Determination of aflatoxins in food products by the ELISA method

Leszczyńska, Joanna; Masłowska, J.; Owczarek, Aleksandra; Kucharska, Urszula

doi:10.17221/6567-cjfs

Cited by 34 publications

(19 citation statements)

References 15 publications

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“…Nduti et al [26] recently analysed aflatoxin B1 in cereals and other agricultural produce including sun-dried grains of maize and millet, maize flour and millet flour samples by PCR, a modified procedure similar to the ELISA methods reported by other researchers [50,58,59,61]. The samples were transported immediately after sampling in cool boxes to an ISO 1705 accredited by Kenya Bureau of Standards laboratory and stored at −20°C until analysis was started.…”

Section: Analysis and Identification Of Aflatoxin B1mentioning

confidence: 99%

“…After, 100 μl of the sample plus conjugate mixture was transferred to antibody-coated microwells and the mixtures incubated for 15 min. The method of Leszczynka et al [61] was modified by using a specific conjugate mixture, thus eliminating the need for wells pre-washed with phosphate buffer solution (PBS). The PBS cleans the unbound proteins but also reduces sensitivity at the enzyme reaction site [62].…”

Section: Analysis and Identification Of Aflatoxin B1mentioning

confidence: 99%

See 1 more Smart Citation

Aflatoxin B1: Chemistry, Environmental and Diet Sources and Potential Exposure in Human in Kenya

Lalah¹,

Omwoma²,

Orony³

2020

Aflatoxin B1 Occurrence, Detection and Toxicological Effects

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Cancer incidences and mortality in Kenya are increasing according to recent reports and now number among the top five causes of mortality in the country. The risk factors responsible for this increase in cancer incidences are assumed to be genetic and/or environmental in nature. The environmental factors include exposure to carcinogenic contaminants such aflatoxins (AFs). However, the exact causes of the increase in cancer incidences and prevalence in many developing countries are not fully known. Aflatoxins are known contaminants produced by the common fungi Aspergillus flavus and the closely related Aspergillus parasiticus which grow as moulds in human foods. Aflatoxin B1 (AFB1) is most common in food and is 1000 times more potent when compared with benzo(a)pyrene, the most potent carcinogenic polycyclic aromatic hydrocarbon (PAH). Aflatoxins have therefore drawn a lot of interest in research from food safety and human health point of view. In this chapter, the chemistry, synthesis, identification, toxicology and potential human health risks of AFB1 in Kenya are discussed.

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Section: Analysis and Identification Of Aflatoxin B1mentioning

confidence: 99%

Section: Analysis and Identification Of Aflatoxin B1mentioning

confidence: 99%

Aflatoxin B1: Chemistry, Environmental and Diet Sources and Potential Exposure in Human in Kenya

Lalah¹,

Omwoma²,

Orony³

2020

Aflatoxin B1 Occurrence, Detection and Toxicological Effects

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show abstract

“…Histamine level in food increases during protein degradation. Thus, this compound serves as freshness and sanitary indicator in foods rich in proteins (fish, meat, cheese) as well as sparkling wine and beer because lactic acid fermentation also increases the histamine production [41]. The most common fish implicated in histamine poisoning (known as scombroid poisoning) are tuna, mackerel, herring and sardine, which all have naturally high levels of histidine [42].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the binding performance of histamine‐imprinted microspheres by frontal analysis capillary electrophoresis

et al. 2017

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Frontal analysis capillary electrophoresis was used to evaluate the binding performance of molecularly imprinted microspheres (MIM) toward its template histamine and analogs at pH 7, and compared to the high performance liquid chromatographic method. In both methods, batch binding was employed and the binding parameters were calculated from the measured concentration of unbound amine analytes and afforded comparable histamine equilibrium dissociation constants (K ∼ 0.4 mM). FACE was easily carried out at shorter binding equilibration time (i.e. 30 min) and without the need to separate the microspheres, circumventing laborious and, in the case of the system under study, inefficient sample filtration. It also allowed for competitive binding studies by virtue of its ability to distinctly separate intact microspheres and all tested amines which could not be resolved in HPLC. K 's for nonimprinted (control) microspheres (NIM) from FACE and HPLC were also comparable (∼ 0.6 mM) but at higher histamine concentrations, HPLC gave lower histamine binding. This discrepancy was attributed to inefficient filtration of the batch binding samples prior to HPLC analysis resulting in an over-estimation of the concentration of free histamine brought about by the presence of unfiltered histamine-bound microspheres.

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“…However, immunochemical methods with the use of antigen-antibody reactions belong to selective, sensitive and fast techniques, being relatively inexpensive at the same time. Recently, they are more frequently used for the determination of food components (Leszczynska et al, 2001;Mohammad Reza et al, 2012;Leszczynska et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

Estimation of aflatoxins in peanut or maize by enzyme linked immunosorbent assay

Shukla̽

2016

Bangladesh J Pharmacol

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Aflatoxin are potentially toxic metabolites produced by microscopic fungi when grow on food. Hence, determining content of mycotoxins such as aflatoxin has significant pharmaceutical value. This study reports step-by-step visual demonstration of determination of aflatoxin contents in peanut and maize (corn)-based food samples through employing enzyme linked immunosorbent assay (ELISA). The method is based on direct competitive ELISA in a micro-well format which allows determination of exact amount of aflatoxin in food samples as parts per billion (ppb), and the results are calculated using Neogen's Veratox data reduction software that converts the absorbance values into quantities. This visual experiment will certainly contribute knowledge regarding importance and applicability of the ELISA method for determining aflatoxin content in variety of food samples avoided of analytical issues such as sampling, laborious sample preparation, and critical selection of analytical methods.

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Determination of aflatoxins in food products by the ELISA method

Cited by 34 publications

References 15 publications

Aflatoxin B1: Chemistry, Environmental and Diet Sources and Potential Exposure in Human in Kenya

Aflatoxin B1: Chemistry, Environmental and Diet Sources and Potential Exposure in Human in Kenya

Assessment of the binding performance of histamine‐imprinted microspheres by frontal analysis capillary electrophoresis

Estimation of aflatoxins in peanut or maize by enzyme linked immunosorbent assay

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