Determination of albuterol in human serum by reversed-phase high-performance liquid chromatography with electrochemical detection

Emm, Thomas; Lesko, Lawrence J.; Leslie, James; Perkal, M.B.

doi:10.1016/0378-4347(88)80120-8

Cited by 31 publications

(3 citation statements)

References 9 publications

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“…For pharmacokinetics studies in humans, methods based on a wide range of extraction procedures have been published for the quantification of individual ß-agonists. Salbutamol has been extracted from plasma by solvent extraction, ion-exchange extraction (Lindberg and Jönsson 1982), solid-phase extraction (SPE) (Leferink et al 1982, Emm et al 1988) and ion-pair extraction (Hutchings et al 1983, Miller andGreenblatt 1986). With regards to the detection of salbutamol, several techniques have also been described in the literature, e.g.…”

Section: Introductionmentioning

confidence: 98%

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Montrade¹,

Bizec²,

Monteau³

et al. 1995

Food Additives and Contaminants

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The fate of salbutamol sulphate given orally has been investigated in calves. The urinary excretion rate and the tissue distribution of this beta-agonistic drug were studied by capillary gas chromatography coupled to low resolution mass spectrometry (GC-LRMS) under electron impact (EI) ionization mode, using an hexadeuterated salbutamol analogue as the internal standard. The parent drug and metabolites were extracted via solid phase extraction (SPE) mixed-phase-containing disposable columns and analysed as their trimethylsilyl derivatives. A more efficient clean-up had to be carried out for tissue samples. An acidic precipitation followed by a liquid-liquid extraction were therefore performed before the SPE. Moreover, the problem of tissue digestion was elucidated by means of an ultrasonic probe. Samples were also analysed before and after enzymic hydrolysis using purified beta-glucuronidase and a mixture of beta-glucuronidase and arylsulphatase, to obtain evidence of phase II conjugation mechanisms. Both free salbutamol and conjugated metabolites were detected in urine and tissue samples. Except for liver or kidney, salbutamol was rapidly cleared from most tissues after a withdrawal period. The possible excretion of some phase I metabolites was also investigated, using further analyses under positive chemical ionization LRMS and high resolution mass spectrometry (HRMS).

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Section: Introductionmentioning

confidence: 98%

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Montrade¹,

Bizec²,

Monteau³

et al. 1995

Food Additives and Contaminants

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“…Alternative low cost methods for the measurement of salbutamol and terbutaline have utilized high performance liquid chromatography (HPLC) with electroche-* Author to whom correspondence should be addiessed. mica1 and fluorescence detection (Edholm et al, 1982;Williams et al, 1982;Bergquist and Edholm, 1983;Edholm et al, 1984;Emm et al, 1988;Kurosawa et al, 1984;Ong et uf., 1989). Many of these have been multicolumn methods, often with on-line solid phase extraction.…”

Section: Lntroductionmentioning

confidence: 97%

Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

McCarthy

Atwal

Sykes³

et al. 1993

Biomedical Chromatography

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A method is described for the determination of terbutaline and salbutamol in plasma from patients given maximal therapy for brittle asthma. The analytes were isolated by solid phase extraction on alkali-treated Bond-Elut, unmodified, silica columns and measured by high performance liquid chromatography with fluorescence detection (excitation wavelength 200 nm). The limits of detection for a 1 mL sample containing salbutamol and terbutaline were 1 microgram/L and 2.5 micrograms/L, respectively. The intra-assay precision (CV) for samples containing 25 micrograms/L was 3.6 and 5.0% respectively. This method was applied to the measurement of terbutaline in samples from patients given continuous infusions of the drug to assess whether this treatment might result in toxicity.

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“…Thus, numerous methods have been reported for the analysis of albuterol in biological samples by gas chromatography-mass spectrometry (GC-MS), 12,13 capillary electrophoresis (CE), 14,15 supercritical fluid chromatography (SFC), 16 high-performance thin layer chromatography (HPTLC), 17,18 and high-performance liquid chromatography (HPLC) coupled with either electro-chemical, 6,7,[19][20][21] or fluorescence detection. 5,8,11 However, only a few HPLC methods 6,11,22 are sensitive down to the sub-nanogram/ml levels necessary to monitor the plasma levels produced by the low doses of albuterol MDI therapy.…”

mentioning

confidence: 99%

Determination of the enantiomers of albuterol in human and canine plasma by enantioselective high-performance liquid chromatography on a teicoplanin-based chiral stationary phase

1998

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Determination of albuterol in human serum by reversed-phase high-performance liquid chromatography with electrochemical detection

Cited by 31 publications

References 9 publications

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection

Determination of the enantiomers of albuterol in human and canine plasma by enantioselective high-performance liquid chromatography on a teicoplanin-based chiral stationary phase

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