Multiobjective optimization of microemulsion- thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions

Abou-Taleb, Noura H.; El-Sherbiny, Dina T.; El‐Enany, N.; El‐Subbagh, Hussein I.

doi:10.1016/j.chroma.2020.460945

Cited by 7 publications

(1 citation statement)

References 35 publications

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“…Chromatographic methods of analysis are widely used to determine lamotrigine in biological fluids. Methods of analysis of high-performance liquid chromatography (HPLC) (in plasma [2,3], in serum [4,5], in rat plasma and brain [6]), thin-layer chromatography (TLC) [7] and gas chromatography (GC) [8] for the determination of lamotrigine in biological samples are described, as well as methods of capillary electrophoresis [9], HPLC and GC [10], 1 H-NMR determination [11], TLC and planar chromatography in pharmaceutical forms. Information is provided on some spectrophotometric techniques for the determination of lamotrigine in the UV-(in pure form and in dosage forms [12], a first-order derivative UV method [13], spectrophotometry in the UV region after procedure of extraction [14]) and visible regions of the spectrum (using sulfophthalein dyes [15,16] or 4-(dimethylamino)benzaldehyde in acid medium [17] to give colouring products).…”

Section: Introductionmentioning

confidence: 99%

New, simple and express determination of lamotrigine in tablets by using diazole red 2J

Miedviedieva

Vasyuk

Korzhova

et al. 2022

SR: PS

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Epilepsy is considered one of the most common chronic neurological diseases among humans. Lamotrigine is an effective new-generation anticonvulsant, which is widely introduced to the pharmaceutical market by various drug manufacturers. That is why the urgent aim for pharmaceutical analysis is developing of high precision, valid, accessible and quantitative methods for lamotrigine in pharmaceuticals. The aim of the work is to study the optimal conditions for the reaction between lamotrigine and diazole red 2J, to define the coefficients of stoichiometric relationships in the “pharmaceutical substance – reagent” system and to develop a valid, sensitive and easy-to-follow technique for the quantitative determination of lamotrigine in pharmaceutical forms. Material and methods. As reagent and solvent, diazole red 2J of AR grade and acetone of AR grade were used. Analytical equipment: Specord 200 spectrophotometer, ABT-120-5DM electronic scales, Elmasonic E 60H ultrasonic bath and measuring glassware of A class. Results. A new spectrophotometric method for the quantitative determination of lamotrigine in four pharmaceutical formulations based on interaction with diazole red 2J in acetone medium has been developed. The proposed method is valid according to such validation characteristics as linearity, precision, intra-laboratory precision, accuracy, application range and robustness. The subordination to Behr's law is in the range of concentrations of 2.20–3.36 mg/100 ml. The LOD and LOQ values based on the values of the calibration line were 0.00450 % and 0.0138 % respectively. It was found that the studied coloured solutions are stable for at least 60 min and fluctuations in the amount of added red diazole 2J solution within ±10 % do not significantly affect the value of optical density. The coefficients of stoichiometric ratios between the components of the “lamotrigine – diazole red 2J” reaction mixture were defined by three methods and are 1:1. The predicted complete uncertainty of the results of analysis for quantitative determination of lamotrigine in the pharmaceutical form (2.2 %) does not exceed the maximum allowable uncertainty for the technique (3.2 %) and meets the SPhU requirements. Conclusions. According to the experimental data, the technique can be correctly reproduced and it is suitable for using in laboratories of the State Inspection for Quality Control of Medicines and QCD of the chemicopharmaceutical enterprises

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