method

Petrović¹,

Baltić²,

Stefanović³

et al. 2009

Acta vet. (Beogr.)

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“…Most of these methods use high-performance liquid chromatography (HPLC) with a variety of detectors (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16). Other methods have also been developed for the determination of ENRO, including: solid-phase spectrofluorimetry (SPF) (17); fluorescence (FL) (4,(18)(19)(20); solidphase extraction and UV-diode array detection (SPE-UV-DAD) (21); high-performance thin layer chromatography (HPTLC) (22); corona discharge ion mobility spectrometry (CD-IMS) (23); atomic absorption spectroscopy (AAS) (24); capillary electrophoresis (CE) (25,26); voltametry (27,28); and spectrophotometry (29,30).…”

Section: Introductionmentioning

confidence: 99%

Flow‐injection chemiluminescence determination of enrofloxacin using the Ru(phen)₃²⁺–Ce(IV) system and central composite design for the optimization of chemical variables

Rezaei

Mokhtari

2008

Luminescence

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The main purpose of this study was to develop an inexpensive, simple, rapid and sensitive chemiluminescence (CL) method for the determination of enrofloxacin (ENRO) using a flow-injection system. This method is based on rapid reduction of Ru(phen)(3)(3+), which is produced in the reaction between Ru(phen)(3)(2+) and acidic Ce(IV) by ENRO, producing strong CL. A central composite design (CCD) was used for optimization of the chemical variables. Regression analysis of the data from the CCD demonstrated that a second-order polynomial model is an adequate description of the surface over the factor limits studied. Optimization using CCD gave approximately four-fold better results than the single-factor-at-a-time method. Under optimal experimental conditions, the CL response was proportional to the concentration of ENRO over a wide range (0.008-3.6 microg/mL) with a correlation coefficient of 0.9986 and a detection limit of 0.003 microg/mL (3sigma). The relative standard deviation for 11 repeated determinations of 0.14 microg/mL ENRO was 4.2%. This method was successfully applied to the analysis of commercial formulations, spiked plasma and spiked poultry tissue. Sample analyses showed good recovery percentages for drugs and spiked plasma (95.1-103.9%). Recovery percentages for spiked poultry tissue were in the range 77.6-87.3%. The minimum sampling rate was 100 samples/h.

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“…CE separation of QNs has been theoretically studied [8,9] and its application to assaying QN antibiotics and their residues in various matrices, including rat liver perfusate [10], pig plasma and kidney [11 -14], chicken tissues [15 -17], milk [18,19], wastewater [20], and human urine [21], has been actively investigated. Various detection techniques, such as UV [22,23], amperometry [24], laser induced fluorescence [16], chemiluminescence [25], electrochemiluminescence [21], and mass spectrometry [13,18,26] have been hyphenated to CE for QN detection. Recently, our group [19] reported CE -potential gradient detection (PGD) determination of QNs in fortified milk samples.…”

Section: Introductionmentioning

confidence: 99%

CE determination of quinolones in the presence of bovine serum albumin

Qin

Liu

Fan

2008

J of Separation Science

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This article describes the influence of bovine serum albumin (BSA) as an additive on the capillary electrophoresis-potential gradient determination of five quinolones, enoxacin, norfloxacin, ofloxacin, fleroxacin, and pazufloxacin. With 10 mg/L of BSA present in the buffer of 30 mM Tris and 3 mM phosphoric acid at pH 9, the detection limits of the five quinolones were in the range of 0.24-0.68 mg/L, i. e. 5.8-16.5-fold lower than those obtained with the buffer devoid of BSA, and the analysis time was shortened. We suggest that the inner wall-adsorbed BSA suppresses the adsorption of quinolones and simultaneously enhances the electroosmotic flow rate. Our experiments indicated that adopting the potential gradient detection technique could eliminate the interference of the UV-active proteins on the detection of quinolones that would occur with conventional optical detection, and therefore offer high detection sensitivity. As a demonstration, the method was applied to the determination of QNs in fortified chicken muscle sample with satisfactory results.

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Determination of a series of quinolones in pig plasma using solid-phase extraction and liquid chromatography coupled with mass spectrometric detection

Cited by 42 publications

References 32 publications

Determination of flumequine residues in broiler chickens with HPLC and screening method

Determination of flumequine residues in broiler chickens with HPLC and screening method

Flow‐injection chemiluminescence determination of enrofloxacin using the Ru(phen)₃²⁺–Ce(IV) system and central composite design for the optimization of chemical variables

CE determination of quinolones in the presence of bovine serum albumin

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Determination of a series of quinolones in pig plasma using solid-phase extraction and liquid chromatography coupled with mass spectrometric detection

Cited by 42 publications

References 32 publications

Determination of flumequine residues in broiler chickens with HPLC and screening method

Determination of flumequine residues in broiler chickens with HPLC and screening method

Flow‐injection chemiluminescence determination of enrofloxacin using the Ru(phen)32+–Ce(IV) system and central composite design for the optimization of chemical variables

CE determination of quinolones in the presence of bovine serum albumin

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Flow‐injection chemiluminescence determination of enrofloxacin using the Ru(phen)₃²⁺–Ce(IV) system and central composite design for the optimization of chemical variables