Determination of aflatoxins in animal feeds by HPLC with multifunctional column clean-up

Khayoon, Wejdan Shakir; Saad, Bahruddin; Yan, Chew Bee; Hashim, Nor Hasani; Ali, Abdussalam Salhin Mohamed; Salleh, Muhammad Idiris; Salleh, Baharuddin

doi:10.1016/j.foodchem.2009.05.082

Cited by 114 publications

(65 citation statements)

References 23 publications

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“…Four samples of the total malt samples were positive for AFG2 and AFG1, and traces of AFB1 and AFB2 were detected. Khayoon et al (2010) verified the occurrence of AFB1, B2, G1 and G2 in 42 animal feeds, comprising corn (16), soybean meal (8), mixed meal (13), sunflower, wheat, canola, palm kernel, copra meals (1 each). The results showed that eight samples (19%) were contaminated with aflatoxins, ranging from 6.5 to 101.9 ng g −1 .…”

Section: Factors Promoting Contamination In Aflatoxins and Occurrencementioning

confidence: 69%

Aflatoxins: Contamination, Analysis and Control

Dors¹,

Caldas²,

Feddern³

et al. 2011

Aflatoxins - Biochemistry and Molecular Biology

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Section: Factors Promoting Contamination In Aflatoxins and Occurrencementioning

confidence: 69%

Aflatoxins: Contamination, Analysis and Control

Dors¹,

Caldas²,

Feddern³

et al. 2011

Aflatoxins - Biochemistry and Molecular Biology

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“…The eluate was evaporated to dryness at 40 °C under a stream of nitrogen. The residue was derivatized with trifluoroacetic acid according to the method of Khayoon et al (2010). Then, 1 ml of acetonitrile/water (30/70, v/v) was added.…”

Section: Afb 1 Analysismentioning

confidence: 99%

Potential natural exposure of endangered red-crowned crane (Grus japonensis) to mycotoxins aflatoxin B1, deoxynivalenol, zearalenone, T-2 toxin, and ochratoxin A

Liu

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:A survey was conducted to determine whether mycotoxins were present in the foods consumed by red-crowned cranes (Grus japonensis) in the Yancheng Biosphere Reserve, China. Collected in the reserve's core, buffer, and experimental zones during overwintering periods of 2013 to 2015, a total of 113 food samples were analyzed for aflatoxin B 1 , deoxynivalenol, zearalenone, T-2 toxin, and ochratoxin A using high performance liquid chromatography (HPLC). The contamination incidences vary among different zones and the mycotoxins levels of different food samples also presented disparity. Average mycotoxin concentration from rice grain was greater than that from other food types. Among mycotoxin-positive samples, 59.3% were simultaneously contaminated with more than one toxin. This study demonstrated for the first time that red-crowned cranes were exposed to mycotoxins in the Yancheng Biosphere Reserve and suggested that artificial wetlands could not be considered good habitats for the birds in this reserve, especially rice fields.

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“…Extraction of AFs was done according to Khayoon et al (2010), where 20 g of colonized cornmeal sample was extracted with 80 mL acetonitrile: H 2 O (9:1; v/v) mixture for 30 min by shaking under high speed, and then filtering using a No. 4 Whatman filter paper.…”

Section: Inoculation Of Spores and Sample Extractionmentioning

confidence: 99%

“…The peaks were observed using an UV detector with a wavelength of 360 nm. The sample peaks were compared with the standard curve to determine the concentration of AFB 1 and AFB 2 produced (Khayoon et al, 2010).…”

Section: Moniliformin (Mon)mentioning

confidence: 99%

Mycotoxins Production by Fusarium and Aspergillus Species Isolated from Cornmeal

Zainudin¹,

Perumal²

2015

IJAB

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Corn is a vital food source for human consumption, animal feed as well as industrial processing. However, corn faces repeated spoilage and contamination by a huge range of fungi especially by Fusarium and Aspergillus species. These fungi are known of producing mycotoxins such as fumonisins (FBs), moniliformin (MON), zearalenone (ZEA), beauvericin (BEA) and aflatoxins (AFs). The main objective of this study was to quantify the mycotoxins produced by both fungi associated with cornmeal in Malaysia. The extracted mycotoxins were qualitatively analyzed using A. salina bioassay, and quantitatively using Ultra-fast Performance Liquid Chromatography (UFLC). Three hundred and fourteen isolates of microfungi were obtained, 90.5% isolates belonged to Aspergillus species, namely A. flavus (76.8%), A. niger (7.6%), A. nidulans (4.5%) and A. fumigatus (1.6%). Another 9.6% isolates were Fusarium species, identified as F. verticillioides (4.5%), F. semitectum (3.2%) and F. proliferatum (1.9%). As for the mycotoxin analysis, out of 40 isolates of Aspergillus, 29 isolates produced AFB 1 and only two isolates produced AFB 2 . Fifteen out of 16 Fusarium isolates produced MON, 12 isolates produced BEA and all isolates produced FB 1 , but none of them produced ZEA. The analysis of A. salina revealed that all the five mycotoxins extracts were toxic to the brine shrimp despite the concentration of the mycotoxins.

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Determination of aflatoxins in animal feeds by HPLC with multifunctional column clean-up

Cited by 114 publications

References 23 publications

Aflatoxins: Contamination, Analysis and Control

Aflatoxins: Contamination, Analysis and Control

Potential natural exposure of endangered red-crowned crane (Grus japonensis) to mycotoxins aflatoxin B1, deoxynivalenol, zearalenone, T-2 toxin, and ochratoxin A

Mycotoxins Production by Fusarium and Aspergillus Species Isolated from Cornmeal

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