Determination of anabolic steroids in muscle tissue by liquid chromatography–tandem mass spectrometry

Ultra-high performance liquid chromatography–tandem mass spectrometry in high-throughput confirmation and quantification of 34 anabolic steroids in bovine muscle

“…These high S/N values provide evidence that detection and confirmation is surely possible at or below the CCˇvalues. Compared to values reported in literature for a smaller number of analytes [18][19][20][21][22], the calculated CC˛and CCˇvalues from this study are equal (in most cases) indicating high sensitivity of the reported methodology. In some cases higher values are reported but this may be explained by the higher ALs applied for these compounds, in line with the Belgian National Reference Laboratory's recommendations and the fact that the signal relative standard deviation ((R.S.D.)…”

“…1). This gradient allowed quantitative determination of a much higher number of steroids in meat than so far reported in literature [18][19][20][21][22].…”

“…APCI was selected as ionization source since it provided higher detection sensitivities for the majority of steroidal compounds as compared to ESI. Indeed, it has been reported in literature that APCI results in higher signals for multiresidue analysis of hormones [18]. Next, the MS/MS fragmentation conditions were investigated and collision energies and S-lens voltages were optimized for each individual compound and/or transition (Table 1).…”

“…10.006 other matrices. Several methods on the determination of anabolic steroids in muscular tissue of animal origin have been reported [18][19][20][21][22], monitoring a selection of the existing anabolic steroids (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), sometimes in combination with corticosteroids.…”

A label-free detection of diethylstilbestrol based on molecularly imprinted polymer-coated upconversion nanoparticles obtained by surface grafting

“…[7][8][9] Various methods for the detection of diethylstilbestrol in food are currently available. Gas/liquid chromatography-tandem mass spectrometry [10][11][12] and immunoassay analytical methods 13,14 are sensitive and accurate, but they require complicated sample treatment and are time-consuming. Colloidal-gold strip 15,16 is fast and easy to operate, but the immune antibody is difficult to produce and may lose biological activity upon external treatment.…”