Determination of ampicillin sodium using the cupric oxide nanoparticles–luminol–H₂O₂ chemiluminescence reaction

Abstract

A simple and sensitive chemiluminescence (CL) method has been developed for the determination of ampicillin sodium at submicromolar levels. The method is based on the inhibitory effect of ampicillin sodium on the cupric oxide nanoparticles (CuO NPs)-luminol-H2 O2 CL reaction. Experimental parameters affecting CL inhibition including concentrations of CuO NPs, luminol, H2 O2 and NaOH were optimized. Under optimum conditions, the calibration plot was linear in the analyte concentration range 4.0 × 10(-7) -4.0 × 10(-6) mol/L. The limit of detection was 2.6 × 10(-7) mol/L and the relative standard deviation (RSD) for six replicate determinations of 1 × 10(-6) mol/L ampicillin sodium was 4.71%. Also, X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis were employed to characterize the CuO NPs. The utility of the proposed method was demonstrated by determining ampicillin sodium in pharmaceutical preparation.

A novel surface molecularly imprinted polymer as the solid-phase extraction adsorbent for the selective determination of ampicillin sodium in milk and blood samples

“…Several analytical methods have been reported to determine the levels of BLAs in environmental water and foods, such as capillary electrophoresis (CE) [5] , [6] , [7] , chemiluminescence [8] , [9] and liquid chromatography (LC) [10] , [11] . Because of the interference of complex matrix in samples, these analytical methods require sample preparation to obtain the desired sensitivity before chromatographic separation and determination, including extraction, purification and enrichment.…”

Enhanced luminol–O2 chemiluminescence reaction by CuO nanoparticles as oxidase mimics and its application for determination of ceftazidime

“…25,26 The application realm of the system was then further expanded by other researchers. [27][28][29][30][31][32] However, to the best of our knowledge, the oxidase-like activity of CuO NPs, a discovered 33 but less known property of CuO NPs, has somehow been neglected and has not yet been incorporated in analytical applications. It should be noted that the oxidase-like activity of V 2 O 5 nanowires 34 and CoFe 2 O 4 NPs 35 toward the luminol-O 2 CL reaction has also been studied.…”

Cupric oxide nanoparticles‐enhanced chemiluminescence method for measurement of β‐lactam antibiotics

“…This phenomenon was attributed to the peroxidase‐mimic activity of CuO NPs. Using the CuO NPs‐enhanced CL system, some innovative approaches for measurement of species such as hydrogen peroxide, glucose , and cholesterol and some pharmaceuticals including ampicillin sodium , and vancomycin have been made. Therefore, we conducted some preliminary experiments on the CL spectrum of the luminol–H 2 O 2 CL reaction (Fig.…”

“…In this regard, hydroxyl and superoxide radicals that are produced by radical decomposition of H 2 O 2 play critical roles in the CL system. However, H 2 O 2 is also a powerful • OH scavenger . It is possible that the decrease in CL sensitivity for drugs under study at H 2 O 2 concentrations >0.1 and 5 µmol/L, for amoxicillin and cefazolin sodium respectively, may be because H 2 O 2 radical scavenging counteracts its positive role as the radical source in the reaction under these conditions.…”

“…Therefore, incorporation of various nanomaterials (NMs) to CL detection systems could be an appropriate answer to these requirements, high sensitivity and selectivity, in which NMs could act as enhancers, biomolecule carriers and separation tools in the CL reactions . In this way, several NMs including magnetic , gold , silver and cupric oxide nanoparticles, among others with redox catalytic properties, have been exploited as catalysers to enhance CL reactions. For example, it has been found that CuO NPs can increase 400‐fold the light intensity emitted from the luminol–H 2 O 2 CL system based on their peroxidase‐like activity .…”