Determination of anticonvulsants in human plasma using SPME in a heated interface coupled online to liquid chromatography (SPME-LC)

Abstract

A simple and sensitive method using solid phase microextraction (SPME) and liquid chromatography (LC) with heated online desorption (SPME-LC) was developed and validated to analyze anticonvulsants (AEDs) in human plasma samples. A heated lab-made interface chamber was used in the desorption procedure, which allowed the transference of the whole extracted sample. The SPME conditions were optimized by applying an experimental design. Important factors are discussed such as fiber coating types, pH, extraction time and desorption conditions. The drugs were analyzed by LC, using a C18 column (150 mm  4.6 mm  5 mm); and 50 mmol L À1, pH ¼ 5.50 ammonium acetate buffer : acetonitrile : methanol (55 : 22 : 23 v/v) as the mobile phase with a flow rate of 0.8 mL min À1 . The suggested method presented precision (intra-assay and inter-assay), linearity and limit of quantification (LOQ) all adequate for the therapeutic drug monitoring (TDM) of AEDs in plasma.

Development of on-line molecularly imprinted solid phase extraction-liquid chromatography-mass spectrometry for triazine analysis in corn samples

“…13 According to the principles of green chemistry, the miniaturization of sample preparation techniques has been highlighted due to its low consumption of samples, solvents and reagents. A variety of methods, such as solid phase extraction (SPE), 14,15 solid-phase microextraction (SPME), 16 microextraction by packed sorbent (MEPS), 17 stir bar sorptive extraction (SBSE), 18 bar adsorptive microextraction (BAmE), 19 liquid-phase microextraction (LPME) 20 and dispersive liquid-liquid microextraction (DLLME) 21 can be applied for the preconcentration and clean-up of the analytes in different samples. Allied to the miniaturization advantages, the automation of analyses has showed to be a reliable approach for the avoidance of a multistep and time-consuming sample preparation.…”

Application of sol–gel based molecularly imprinted xerogel for on-line capillary microextraction of fentanyl from urine and plasma samples

“…Nowadays, solid phase microextraction (SPME) is regarded as a rapid, solvent free and economical technique which is an alternative to traditional extraction techniques such as LLE and SPE. [12][13][14][15][16][17] To enhance the extraction selectivity, molecularly imprinted polymers (MIPs), are currently being used by many researchers. Despite many advantages, molecularly imprinting technology still needs some more development to overcome few limitations such as template leakage, poor accessibility of the binding sites, low binding capacity and non-specic binding.…”