Determination of Acetazolamide in Biological Fluids by Reverse-Phase High-Performance Liquid Chromatography

Determination of acetazolamide by graphene paste electrode

“…About 80% of the drug is excreted by tubular secretion of the anionic species, and 70-90% of the administered dose is recovered unchanged within 24 h [28]. The determination of ACZ in biological fluids is usually carried out by sol-gel optical biosensor [29], polarography [30], enzymatic assay [31], electron-capture GLC [32], molecular imprinted polymer (MIP) [33], carbon paste electrode modified with gold nanoparticle [34] and high-performance liquid chromatography [35][36][37][38][39]. However, most of the above methods require several time consuming manipulation steps, sophisticated instruments and special training.…”

Determination of acetazolamide in dosage forms by high performance liquid chromatography

“…Preliminary runs based upon literature review (Chapron and White, 1984) using the p-Bondapak C,* column and a acetonitrilc:methanoI:0.05 M sodium acetate (3:2:95) mixture as the mobile phase were unsatisfactory. Broad peaks were obtained and a need for adjustment in the mobile phase was indicated.…”

“…Several high performance liquid chromatographic (HPLC) assays have been published for the determination of acetazolamide in biological fluids (Bayne et al, 1975;Hossie et af., 1980;Chambers et al, 1981;Chapron and White, 1984), but no work seems to have been reported for its determination by HPLC in pharmaceutical dosage forms. The present work describes a modified HPLC method of analysis for acetazolamide in dosage forms.…”

Differential effects of flurbiprofen and aspirin on acetazolamide disposition in humans.

“…(Chapron & White, 1984;Cham et al, 1979;Symmons et al, 1983). Creatinine clearance was measured for each subject during the first 24 h of each study phase.…”