Determination of acute toxicity of “Bendamin” drug in laboratory animals

Varkholiak, I. S.; Gutyj, B. V.

doi:10.32718/nvlvet9243

Cited by 9 publications

(9 citation statements)

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“…За впливу етилметилгідроксипіридину сукцинату встановлено зниження в кардіоміоцитах специфічного білка, що прискорює апоптоз. Також відбувається модулювання трансмембранних іонних струмів: сповільнюється повільний струм через кальцієві канали, відвертається блокада швидких натрієвих каналів та блокада швидко активованого компонента калієвого струму затриманого випрямлення (JKR) (Varkholiak & Gutyj, 2018;Varkholiak et al, 2019;.…”

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The effect of the drug “Bendamin” on the biochemical parameters of the blood of dogs in heart failure

Varkholiak

Gutyj

2021

SMLNUVMB

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Experimental studies obtained in recent years indicate the urgency of developing drugs of complex action to prevent the development of cardiovascular disease in dogs. This requires a much deeper study of the pathogenesis of heart failure, including cardiomyopathy. The aim of the study was to investigate the effect of the drug “Bendamin” on the biochemical parameters of the blood of dogs in heart failure. Two groups of dogs were formed for research: control (healthy) and experimental (sick). The 30-day experiment included 10 dogs of the control group and 10 dogs of the experimental (sick) breed of spaniel and dog 10–12 years of age. Treatment regimen for sick dogs: furosemide was administered orally on an empty stomach at 2 mg/kg every 12 hours; enalapril was administered orally at 0.5 mg/kg every 12 hours; bendamine was administered orally on an empty stomach at 20 mg/kg every 24 hours. It is recommended to divide the dose into two doses. An increase in the activity of aminotransferases (ALT by 59.2 %, AST – by 51.9 %) was found in the serum of dogs at heart failure. An increase in alkaline phosphatase activity was also found, which in the complex is an additional confirmation of changes in the heart muscle. Lactate dehydrogenase activity increased in the blood of sick dogs by 23.5 %. Minor changes in the content of total bilirubin and urea in the serum of sick dogs indicate the initial stage of liver pathology with impaired liver function due to congestion in the small circulation. This is also indicated by a decrease in the level of total protein in the blood of sick dogs, which was respectively 57.2 ± 3.57 g/l against the control group of 63.5 ± 4.15 g/l. On the basis of cardiac dysfunction and on the background of congestion, the function of not only the liver but also the kidneys is impaired (the so-called cardiorenal syndrome in heart failure). This is indicated by an increase in urea and creatinine by 38.5 and 36.9 %, respectively, compared with animals in the control group. The use of the drug “Bendamin” alleviated the manifestations of functional failure of the heart, liver and kidneys, as indicated by biochemical studies. Studies confirm the feasibility of using the drug “Bendamin” in dogs with the development of heart failure. The use of the drug “Bendamine” in patients with heart failure in dogs had a positive effect on the restoration of hematopoiesis and the normalization of biochemical parameters of the blood.

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The effect of the drug “Bendamin” on the biochemical parameters of the blood of dogs in heart failure

Varkholiak

Gutyj

2021

SMLNUVMB

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“…Розробка, дослідження та впровадження нових кардіопрепаратів для профілактики і лікування тварин за серцево-судинних захворювань, у механізмі яких відбувається розвиток оксидативного стресу, є на часі і мають значну перспективу. У цьому плані доцільним є використання у ветеринарній медицині за серцевої недостатності у тварин інгібіторів фосфодиестерази-3 та антиоксидантів (Varkholiak & Gutyj, 2018;. Саме тому актуальним є розробка вітчизняного кардіопрепарату для собак за патологій серцево-судинної системи та дослідження його фармакотоксикологічних параметрів.…”

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The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy

et al. 2020

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Experimental studies obtained in recent years indicate the urgency of developing drugs of complex action to prevent the development of cardiovascular disease in dogs. This requires a much deeper study of the pathogenesis of heart failure, including cardiomyopathy. The aim of the study was the effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy. To reproduce heart failure in rats, an experimental model was used by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks. The study was performed on white sexually mature young male Wistar rats weighing 180–200 g, which were kept on the standard diet of the institute vivarium of the State Research Control Institute of Veterinary Drugs and Feed Additives. For the study, three groups of rats of 6 animals in each were formed: control group – intact animals; experimental group R1, in which animals were simulated doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin; experimental group R2, in which animals after injection of doxorubicin, intragastrically administered the drug “Bendamine” at a dose of 20 mg/kg. According to studies, doxorubicin intoxication in rats enhances LPO processes, as indicated by an increased content of lipid hydroperoxides by 47.4 %, diene conjugates – by 21.4 % and TBA-active products – by 24.9 % for animals of the control group. Doxorubicin cardiomyopathy in rats has a decrease in the activity of antioxidant enzymes in the blood of animals. In particular, the activity of catalase decreases by 36.9 % (P ≤ 0.001), and the activity of SOD – by 20.3 % (P ≤ 0.001). In the study of the activity of the glutathione link of the antioxidant defense system, similar changes were found as in the study of the activity of catalase and SOD. The use of the drug “Bendamine” in the rats of the experimental group helped to inhibit the excessive formation of lipid peroxidation products and increase the activity of both enzymatic and non-enzymatic parts of the antioxidant system.

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“…У попередніх наших дослідженнях встановлено, що препарат "Бендамін" після введення в шлунок білим мишам та щурам у максимально допустимій кількості не викликає клінічних ознак отруєння та відхилень у поведінці (Varkholiak & Gutyj, 2018).…”

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Determination of the chronic toxicity of preparation “Bendamin” on laboratory animals

Varcholyak¹,

Гутый²

2019

TAVM

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In the process of creating and introducing each new preparation one of the stages is its toxicological studies with the clarification of the parameters of acute and chronic toxicity in white rats in the long term of its intragastric introduction. The aim of the work was to determine the parameters of the chronic toxicity of the cardiological preparation "Bendamin" on laboratory animals in a laboratory experiment. The studies were carried out on white, sexually-mature, young male rats of the Vistar line of 2-3 months old, weighing 170-185 g, which were kept on a standard ration of the Institute Vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Additives. Four experimental groups of white rats were formed. The first experimental group served as a control. Other experimental groups were prescribed "Bendamin", respectively, in therapeutic, 5-fold and 10-fold doses. The preparation in the above doses was given at a specific time, daily, during 30 days, peroral, fasting, using a probe for laboratory animals. During the research period to determine the chronic toxicity of "Bendamin", no probable changes in the behavior of the experimental animals were observed. It should be noted the slight inhibition of the organism state of the being studied group of rats receiving a 10-fold dose of the preparation. In the investigated group of rats, which was injected the preparation in the 10-fold dose, inhibition of protein synthesis and detoxification function of the liver was established. There is also a violation of the functional state of the liver, which indicates an increase in the activity of aminotransferases in their blood. In the study of the relative weight coefficients of the mass of the internal organs, it was found that the introduction of the "Bendamin" preparation in the 10-fold dose led to a significant change in the relative weight of the liver and the spleen. The "Bendamin" preparation in the therapeutic and 5-fold doses contributed to a slight increase in the weighting coefficient. Lung weight loss occurred at introduction of the preparation in the 5-and 10-fold doses. According to State Standard 12.1.007-76, "Bendamin", for intragastric introduction, according to the degree of hazard belongs to the fourth-class toxicity-low toxicity substances (DL 50 more than 50,000 mg/kg body weight).

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Determination of acute toxicity of “Bendamin” drug in laboratory animals

Cited by 9 publications

References 4 publications

The effect of the drug “Bendamin” on the biochemical parameters of the blood of dogs in heart failure

The effect of the drug “Bendamin” on the biochemical parameters of the blood of dogs in heart failure

The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy

Determination of the chronic toxicity of preparation “Bendamin” on laboratory animals

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