Determination of aminoglycoside antibiotics in pharmaceuticals by capillary zone electrophoresis with indirect UV detection coupled with micellar electrokinetic capillary chromatography

Ackermans, MT Mariëtte; Everaerts, FM Frans; Beckers, JL Jozef

doi:10.1016/0021-9673(92)87029-8

Cited by 83 publications

(30 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TLC and HPLC separation methods were also developed for quantitative determination of kanamycin in serum and urine [73] but the sensitivity, resolution, and accuracy of the TLC method was not sufficient for the analysis of kanamycin in biological samples. Reports on the analysis of kanamycin by CE have also been published [74][75][76]. Since kanamycin has no UV or fluorescence emission, complex formation [74], indirect UV methods [75], and precapillary derivatization [76] were employed for detection.…”

Section: Kanamycinmentioning

confidence: 99%

“…Reports on the analysis of kanamycin by CE have also been published [74][75][76]. Since kanamycin has no UV or fluorescence emission, complex formation [74], indirect UV methods [75], and precapillary derivatization [76] were employed for detection. The sensitivity of these CE meth-ods was sufficient for the analysis of bulk pharmaceuticals and their formulations where the concentration of kanamycin ranges from 1 to 250 mg/mL.…”

Section: Kanamycinmentioning

confidence: 99%

See 1 more Smart Citation

Recent progress in pharmacokinetic applications of capillary electrophoresis

Lin

Chen

2003

Electrophoresis

View full text Add to dashboard Cite

This review is a continuation of the previous reviews (Electrophoresis 1999, 20, 3259-3268; Electrophoresis 2001, 22, 4244-4248) to update the recent publications from 2001 to 2003 on pharmacokinetic studies using capillary electrophoresis (CE). During this period of time, CE remains as a unique analytical method for some studies, which would otherwise be limited by many factors, such as the sample volume, detection sensitivity, or separation power. It is particularly noticeable that the separation of chiral drugs in biological sample and the use of solid-phase extraction (SPE) as a simple and convenient means of sample preparation appear to become popular for CE-based assays. The use of CE for assessing complete pharmacokinetic information, however, did not show a significant growth during this period of time. In order to provide a broad range of view on how biological samples are analyzed by CE, this review will cover publications during the past two years on the use of CE for the analysis of drugs in biological fluids for general pharmacokinetic applications including drug monitoring and bioavailability studies.

show abstract

Section: Kanamycinmentioning

confidence: 99%

Section: Kanamycinmentioning

confidence: 99%

Recent progress in pharmacokinetic applications of capillary electrophoresis

Lin

Chen

2003

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…The pK* values of many weak acids such as acetic acid and benzoic acid (see Table 4) are strongly shifted and their values are higher than those of the often applied bases, such as imidazole. Imidazole is frequently used as co-ion for the determination of cations in the indirect UV mode in aqueous solutions [17,18]. Altria et al [19] performed separations of cations preparing a BGE by adding 1 mL pure acetic acid to 100 mL methanol containing 5 mM imidazole.…”

Section: Determination Of Pk M * Values In Water-methanol Mixturesmentioning

confidence: 99%

Capillary zone electrophoresis in methanol: Migration behavior and background electrolytes

2003

View full text Add to dashboard Cite

Nonaqueous (NA) solutions are often used as background electrolytes (BGEs) and NA solvents are added to aqueous BGEs as organic modifiers in capillary zone electrophoresis (CZE), in order to optimize the separations. This can be tricky, however, because the pH* and pK* concepts may be totally different in NA solvents, whereas often less knowledge is available concerning phenomena, such as system zones, applying NA solvents. In this paper, the concepts of pH* and pK* are considered for methanol as a solvent and pK* values are determined for several components in mixtures of water and methanol. With a mathematical model, adapted for calculations in methanol, parameters are calculated describing the fronting or tailing character of peaks and the question of peaks or dips, and the existence of system zones is discussed for pure methanol as a solvent. These aspects are experimentally verified, applying BGEs useful for the separation of cationic species in the indirect UV mode. It can be concluded that the mathematical model developed for aqueous BGEs is applicable to BGEs in methanol, too, and that the behavior of BGEs in methanol is comparable with that in water concerning the fronting or tailing character of peaks and the question of peaks and dips, although the mobilities and pK values can change significantly.

show abstract

“…Determinations of aminoglycosides by CZE using indirect detection [17] and borate complexation for direct detection [18] were reported. However, the high detection limit of 50 mg/L in these two methods did not allow them to be applied for therapeutic gentamicin monitoring.…”

Section: Introductionmentioning

confidence: 99%

Gentamicin assay in human serum by solid-phase extraction and capillary electrophoresis

Kaale¹,

Long

Fonge

et al. 2005

Electrophoresis

View full text Add to dashboard Cite

We describe the development of a capillary electrophoresis method for the determination of gentamicin C1, C1a, C2a, and C2 components in human serum. Using a weak cation-exchanger with 20 mM phosphate buffer, pH 7.4, 200 mM borate buffer, pH 9.0, and ammonia/methanol, solid-phase extraction (SPE) of gentamicin components from the human sera was performed. The extract was derivatized with 1,2-phthalic dicarboxaldehyde/mercaptoacetic acid reagent. The derivatives were separated with a background electrolyte comprising 60 mM 2-(N-cyclohexylamino)ethanesulfonic acid (CHES) buffer at pH 9.5 containing 31.6% m/v methanol, and quantified with UV-light absorption detection at 230 nm. The identity of the gentamicin components was confirmed by mass spectrometry. The SPE recovery of the gentamicin ranged from 78% to 93%. The calibration curves were linear from the concentration limit of quantitation (LOQ) to 30 mg/L for the gentamicin mixture. The LOQ for gentamicin C1 was 0.33 mg/L, for C2a 0.23 mg/L, C2 0.25 mg/L, C1a 0.27 mg/L and the concentration limit of detection (LOD) for C1 was 0.15 mg/L, C2a 0.11 mg/L, C2 0.12 mg/L, C1a 0.13 mg/L. Intra-assay relative standard deviation (RSD) values were for C1 (5%), C1a (7%), C2 (6.5%) and C2a (9%); inter-assay RSD values were for C1 (11%), C1a (13.3%), C2 (15%) and C2a (14%). The Pearson's correlation between capillary electrophoresis and immunoassay revealed a linear relationship between these two techniques with r = 0.9. This method for determination of gentamicin C1, C1a, C2a, and C2 in human serum can thus be used in the entire therapeutic concentrations range of gentamicin.

show abstract

Determination of aminoglycoside antibiotics in pharmaceuticals by capillary zone electrophoresis with indirect UV detection coupled with micellar electrokinetic capillary chromatography

Cited by 83 publications

References 17 publications

Recent progress in pharmacokinetic applications of capillary electrophoresis

Recent progress in pharmacokinetic applications of capillary electrophoresis

Capillary zone electrophoresis in methanol: Migration behavior and background electrolytes

Gentamicin assay in human serum by solid-phase extraction and capillary electrophoresis

Contact Info

Product

Resources

About