Determination of amino acids by micellar EKC: Recent advances in method development and novel applications to different matrices

Iadarola, Paolo; Ferrari, Fábio; Fumagalli, Marco; Viglio, Simona

doi:10.1002/elps.200700662

Cited by 24 publications

(15 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3A). The application to amino acid analysis was recently reviewed [66]. The technique operates at high pH in normal polarity mode (cathode at outlet) where strong EOF is directed toward the cathode.…”

Section: Mode Of Separation and Chiral Recognitionmentioning

confidence: 99%

See 1 more Smart Citation

Separation and sensitive detection of D‐amino acids in biological matrices

Kirschner

Green

2009

J of Separation Science

View full text Add to dashboard Cite

The increase in our understanding of D-amino acid function and distribution in mammals is in many ways a result of the initial development of sensitive enantioselective separation strategies that allow for quantification in real biological samples. This article reviews progress on the development of chiral selective separation and detection of D-amino acids including enzyme-based microbiosensors, GC/MS, HPLC/fluorescence, HPLC/MS-MS, cEKC/fluorescence, and MEKC/fluorescence. Only methods capable of analyzing D-isomers in biological matrices are given here and significant effort is made to highlight approaches that offer speed, resolution, high sensitivity, and versatility.

show abstract

Section: Mode Of Separation and Chiral Recognitionmentioning

confidence: 99%

“…Chiral amino acid separations by CE-LIF has been extensively studied and recently reviewed [66] in part. Many chiral techniques were not optimized for biological samples initially by their investigators.…”

Section: Mode Of Separation and Chiral Recognitionmentioning

confidence: 99%

Separation and sensitive detection of D‐amino acids in biological matrices

Kirschner

Green

2009

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…This makes MEKC very attractive for the differentiation of analytes with similar physico-chemical properties, particularly of amino acids [61]. Therefore, MEKC was employed in this experiment to separate the labeled amino acid neurotransmitters.…”

Section: Optimization Of Ce Separation Conditionsmentioning

confidence: 99%

Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser‐induced fluorescence detection

Deng

Wang

Zhang³

2008

J of Separation Science

View full text Add to dashboard Cite

A CE-LIF detection method has been developed to identify and quantitate six amino acid neurotransmitters including glutamic acid, aspartic acid, gamma-aminobutyric acid, glycine, taurine, and glutamine. N-hydroxysuccinimidyl fluorescein-O-acetate, a fluorescein-based dye, was employed for the derivatization of these neurotransmitters prior to CE-LIF analysis. Different parameters which influenced separation and derivatization were optimized in detail. Under optimum conditions, linearity was achieved within concentration ranges of up to three orders of magnitudes for those analytes with correlation coefficients from 0.9989 to 0.9998. The LODs ranged from 0.06 nM to 0.1 nM, and are thus superior or equivalent to those previously reported in the literature using CE-LIF detection. The proposed method has been successfully applied to the determination of amino acid neurotransmitters in biological samples such as human cerebrospinal fluid and saliva with satisfactory recoveries.

show abstract

“…Among the possible methods used for the enantioselective separation/determination of AAs in different matrices, gas chromatography (GC), micellar electrokinetic chromatography (MEKC), and high-or ultra-performance liquid chromatography (HPLC, UPLC) mostly prevail [6][7][8][9][10][11][12]. Other methods are used less frequently.…”

Section: D/l Amino Acid Ratio As a Tool For The Determination Of Age mentioning

confidence: 99%

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

2016

View full text Add to dashboard Cite

Abstract:Occurrence of D-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain D-amino acids, represents a useful tool in the studies of aging processes or biomarkers in disease/disorder diagnosis in humans. The amount of D-amino acids is usually very low. Therefore, suitable sample pretreatment, often derivatization, and highly selective and sensitive separation methods are essential for D-amino acid analysis in this field. Chromatographic techniques offer appropriate choices for solving these tasks. This review covers the advances in methodology and development of improved instrumental chromatographic methods focused on D,L-amino acid separation and determination. New findings in the area of possible D-amino acid biomarkers are also included.

show abstract

Determination of amino acids by micellar EKC: Recent advances in method development and novel applications to different matrices

Cited by 24 publications

References 115 publications

Separation and sensitive detection of D‐amino acids in biological matrices

Separation and sensitive detection of D‐amino acids in biological matrices

Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laser‐induced fluorescence detection

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

Contact Info

Product

Resources

About