Determination of aluminium using high performance chelation ion chromatography

Abstract

Original PaperDetermination of aluminium using high performance chelation ion chromatographyThe suitability of high performance chelation ion chromatography (HPCIC) using postcolumn reaction for the separation and determination of dissolved aluminium in complex samples was investigated. Use of a chelating ion-exchanger allowed for differentiation between kinetically labile and kinetically stable species of aluminium. Separation through a combination of chelation and cation-exchange was achieved using a 20064.0 mm id column packed with particles of silica functionalised with iminodiacetic acid, with nitric acid -potassium chloride eluents. A temperature anomaly causing a five-fold increase in column efficiency for aluminium is believed to be a result of localised temperature effects in the particular type of instrument used. Postcolumn reagents investigated for the photometric detection included Tiron, Pyrocatechol Violet, Chrome Azurol S, and Eriochromem Cyanine R. The lowest detection limit (2.7 lg/L for a 100 lL sample volume) was achieved using 0.25 mM Eriochrome Cyanine R in 0.2 M hexamine (pH 6.1) with 1 mM cetyltrimethylpyridium bromide (CTAB). The optimised HPCIC system was applied successfully to the quantification of labile aluminium in paper mill process water.

Fast and sensitive determination of aluminium with RP-HPLC using an ultra-short monolithic column

“…Optimal pH for aluminium-lumogallion complex formation is pH 5.5 AE 0.1. 6,9,20,34 Buffers that have been used in other methods include ammonium-acetate (logK Al z 2 35 ), 16,20,21 potassium hydrogen phthalate (logK Al z 3 36 ) 4,5 and MES (no data or evidence of complex formation was obtained 37 ). 6 MES was selected here because it does not co-ordinate with metals (unlike ammoniumacetate and phthalate), is non-hazardous, easy to handle and can be easily purified (see section 2.2).…”

“…5 In environmental chemistry, aluminium is also used as a tracer of dust deposition events and the movement of water bodies in the ocean. [6][7][8][9][10][11] Determination of total aluminium in this wide range of samples has been achieved using a number of methods, mainly spectroscopic, including graphite furnace atomic absorption spectroscopy (GFAAS), 12 flame atomic absorption spectroscopy (FAAS), 13 ultraviolet -visible spectrophotometry (UV-VIS), 14 instrumental neutron activation analysis (INAA), 3 electron capture detection gas chromatography, 15 and inductively coupled plasma mass spectrometry (ICP-MS). 3,16 However, as these methods only determine total aluminium, different methods are required to analyse the various species of aluminium in a sample, such as differentiating between reactive and nonreactive forms.…”

“…They can remove analytes from their sample matrix while simultaneously separating different chemical species of an element from each other. They typically have lower detection limits, require smaller sample volumes and can be used to improve performance of existing techniques, such as GC-GFAAS, 7 sequential injection analysis (SIA) 18,19 and flow injection analysis (FIA) 20,21 with inline concentration columns, high performance chelation ion chromatography (HPCIC) 6,9 and HPLC-MS/MS. 22 It should be noted that SIA and FIA techniques that use inline pre-concentration onto mini-columns (both conventional and monolithic) with an elution program, may be considered as lowpressure liquid-chromatography techniques, such as Victory et al 23 and Pelletier and Lucy.…”

“…Determination of aluminium using chromatography has been achieved using ion chromatography, 25 high performance chelation ion chromatography (HPCIC), 6,9 size exclusion chromatography (SEC) 26 and reversed-phase high-pressure liquid-chromatography (RP-HPLC) of metal chelates. 1,4,5,16 RP-HPLC of metal chelates was selected as it is one of the most sensitive, accurate and efficient methods for determination of aluminium.…”

METHOD FOR RAPID ASSESSMENT OF ALUMINUM TOLERANCE OF PEA (Pisum sativum L.)

“…Eriochrome cyanine R forms a purple colored complex with aluminum and is used to detect and determine aluminum concentrations in chemistry [25], chromatography [26], medicine [27], cytology [28], and ecology [29]. To study the effect of aluminum on plants, including assessment of intra-specific plant aluminum tolerability, this dye was first used in the comparison of aluminum content in different wheat varieties [21] and then in rye and triticale [2].…”