Determination of aminoglycosides in pharmaceutical formulations — I. Thin-layer chromatography

Sekkat, M.; Fabre, H.; Buochberg, M. Simeon De; Mandrou, B.

doi:10.1016/0731-7085(89)80010-x

Cited by 13 publications

(6 citation statements)

References 7 publications

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“…Samples were developed on HPTLC Silica Gel 60 (E. Merck, Gibbstown, NJ) in the lower phase of chloroform : methanol: 29% aqueous ammonia (1:1:1, by vol.) [14]. TLC tanks were equilibrated with the solvent for several hours before each run.…”

Section: Molecular Size Of Metabolite a Molecular Weightmentioning

confidence: 99%

Formation of a toxic metabolite from gentamicin by a hepatic cytosolic fraction

Crann

Huang

McLaren

et al. 1992

Biochemical Pharmacology

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We have demonstrated recently that incubation of the aminoglycoside gentamicin with an hepatic post-mitochondrial fraction produces a compound toxic to sensory cells from the inner ear in short-term culture; in contrast, the parent aminoglycoside was non-toxic in vitro (Huang MY and Schacht J, Biochem Pharmacol 40: R11-R14, 1990). In the present study, we investigated the subcellular distribution of the enzymatic activity and the nature of the metabolite. Isolated outer hair cells from the guinea pig cochlea were used to assay for cytotoxicity. The enzyme(s) responsible for this novel reaction of aminoglycosides was exclusively localized to the cytosolic fraction of guinea pig liver. No activity was detected in nuclear, lysosomal/mitochondrial or microsomal preparations. Furthermore, the toxin-forming enzymatic activity was associated with the high molecular weight fraction of the cytosol and did not require low molecular weight components. Filtration of the toxin through molecular weight cut-off membranes showed a molecular size of approximately 500. This evidence is consistent with the toxin being a gentamicin derivative.

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Section: Molecular Size Of Metabolite a Molecular Weightmentioning

confidence: 99%

Formation of a toxic metabolite from gentamicin by a hepatic cytosolic fraction

Crann

Huang

McLaren

et al. 1992

Biochemical Pharmacology

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“…The residual of kanamycin in animal derived foods is a potential danger to human health [5,6]. At present, many methods have been brought forward for the determination of kanamycin in food residues including capillary electrophoresis (CE) [7], thin-layer chromatography (TLC) [8], high-performance liquid chromatography (HPLC) [9], electrochemical immunosensor [10], gas chromatography-mass spectrometry (LC-MS) [11,12], solid-phase extraction (SPE) [13] and enzyme-linked immunosorbent assay (ELISA) [14]. Although these analysis methods are successful for kanamycin determination, they have many shortcomings involving tedious sample pretreatment, time-consuming and low UV or fluorescence absorption [15,16].…”

Section: Introductionmentioning

confidence: 99%

Imprinted electrochemical sensor based on magnetic multi-walled carbon nanotube for sensitive determination of kanamycin

Long

Zhang

Yang

et al. 2015

Journal of Electroanalytical Chemistry

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“…However, it is very difficult to distinguish amoxicillin from ampicillin and other penicillins using microbiological methods. The method of thin layer chromatography for determination of aminoglycosides was used for manufacturing process and quality control in pure product batches [2]. The final choice of the technique depends upon criteria such as sensitivity, accuracy and selectivity.…”

Section: Introductionmentioning

confidence: 99%