Determination of Antibacterial Activity of Film Coatings against Four Clinically Relevant Bacterial Strains

Abstract

Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa,and Staphylococcus aureus were incubated on titania mesostructured thin film coatings for 24 h. Then, cell viability was studied considering three methods: counting of the number of colony forming units (CFU), live/dead staining, and quantification of extracellular DNA in suspension. Firstly, bacterial count was determined by the standard plate-count technique. Secondly, bacteria membrane integrity was evaluated by utilization of two fluorescent dyes, which allow distinction between live (membrane intact) and dead (membrane disrupted) bacteria. Lastly, extracellular DNA was quantified by spectrophotometry. In this manner, the three aforementioned techniques enabled the study of bacterial viability by qualitative and quantitative analyses.

Antifouling Property of Cu2O-Free Self-Polishing Antifouling Coatings Based on Amide Derivatives Inspired by Capsaicin

“…The antibacterial activities of the ADIC-CSAC coatings against two types of bacteria were examined using the colony count method. − E. coli and S.…”

Degradable Green Polymers, Green Nanopolymers and Green Nanocomposites Derived from Natural Systems: Statistics and Headways

“…Nanopolymer-based green nanomaterials have been applied in films/coatings [142]- [144]. Various combinations have been reported for the multi-layer films/coatingsderived packaging materials [145].…”

Degradable Green Polymers, Green Nanopolymers and Green Nanocomposites Derived from Natural Systems: Statistics and Headways

“…Nanopolymer-based green nanomaterials have been applied in films/coatings [142]- [144]. Various combinations have been reported for the multi-layer films/coatingsderived packaging materials [145].…”