Fast and sensitive determination of aluminium with RP-HPLC using an ultra-short monolithic column

Remenyi, Tomas A.; Nesterenko, Pavel N.; Butler, Edward C. V.; Haddad, Paul R.

doi:10.1039/c1ay05192j

Cited by 2 publications

(3 citation statements)

References 33 publications

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“…Preliminary work (Remenyi et al 2011) proved the co-elution of the seawater matrix with the analyte interfered with fluorescence of the Al-lumogallion complex. Although this is not a problem at higher concentrations (see Giesbrecht 2007), we suspected it may be an issue < 1 nM and therefore included the matrix elimination step.…”

Section: Analysis Of the Prepared Samplementioning

confidence: 99%

“…Eluent B was a 90/10 (v/v) methanol/water mixture, applied to remove the analyte from the column and pass it into the detector. More details of this system can be found in Remenyi et al (2011).…”

Section: Analysis Of the Prepared Samplementioning

confidence: 99%

“…To reduce sample volume requirements and improve the capacity for automation, the method presented by Remenyi et al (2011) was adapted to determine dissolved aluminum at trace levels in seawater. This development provided increased efficiencies in required sample volume, automation, and robustness compared with the most popular techniques currently used.…”

Section: Reversed Phase High Performance Liquid Chromatographic Determentioning

confidence: 99%

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Reversed phase high performance liquid chromatographic determination of dissolved aluminium in open ocean seawater

Remenyi

Nesterenko

Butler

et al. 2012

Limnology & Ocean Methods

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Popular shipboard techniques to determine dissolved aluminium in open ocean seawater have two major disadvantages: relative to HPLC techniques, they require large sample volumes; and, in practice they are are only semi-automated. To address these two issues, the application of a reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of the aluminium in open ocean seawater was developed. With this method, dissolved aluminium in the sample was complexed with lumogallion at pH 5.5 by the addition of a reagent, producing the fluorescent aluminium-lumogallion complex. This complex was concentrated onto a Chromolith ® RP-18e Guard (10 × 4.6 mm) column and eluted with 95% methanol into a fluorescence detector (λ ex = 505 nm, λ em = 574 nm). Sample pre-treatment required 10 h (>100 samples can be prepared simultaneously) and sample analysis required 375 mL of sample per vial analysed, with 10 mL and 2.7 min per injection. Total required sample volume (including rinses) was 1.5 mL. Limit of detection was 0.13±0.05 nM, with precision of 2.7% at 1 nM on SAFe reference samples. Agreement with SAFe reference samples was within 4.6±4.6% (n = 13). The technique is robust, inherently automated and could easily be applied shipboard for open ocean analyses. It provides improvements in sample efficiency, operational robustness and automation relative to popular shipboard techniques, especially when applied to samples with dissolved aluminium concentrations that approach the limit of detection. This project was a contribution to the GEOTRACES program.

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Section: Analysis Of the Prepared Samplementioning

confidence: 99%

Section: Analysis Of the Prepared Samplementioning

confidence: 99%

Section: Reversed Phase High Performance Liquid Chromatographic Determentioning

confidence: 99%

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