Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution

Abstract

The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds.

Determination of Aflatoxin B1 in Smokeless Tobacco Products by Use of UHPLC-MS/MS

“…Our selection of the m / z 313.32>241.22 transition for AFB 1 quantitation is consistent with its use in other LC-MS/MS methods. 16,21,22 Using an analogous approach we also identified quantitation and confirmation MS/MS transitions for AFB 2 , G 1 , G 2 , as well as internal standardization MS/MS transitions for their respective 13 C 17 -labeled analogues.…”

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS

“…A SIDA method for aflatoxins in different food products was developed by Cervino et al [31] who gained [ 2 H 2 ]-AFB 2 and [ 2 H 2-4 ]-AFG 2 by a catalytic deuteration of AFB 1 and AFG 1 . For the determination of the four major aflatoxins in animal feed by solid phase extraction, U-[ 13 C 17 ]-AFB 1 was used as the internal standard for all analytes [32]. …”

Collision‐induced dissociation of aflatoxins

“…[14][15][16][17][18][19] However, with the appearance of soft ionization mass spectrometry (MS) techniques such as atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI), which can be easily coupled with liquid chromatography (LC), the application of LC/MS for the determination of mycotoxins has been increasing. [20][21][22][23] In this article we report the detailed fragmentation study of the four most common aflatoxins: B1, B2, G1 and G2. To our knowledge no detailed reports on the fragmentation properties and fragmentation pathways of this important and intriguing class of mycotoxins have been published.…”