Determination of ammonia nitrogen in solid and liquid high-complex matrices using one-step gas-diffusion microextraction and fluorimetric detection

Valente, Inês M.; Oliveira, Hugo M.; Vaz, Carlos; Ramos, Rui Miguel; Fonseca, António J.M.; Cabrita, Ana R.J.; Rodrigues, José A.

doi:10.1016/j.talanta.2017.01.091

Cited by 25 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The determination of ammonia-N followed the protocol proposed by Valente et al . ( 2017 ). Briefly, 1 g of feces was diluted in 200 mL of ultrapure water (18.2 MΩ cm; Sartorius Arium ® , Goettingen, Germany) and subjected to gas-diffusion microextraction with o -phthalaldehyde labeling for fluorimetric determination in a microplate reader (Synergy HT, Bio-Tek Instruments, Bad Friedrichshall, Germany).…”

Section: Methodsmentioning

confidence: 99%

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Pereira

Pinna

Biagi

et al. 2020

FEMS Microbiology Ecology

View full text Add to dashboard Cite

Selenium is an essential trace element that can modulate the gut microbiome with an impact on host health. The present study aimed to evaluate the effects of organic (selenium-enriched yeast) vs inorganic (sodium selenite) selenium source on fecal end-fermentation products and gut microbiome of puppies from 20 to 52 weeks of age. Alpha and beta diversity of the gut bacterial community were affected by age but not by gender or selenium source. The relative abundance of taxa was differently affected by age, and the DNA concentration of all selected bacterial groups increased with age, although total volatile fatty acids (VFA), acetate, propionate, caproate, and lactate concentrations decreased. Organic selenium was associated with a higher concentration of total VFA, propionate, and butyrate, a higher number of DNA copies of Lactobacillus, and a trend to lower DNA copies of Escherichia coli. Effects on fecal microbiome during growth differed with selenium source. Females had higher fecal end-fermentation products related to protein degradation, whereas males had higher DNA concentration of Bifidobacterium. Organic selenium might be beneficial over inorganic for dog food supplementation due to the positive modulation of the gut microbiome observed in puppies.

show abstract

Section: Methodsmentioning

confidence: 99%

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Pereira

Pinna

Biagi

et al. 2020

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…pH, Ammonia-N, Lactate, and Volatile Fatty Acid Analysis Feces were thawed and diluted to 1:10 in water for pH measurement using a potentiometer Crison,Barcelona,Spain). Ammonia-N was determined in 1 g of feces diluted in 200 ml of ultrapure water (18.2 M cm; Arium R , Sartorius, Göttingen, Germany) and subjected to a gas-diffusion microextraction with o-phthalaldehyde labeling for fluorometric determination in a microplate reader (Synergy HT, BioTek Instruments, Bad Friedrichshall, Germany), according to Valente et al (2017).…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of Zinc Source and Enzyme Addition on the Fecal Microbiota of Dogs

et al. 2021

View full text Add to dashboard Cite

Supplemental zinc from organic sources has been suggested to be more bioavailable than inorganic ones for dog foods. However, the bioavailability of zinc might be affected by dietary constituents such as phytates. The present study aimed to evaluate the effects of two zinc sources (zinc sulfate and zinc proteinate) and the addition of a multi-enzymatic complex from the solid-state fermentation of Aspergillus niger on end-products of fecal fermentation and fecal microbiota of adult Beagles fed a high-phytate diet. The experimental design consisted of three 4 × 4 Latin Squares with a 2 × 2 factorial arrangement of treatments (n = 12 Beagles), with four periods and four diets: zinc sulfate without (IZ) or with (IZ +) enzyme addition, and zinc proteinate without (OZ) or with (OZ +) enzyme addition. Enzyme addition significantly affected Faith’s phylogenetic diversity index, whereas zinc source did not affect either beta or alpha diversity measures. Linear discriminant analysis effect size detected nine taxa as markers for organic zinc, 18 for inorganic source, and none for enzyme addition. However, with the use of a negative binomial generalized linear model, further effects were observed. Organic zinc was associated with a significantly higher abundance of Firmicutes and lower Proteobacteria and Bacteroidetes, although at a genus level, the response varied. The DNA abundance of Clostridium cluster I, Clostridium cluster XIV, Campylobacter spp., Ruminococcaceae, Turicibacter, and Blautia was significantly higher in dogs fed IZ and IZ + diets. Higher abundance of genus Lactobacillus was observed in dogs fed enzyme-supplemented diets. End-products of fecal fermentation were not affected by zinc source or enzymes. An increase in some taxa of the phyla Actinobacteria and Firmicutes was observed in feces of dogs fed organic zinc with enzyme addition but not with inorganic zinc. This study fills a gap in knowledge regarding the effect of zinc source and enzyme addition on the fecal microbiota of dogs. An association of zinc bioavailability and bacteria abundance is suggested, but the implications for the host (dog) are not clear. Further studies are required to unveil the effects of the interaction between zinc sources and enzyme addition on the fecal microbial community.

show abstract

“…Many techniques with high sensitivity and good selectivity have been applied to detect NH 3 , including biosensors, , electrochemical sensors, and optical sensors. , However, these detection systems are expensive and require trained personnel for operation. They are not suitable for popular consumers to evaluate food freshness in real time.…”

Section: Introductionmentioning

confidence: 99%

Hydroxypropyl Guar/Cellulose Nanocrystal Film with Ionic Liquid and Anthocyanin for Real-Time and Visual Detection of NH₃

Meng

Luo

Dong

et al. 2020

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Simple, fast, and accurate detection of food freshness is of considerable significance to ensure food safety. The pH values of foods can be good indicators of their freshness, which can be used for real-time detection of food quality. Herein, we fabricated a pH-sensing film for flexible smart labels based on hydroxypropyl guar (HPG), cellulose nanocrystal (CNC), 1-butyl-3-methylimidazolium chloride (BmimCl), a kind of ionic liquid (IL), and anthocyanin (Anth). We investigated the structure, optical properties, and mechanical strength of the composite films. These films can be used to detect ammonia (NH 3 ) generated from seafood during storage by pH-sensing capability and monitor the real-time freshness of seafood. The HPG/CNC/IL/Anth film exhibited several advantages, such as wide color-change range, easy identification, high sensitivity, good reversibility, excellent stability, and low detection limit. The pH-sensing films can function as flexible smart labels for real-time, visual, and accurate detection of food freshness in cold chain logistics and other fields.

show abstract

Determination of ammonia nitrogen in solid and liquid high-complex matrices using one-step gas-diffusion microextraction and fluorimetric detection

Cited by 25 publications

References 40 publications

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Effects of Zinc Source and Enzyme Addition on the Fecal Microbiota of Dogs

Hydroxypropyl Guar/Cellulose Nanocrystal Film with Ionic Liquid and Anthocyanin for Real-Time and Visual Detection of NH₃

Contact Info

Product

Resources

About

Determination of ammonia nitrogen in solid and liquid high-complex matrices using one-step gas-diffusion microextraction and fluorimetric detection

Cited by 25 publications

References 40 publications

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies

Effects of Zinc Source and Enzyme Addition on the Fecal Microbiota of Dogs

Hydroxypropyl Guar/Cellulose Nanocrystal Film with Ionic Liquid and Anthocyanin for Real-Time and Visual Detection of NH3

Contact Info

Product

Resources

About

Hydroxypropyl Guar/Cellulose Nanocrystal Film with Ionic Liquid and Anthocyanin for Real-Time and Visual Detection of NH₃