“…The dissociation constant can also be used to theoretically predict the behavior of solutes in HPLC [6,7] and electromigration methods [8,9]. Capillary electrophoresis (CE) [10][11][12][13][14][15][16] has been introduced as a new micro-analytical technique for convenient and precise aqueous dissociation constant determination because of its advantages over other commonly used methods such as potentiometry [17][18][19], liquid chromatography (LC) [20][21][22], spectrophotometry [23][24][25][26], and nuclear magnetic resonance spectroscopy (NMR) [27,28]. CE relies on the principle that the solute exhibits an electrophoretic mobility continuum versus pH, which allows calculations independent of solute purity, since impurities can be effectively separated from the solutes of interest.…”