Determination of Acetylsalicylic Acid in Pharmaceutical Drugs by TLC With Densitometric Detection in Uv

A Simple and Cost-Effective TLC-Densitometric Method for the Quantitative Determination of Acetylsalicylic Acid and Ascorbic Acid in Combined Effervescent Tablets

“…What is important is that the proposed method also allows for the detection of SA in pharmaceutical preparations. Just like other papers [5,7,34], during the initial step of the conducted studies, the presence of a small amount of SA in the examined sample can be observed. Over time, the amount of SA can increase slightly at the expense of ASA.…”

“…The separations of AA, SA, and ASA were achieved by choosing the optimal conditions for the TLC analysis. To select the one that will enable the best separation of the above-mentioned substances, the following (12 mobile phases) were tested: mobile phase A: glacial acetic acid:acetone:methanol:benzene (0.5:0.5:2:7, v/v / v/v ) [32]mobile phase B: chloroform:ethanol 96%:water (3:6:1.5, v/v/v ) [24]mobile phase B1: chloroform:ethanol 96% (3:6, v/v )mobile phase B2: chloroform:ethanol (5:5, v/v )mobile phase B3: chloroform:ethanol 96%:glacial acetic acid (5:5:0.03, v/v/v )mobile phase B4: chloroform:ethanol 96%:glacial acetic acid (5:4:0.03, v/v/v )mobile phase C: methylene chloride:ethyl acetate:ethanol 96% (5:5:1, v/v/v ) [33]mobile phase D: n -hexane:diethyl ether:80% acetic acid (7:2:1, v/v/v ) [5]mobile phase E: n -hexane:acetone:glacial acetic acid (5:5:0.09, v/v/v )mobile phase F: n -hexane:acetone:glacial acetic acid (5:5:0.5, v/v/v )mobile phase G: acetone: n -hexane:glacial acetic acid (5:5:0.06, v/v/v )mobile phase G1: n -hexane-acetone-glacial acetic acid in the following volume composition: 7 mL:2 mL:1 drop [8]. …”

“…The levels of the factors investigated and the experimental design matrix (2 3 ) are shown in Table 3 and Table 4, respectively. The ways of calculation of the effects ( E ) characterizing the particular individual factors and rank probabilities [37] were presented earlier [5,8,30,35,36,38].…”

“…So far, various analytical techniques have been developed for the determination of ASA in pharmaceutical tablets such as UV-spectrophotometry [2], spectrofluorometry [3], HPLC (high-performance liquid chromatography) [4], and TLC (thin-layer chromatography) [3,5,6,7,8]. The method recommended by Polish and United States Pharmacopoeias for ASA is HPLC with UV detection [9,10].…”

“…In our papers, we have previously investigated the optimum conditions for the separation of caffeine, ethoxybenzamide, and acetylsalicylic acid from its related substance, and salicylic acid by normal phase thin layer chromatography (NP-TLC) with densitometry [5,8]. It was ascertained that the best conditions to separate acetylsalicylic acid from salicylic acid enables the mobile phase: n-hexane-diethyl ether-acetic acid (80%) in a volume ratio of 7:2:1 [5]. The second mixture of n -hexane-acetone-glacial acetic acid in a volume composition of 7 mL:2 mL:1 drop was suitable for the separation of caffeine, ethoxybenzamide, and acetylsalicylic acid from its related substance [8].…”