Determination of adenosine, cordycepin and ergosterol contents in cultivated Antrodia camphorata by HPLC method

Chang, Ching-Jin; Lue, Ming-Yong; Pan, Tzu‐Ming

doi:10.38212/2224-6614.2569

Cited by 14 publications

(11 citation statements)

References 19 publications

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“…For EGR detection, 0.05 g of freeze-dried mycelia powder was suspended in absolute ethanol following the extraction method from Chang et al [ 34 ] to detect the ERG content using HPLC. The ERG was separated by an Inertsil ODS-3 C18 column (250 mm × 4.6 mm, 5 μm), using isocratic elution with 100% methanol as the mobile phase and a flow rate of 1.0 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Effect of Static Magnetic Field on Monascus ruber M7 Based on Transcriptome Analysis

Yang

Zhou

Dai

et al. 2021

JoF

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The effects of a static magnetic field (SMF) on Monascus ruber M7 (M. ruber M7) cultured on potato dextrose agar (PDA) plates under SMF treatment at different intensities (5, 10, and 30 mT) were investigated in this paper. The results revealed that, compared with the control (CK, no SMF treatment), the SMF at all tested intensities did not significantly influence the morphological characteristics of M. ruber M7, while the intracellular and extracellular Monascus pigments (MPs) and extracellular citrinin (CIT) of M. ruber M7 were increased at 10 and 30 mT SMF but there was no impact on the MPs and CIT at 5 mT SMF. The transcriptome data of M. ruber M7 cultured at 30 mT SMF on PDA for 3 and 7 d showed that the SMF could increase the transcriptional levels of some relative genes with the primary metabolism, including the carbohydrate metabolism, amino acid metabolism, and lipid metabolism, especially in the early growing period (3 d). SMF could also affect the transcriptional levels of the related genes to the biosynthetic pathways of MPs, CIT, and ergosterol, and improve the transcription of the relative genes in the mitogen-activated protein kinase (MAPK) signaling pathway of M. ruber M7. These findings provide insights into a comprehensive understanding of the effects of SMF on filamentous fungi.

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Section: Methodsmentioning

confidence: 99%

Effect of Static Magnetic Field on Monascus ruber M7 Based on Transcriptome Analysis

Yang

Zhou

Dai

et al. 2021

JoF

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“…The cordycepin-rich supernatant was filtered by 0.45-µm membrane, and the cordycepin and adenosine levels were measured by HPLC analysis with RP-18 column modifying the method of Chang, Lue, and Pan (2005) [23]: the mobile phase was a mixture of methanol and deionized water, the column temperature was 40 • C, the flow rate was 1.0 mL/min, and the detector was set at 260 nm.…”

Section: High Performance Liquid Chromatography (Hplc) Assay For Cordycepin Levelmentioning

confidence: 99%

Polysaccharides Obtained from Cordyceps militaris Alleviate Hyperglycemia by Regulating Gut Microbiota in Mice Fed a High-Fat/Sucrose Diet

Lee

Chen

Hsu

et al. 2021

Foods

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Polysaccharides isolated from fungus Cordyceps militaris display multi-biofunctions, such as immunostimulation, down-regulation of hyperlipidemia, and anti-cancer function. The occurrence of obesity and metabolic syndrome is related to the imbalance of gut microbiota. In this study, the effects of C. militaris and its fractions on modifying metabolic syndrome in mice were evaluated. Mice were fed a high-fat/high-sucrose diet (HFSD) for 14 weeks to induce body weight increase and hyperlipidemia symptoms in mice, and then the mice were simultaneously given a HFSD and C. militaris samples for a further 8 weeks. The results indicated that the fruit body, polysaccharides, and cordycepin obtained from C. militaris had different efficacies on regulating metabolic syndrome and gut microbiota in HFSD-treated mice. Polysaccharides derived from C. militaris decreased the levels of blood sugar and serum lipids in mice fed HFSD. In addition, C. militaris-polysaccharide treatment obviously improved intestinal dysbiosis through promoting the population of next generation probiotic Akkermansia muciniphila in the gut of mice fed HFSD. In conclusion, polysaccharides derived from C. militaris have the potential to act as dietary supplements and health food products for modifying the gut microbiota to improve the metabolic syndrome.

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“…Nutrient composition of ATC The contents of ergosterol, cordycepin, and cordycepic acid, and Cordyceps polysaccharide in ATC were determined by high-performance liquid chromatography (15). The amino acid content was determined using a HITACHI L-8800 automatic amino acid analyzer (HITACHI Co., Tokyo, Japan).…”

Section: Chemical Analysesmentioning

confidence: 99%

Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis

Jiang

et al. 2020

Food & Nutrition Research

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Background As a major disease affecting dairy cow production worldwide, bovine mastitis is caused by a variety of pathogenic microorganisms that eventually cause mammary gland inflammation. Acremonium terricola culture (ATC) is a new type of affordable feed additive produced by the solid fermentation of A. terricola isolated from Cordyceps gunnii and exerted its anti-inflammatory effect. Objectives To evaluate the protective effects of ATC on mastitis and investigate its active mechanism, a lipopolysaccharide (LPS)-induced rat mastitis model was used in two experiments. Design In Experiment 1, a total of 40 female Sprague–Dawley rats were used to determine the optimal supplementary dose of ATC via gavage trial. In Experiment 2, we examined the effects of an optimal dose of ATC on LPS-induced mastitis in rats. Results The results of Experiment 1 showed that administration of ATC improved growth performance and antioxidant functions in the serum and the liver, as well as immunoglobulin A, G, and M levels in rat serum, and it decreased the content of alanine aminotransferase, aspartate aminotransferase, triglyceride, cholesterol, low-density lipoprotein, and serum urea nitrogen in rat serum; a dosage of 250–1,250 mg/kg/day was shown to be high enough to be effective. The results of Experiment 2 indicated that ATC can relieve the inflammatory reaction of mammary glands in rats, and the LPS-induced expression of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase significantly decreased after ATC treatment. Moreover, our results demonstrated that ATC markedly enhanced the activity of antioxidase in this rat mastitis model. The results of Western blot analysis revealed that ATC could suppress the expression of toll-like receptor 4, phosphorylation of extracellular signal-regulated kinase, and activity of c-Jun N-terminal kinase in the LPS-stimulated mastitis model. Conclusion Taken together, ATC was shown to exert its anti-inflammatory effect by blocking mitogen-activated protein kinase signaling pathways. These results demonstrate that ATC exerts anti-inflammatory and antioxidant effects in mastitis prevention.

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Determination of adenosine, cordycepin and ergosterol contents in cultivated Antrodia camphorata by HPLC method

Cited by 14 publications

References 19 publications

Effect of Static Magnetic Field on Monascus ruber M7 Based on Transcriptome Analysis

Effect of Static Magnetic Field on Monascus ruber M7 Based on Transcriptome Analysis

Polysaccharides Obtained from Cordyceps militaris Alleviate Hyperglycemia by Regulating Gut Microbiota in Mice Fed a High-Fat/Sucrose Diet

Acremonium terricola culture plays anti-inflammatory and antioxidant roles by modulating MAPK signaling pathways in rats with lipopolysaccharide-induced mastitis

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