Determination of antibiotics such as macrolides, ionophores and tiamulin in liquid manure by HPLC–MS/MS

Schlüsener, Michael P.; Bester, Kai; Spiteller, Michael

doi:10.1007/s00216-003-1838-9

Cited by 112 publications

(53 citation statements)

References 25 publications

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“…Some of them are conducted by gas chromatography, HPLC and HPLC-MS (Markus & Scherma, 1993a;Chen et al, 2006). The reported LOD of these methods ranged from 0.0014 ppm for HPLC-MS (Schlusener et al, 2003) and 10 ppm for HPLC (Markus & Scherma, 1993b). The sensitivity and the specificity by GC (Markus & Scherma, 1993c) and traditional HPLC is lower than those of LC-MS/MS analysis.…”

Section: Discussionmentioning

confidence: 99%

Validation of an analytical method for determination of 8-alpha-hydroxymutilin

Yankovska¹,

Stoilova²,

Peycheva³

et al. 2015

BJVM

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The objectives of this study were to evaluate the concentration of 8-alfa-hydroxymutilin (8α-HM) as marker residue for tiamulin (pleuromutilins group), to verify and validate an analytical method for the quantitative determination of 8-alpha-hydroxymutilin in porcine tissues (muscle and liver). Quantification of (8α-HM) was performed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method was used to determine the residue of 8α-HM in porcine tissue after administration of Rodotet premix. In order to assess the residues in porcine tissues, an analytical method for 8-alpha-hydroxy-mutilin was verificated and validated in line with accordance of Commission Decision 2002/657/EC and VICH GL49.

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Section: Discussionmentioning

confidence: 99%

Validation of an analytical method for determination of 8-alpha-hydroxymutilin

Yankovska¹,

Stoilova²,

Peycheva³

et al. 2015

BJVM

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“…68 Apesar da predominância do uso de ESI na análise de macrolídeos por LC-MS, a APCI tem sido usada em algumas aplicações e demonstrado vantagens com relação à redução do efeito matriz. [70][71][72] Especialmente quando se trata de fontes ESI, o efeito matriz deve ser considerado em análises quantitativas por LC-MS. Os componentes da matriz podem tanto melhorar como suprimir os sinais dos analitos, e seu efeito pode variar de amostra para amostra. O efeito matriz pode ser estimado comparando-se o sinal dos analitos em solvente com seu respectivo sinal na presença da matriz, através da adição de seus padrões analíticos no extrato, após todo o processo de tratamento de amostra.…”

Section: Ionizaçãounclassified

Aspectos analíticos e regulatórios na determinação de resíduos de macrolídeos em alimentos de origem animal por cromatografia líquida associada à espectrometria de massas

Sismotto¹,

Paschoal²,

Reyes³

2013

Quím. Nova

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Recebido em 25/7/12; aceito em 17/10/12; publicado na web em 20/2/13 ANALYTICAL AND REGULATORY ASPECTS FOR DETERMINATION OF MACROLIDE RESIDUES IN ANIMAL-DERIVED FOODS BY LIQUID CHROMATOGRAPHY ASSOCIATED WITH MASS SPECTROMETRY. This is an overview of LC-MS techniques applied for macrolide determination in food, including sample preparation and method validation, as well as the policies adopted by international agencies regarding their presence in food. Techniques for the analysis of macrolides in food normally include solid phase or liquid-liquid extraction followed by HPLC. UHPLC presents advantages in running time, detectability and solvent consumption. Triple-quadrupoles are the most common analyzers in instruments used for the determination of contaminants in food, but time-of-flight and ion-trap spectrometers have been successfully applied for analyses focusing on the investigation of structural formula or the presence of degradation products.Keywords: macrolides; liquid chromatography; mass spectrometry. INTRODUÇÃOOs antimicrobianos são compostos que inibem o crescimento de determinados microrganismos, podendo ser, de modo geral, utilizados na produção animal para tratar enfermidades (aplicação terapêutica), prevenir contra enfermidades causadas pela presença de organismos patogênicos (aplicação profilática) e melhorar a taxa de crescimento e/ou conversão alimentar (utilizados como promotores de crescimento). Uma grande variedade de substâncias antimicrobianas é usada na produção animal em todo o mundo. A presença de resíduos dessas substâncias em produtos alimentícios é uma questão de grande interesse dos órgãos governamentais responsáveis pela saúde pública e das indústrias de alimentos, pois representa riscos à saúde dos consumidores, ressaltando-se a seleção de formas resistentes de microrganismos, a carcinogenicidade, mutagenicidade, teratogenicidade e a hipersensibilidade. [1][2][3][4][5][6] Sendo o Brasil um país de expressiva atividade pecuária no cenário mundial, é fundamental que estejam em vigor programas eficientes que garantam a qualidade de alimentos desta origem. Para atender a essa demanda, é necessário o desenvolvimento de métodos analíticos confiáveis que possibilitem a determinação de medicamentos veterinários em níveis residuais (na ordem de ng g -1 ), haja vista que são substâncias potencialmente tóxicas para a saúde humana e para o meio ambiente.Segundo Mellon et al., 7 estima-se que nos Estados Unidos cerca de 70% da produção anual de antimicrobianos (considerando um total de 16000 t) tenham sido utilizadas na pecuária com fins não terapêuticos.Dentre as diferentes classes de antimicrobianos existentes, destacam-se os macrolídeos por serem altamente potentes contra uma grande variedade de bactérias Gram-positivas e Gram-negativas. A Organização Mundial para a Saúde Animal (Office International dés Épizoties -OIE) considera alguns macrolídeos, como a eritromicina, espiramicina, tilmicosina e tilosina, como criticamente importantes na pecuária de bovinos, suínos e aves, já que existem p...

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“…The presence of these compounds in the environment could potentially affect many aspects of ecosystem function including alteration of bacterial populations leading to nutrient cycle impacts, potential adverse effects to aquatic and non-target organisms, and possibly influence human health (10,11). One environmental entry point of antibiotics is through land application of manure (7,12,13). Antibiotics are administered to livestock and poultry for treatment of infections as well as for disease prevention, growth promotion, and feed efficiency (14,15).…”

Section: Introductionmentioning

confidence: 99%

Fate of Erythromycin in Sediment-Containing Surface Water Microcosms: How Does Aged Erythromycin in Sediment Influence Bioavailability?

Jessick

Coats

2013

ACS Symposium Series

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The detection of antibiotics in water and sediment systems is of concern due to the potential adverse effects which could be associated with their environmental fate. The central aim of this study was to evaluate the fate of erythromycin in microcosms consisting of pond water and submerged pond sediment. The first study examined the dissipation of erythromycin from spiked water and total recovery of [14C]-erythromycin from water and sediment within microcosms ranged between 90.1% and 48% throughout the 63-day study. Erythromycin was reduced in surface water of sediment-containing systems by day 7, which corresponded to an increase of eryrthromycin detected in sediment. In the second study the availability of aged erythromycin was evaluated by incubating sediment with and without a manure amendment with [14C]-erythromycin for 0, 1, 3, or 8 weeks; followed by assessing movement and availability of erythromycin in sediment microcosms after 1, 3, 7, and 14 days. Results indicated differences in residues from aged sediment, with and without manure additions, in extractable residues at day 7 and 14. The addition of manure resulted in greater extractable erythromycin from aged sediments than from sediments without manure. There was a greater release of erythromycin to the water overlying the manure-treated sediments with fresh and 1 week aged sediment than the unamended sediment after 1 and 2 weeks. The results from this experiment demonstrate the ability of manure to influence the fate of erythromycin in environmental matrices. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. The detection of antibiotics in water and sediment systems is of concern due to the potential adverse effects which could be associated with their environmental fate. The central aim of this study was to evaluate the fate of erythromycin in microcosms consisting of pond water and submerged pond sediment. The first study examined the dissipation of erythromycin from spiked water and total recovery of [ 14 C]-erythromycin from water and sediment within microcosms ranged between 90.1% and 48% throughout the 63-day study. Erythromycin was reduced in surface water of sediment-containing systems by day 7, which corresponded to an increase of eryrthromycin detected in sediment. In the second study the availability of aged erythromycin was evaluated by incubating sediment with and without a manure amendment with [ 14 C]-erythromycin for 0, 1, 3, or 8 weeks; followed by assessing movement and availability of erythromycin in sediment microcosms after 1, 3, 7, and 14 days. Results indicated differences in residues from aged sediment, with and without manure additions, in extractable residues at day 7 and 14. The addition of manure resulted in greater extractable erythromycin from aged sediments than from sediments without manure. There was a greater release of erythromycin to the water overlying the manure-treated

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Determination of antibiotics such as macrolides, ionophores and tiamulin in liquid manure by HPLC–MS/MS

Cited by 112 publications

References 25 publications

Validation of an analytical method for determination of 8-alpha-hydroxymutilin

Validation of an analytical method for determination of 8-alpha-hydroxymutilin

Aspectos analíticos e regulatórios na determinação de resíduos de macrolídeos em alimentos de origem animal por cromatografia líquida associada à espectrometria de massas

Fate of Erythromycin in Sediment-Containing Surface Water Microcosms: How Does Aged Erythromycin in Sediment Influence Bioavailability?

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