Determination of Acetylcysteine in Pharmaceutical Samples by Silicomolybdenum Blue Spectrophotometry

Abstract

An accurate and fast spectrophotometric method for the determination of acetylcysteine by silicomolybdenum blue has been established. The various effect factors on the determination of acetylcysteine by silicomolybdenum blue spectrophotometry are investigated in detail. The results show that under the optimum reaction conditions, SiO 3 2reacts with Mo 7 O 24 6to form silicon molybdenum heteropoly acid (H 4 Si(Mo 3 O 10 ) 4 ). Then H 4 Si(Mo 3 O 10 ) 4 is reduced by hydrosulfuryl (-SH) in acetylcysteine to form silicomolybdenum blue (H 4 Si(Mo 3 O 10 ) 2 (Mo 3 O 9 ) 2 ). The absorbance of silicomolybdenum blue is measured at the maximal absorption wavelength of 735 nm, and the content of acetylcysteine can be calculated based on this absorbance. A good linear relationship is obtained between the absorbance of silicomolybdenum blue and the concentration of acetylcysteine in the range of 5.040~25.20 mg·mL -1 . The linear regression equation is A = 0.0272 + 21.484C (mg·mL -1 ), with a linear correlation coefficient of 0.9995. This proposed method has been successfully applied to the determination of acetylcysteine in pharmaceutical samples, and the results agree well with those obtained by pharmacopoeial method.

Biomimetic Synthesis and Mechanism of Hydroxyapatite Crystals by Biotemplate of Small Intestinal Submucosa and Bacterial Cellulose

“…X‐ray photoelectron spectroscopy (XPS, ESCALAB 250 Xi, Inc. Thermo Fisher USA) and scanning electron microscope‐energy dispersive spectrometer (SEM‐EDS) was used to determine the valence states of the elements and the changes in elemental composition and content after mineralization. [ 21 ] TG curves of the lyophilized samples were recorded the pyrolysis carbonization process using thermogravimetric analysis (TGA, DTG‐60AH). Under atmosphere, samples were heated in the range of 30–1000 °C at a heating rate of 10 °C min −1 .…”