Determination of alternative pathway of complement activity in mouse serum using rabbit erythrocytes

Dijk, Hans van; Rademaker, P.M.; Willers, J. M. N.

doi:10.1016/0022-1759(80)90091-5

Cited by 45 publications

(17 citation statements)

References 20 publications

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“…Spleen and liver were removed and immediately frozen in liquid nitrogen and stored at -70 "C. After clotting of the blood at 20°C for 1.5 h, serum was separated by centrifugation and subsquently stored at -70°C until use. Determination of the alternative C pathway activity was performed according to the procedure described by Van Dijk et al [18], using rabbit RBC as indicator cells. Alternative C pathway activity was expressed in AP50 U/ml, in which 1 AP50 U causes 50% lysis of the rabbit RBC.…”

Section: Depletion and Immunizationmentioning

confidence: 99%

Complement‐mediated follicular localization of T‐independent type‐2 antigens: the role of marginal zone macrophages revisited

Eertwegh¹,

Laman²,

Schellekens³

et al. 1992

Eur J Immunol

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In this study we demonstrate a hitherto undescribed phenomenon, namely that thymus-independent type-2 antigens (TI-2 Ag) localize in splenic follicles within 1 h after administration. The follicular localization of 2,4,6-trinitrophenyl (TNP)-Ficoll was not antibody mediated. In addition in case of high-dose administration we observed a relatively large amount of TI-2 Ag in marginal zone macrophages. However, after low-dose administration we observed a preferential localization of TNP-Ficoll in the splenic follicles. Detection of TNP-haptenated Ag in cryostat sections of murine spleens was performed with a high-affinity TNP-specific monoclonal antibody conjugated to beta-galactosidase. Within minutes after injection the TI-2 Ag localized in the marginal zone, attached to marginal zone macrophages and B cells. Twenty minutes after injection the Ag was also detected in the follicles and gradually accumulated there until 7 h after injection. Thereafter, the amount of follicular Ag gradually decreased but was still detectable up to 14 days after immunization. The follicular localization of TNP-Ficoll was complement dependent in contrast to the binding to and uptake by marginal zone macrophages. Double staining revealed that Ag was bound by macrophages, B cells and follicular dendritic cells. Haptenated thymus-dependent (TD) Ag localized exclusively in the red pulp macrophages. In vivo macrophage elimination drastically increased the amount of TNP-Ficoll in the follicles, and enhanced the humoral immune response at low doses of Ag. Moreover, complement deprivation of mice abrogated the localization of TI-2 Ag in the follicles, and led to a decreased humoral TI-2 immune response. In conclusion, we demonstrate for the first time that TI-2 Ag localize in follicles. Moreover, the presented results provide further evidence that B cells and follicular localized Ag play an important role in the induction of humoral TI-2 immune responses.

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Section: Depletion and Immunizationmentioning

confidence: 99%

Complement‐mediated follicular localization of T‐independent type‐2 antigens: the role of marginal zone macrophages revisited

Eertwegh¹,

Laman²,

Schellekens³

et al. 1992

Eur J Immunol

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“…The basic principle for the activity determination of CP is based on the hemolysin pre-coated sheep erythrocytes which is hemolyzed by complement (Costabile, 2010). However, AP activity is analyzed with erythrocytes without hemolysin (Van Dijk, Rademaker, & Willers, 1980). The LP activity can also be detected by hemolytic assay (Kuipers, Aerts, Sjöholm, Harmsen, & van Dijk, 2002).…”

Section: Hemolytic Methodsmentioning

confidence: 99%

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Ijaz

et al. 2015

Carbohydrate Polymers

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“…Serum C3 depletion was confirmed by alternative complement pathway activity as described previously [20].…”

Section: Cobra Venom Factor (Cof)mentioning

confidence: 99%

The selective localization of B lymphocytes in the spleen and the role of complement receptors

Kraal¹,

Hoogstraten²,

Klerx³

et al. 1985

Eur J Immunol

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The role of complement receptors on the localization of T and B cells in the spleen of mice was studied using short-term homing experiments in cobra venom factor (CoF)-treated animals. The localization ratio of B and T cells in the spleen of CoF-treated mice decreased significantly compared to control recipients. No changes could be found in the relative distribution of resident T and B cells in the spleen or other lymphoid organs of CoF-treated animals and when their spleen or lymph node cells were transferred, the localization pattern was normal. When cells were incubated in serum prior to transfer a disturbed localization ratio in the spleen of untreated recipients was observed. This was due to a blockade of complement receptors as determined by the inability of the incubated cells to form EAC rosettes. No blockade of EAC rosettes and no changes in localization ratios upon transfer could be observed when the cells were incubated in functionally C3-depleted serum. The results suggest a role for the complement-receptor on B cells in the initial localization in the spleen, whereas no influence upon the selective localization in high endothelial venules-bearing organs was found.

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Determination of alternative pathway of complement activity in mouse serum using rabbit erythrocytes

Cited by 45 publications

References 20 publications

Complement‐mediated follicular localization of T‐independent type‐2 antigens: the role of marginal zone macrophages revisited

Complement‐mediated follicular localization of T‐independent type‐2 antigens: the role of marginal zone macrophages revisited

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

The selective localization of B lymphocytes in the spleen and the role of complement receptors

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