In the process of creating and introducing each new preparation one of the stages is its toxicological studies with the clarification of the parameters of acute and chronic toxicity in white rats in the long term of its intragastric introduction. The aim of the work was to determine the parameters of the chronic toxicity of the cardiological preparation "Bendamin" on laboratory animals in a laboratory experiment. The studies were carried out on white, sexually-mature, young male rats of the Vistar line of 2-3 months old, weighing 170-185 g, which were kept on a standard ration of the Institute Vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Additives. Four experimental groups of white rats were formed. The first experimental group served as a control. Other experimental groups were prescribed "Bendamin", respectively, in therapeutic, 5-fold and 10-fold doses. The preparation in the above doses was given at a specific time, daily, during 30 days, peroral, fasting, using a probe for laboratory animals. During the research period to determine the chronic toxicity of "Bendamin", no probable changes in the behavior of the experimental animals were observed. It should be noted the slight inhibition of the organism state of the being studied group of rats receiving a 10-fold dose of the preparation. In the investigated group of rats, which was injected the preparation in the 10-fold dose, inhibition of protein synthesis and detoxification function of the liver was established. There is also a violation of the functional state of the liver, which indicates an increase in the activity of aminotransferases in their blood. In the study of the relative weight coefficients of the mass of the internal organs, it was found that the introduction of the "Bendamin" preparation in the 10-fold dose led to a significant change in the relative weight of the liver and the spleen. The "Bendamin" preparation in the therapeutic and 5-fold doses contributed to a slight increase in the weighting coefficient. Lung weight loss occurred at introduction of the preparation in the 5-and 10-fold doses. According to State Standard 12.1.007-76, "Bendamin", for intragastric introduction, according to the degree of hazard belongs to the fourth-class toxicity-low toxicity substances (DL 50 more than 50,000 mg/kg body weight).

The effect of ‘Vitosept’ drug, based on sodium hypochlorite solution with high purity, obtained in a specially developed membraneless flow electrolyzer, on the embryotoxic and teratogenic properties in rats was investigated. To determine the embryotoxic effect of ‘Vitosept’ on the development of white rats offspring of the 1st generation, control and three experimental groups (G1, G2, G3) were formed from pregnant females. The females of the control group with a blunt probe were injected daily for 30 days with 5 ml of isotonic sodium chloride solution, and experimental ones with 5 ml of ‘Vitosept’ drug with different concentration of high purity sodium hypochlorite: Group I (G1) — 50 mg/l; Group II (G2) — 100 mg/l; Group III (G3) — 500 mg/l. The animals were observed. During the observation the condition and behavior of the females, the dynamics of body weight change, duration of pregnancy, and the course of birth were monitored. The results of the experiment were recorded after the slaughter of pregnant females (20th day of pregnancy) and in the postnatal period of development of the offspring. Studies have shown that the use of different concentrations of the drug ‘Vitosept’ in rats for 30 days before and during pregnancy has no embryotoxic and teratogenic effects. According to the indicators of the total, pre- and postimplantation lethality of embryos, there were no reliable changes in the structure and morphometry of internal organs and tissues in 20-day-old fetuses, and their development corresponded to the terms of pregnancy. There was no significant difference between the fertility of female rats in the test and control groups. The average number of fetuses per female was within 9 animals. The rats obtained from the females of the experimental groups were viable and did not lag behind in growth and development compared with the control animals, which generally characterizes the studied drug ‘Vitosept’ as non-toxic, lacking embryotoxic and teratogenic action