Determination of Antioxidant, Antimicrobial Properties with Evaluation of Biochemicals and Phytochemicals Present in Oscillatoria limosa of District Jamshoro, Pakistan

Abstract

Oscillatoria limosa is a well-known member of blue-green algae, usually found in open water reservoirs. Ecologically it plays very important roles, like releasing Oxygen and being a supportive alternative source of food for aqua fauna. In research, it is being investigated as a medicinal organism for cancer and infectious diseases. In the current study, we have determined the medicinal and ecological importance of Oscillatoria limosa particularly found in the vicinity of district Jamshoro 76080, Sindh, Pakistan. For this purpose, four different solvent extracts of 20% (w/v) dried powder of organism were used to determine the presence of bioactive compounds, phytochemicals, antimicrobial activities, and antioxidant properties through previously well-reported methods. The obtained results of this study prove the presence of phenolic acid, flavonoids, total proteins, total sugar, reducing sugar and one free amino acid, and phytochemicals like alkaloids, phytosterol, tannin, terpenoids, glycosides, and saponin in samples. In this study, remarkable antioxidant properties ranging from 0.248 to 1.080 mg ml -1 were observed in all the samples. The antibacterial activities against S. aureus, A. tumefaciens, K. aureus, E.coli, and P.aeruginosa, and antifungal activities against A. niger, P. notatum, and Rhizopus were observed, which proves it a good antimicrobial organism. It may be concluded from this study that Oscillatoria limosa of local vicinity is a potential organism of interest for biotechnological and pharmaceutical industries as an antitumor, antimutagen, free radical scavenger, and possess antimicrobial properties against various kind of bacteria and fungi.

Bacterial Lipopeptides Are Effective against Pear Fire Blight

“…TLC analysis revealed the presence of lipopeptides in the methanol extract, as evidenced by the formation of a blue-purple substance from P. megaterium H1, with corresponding RF values of 0.231, 0.430, 0.569, and 0.641, while for B. subtilis I2, they were 0.251, 0.271, 0.359, 0.541, and 0.630. These RF values imply that the methanol extract contains lipopeptides (surfactins, iturins, bacillomycin D, and fengycins), consistent with prior research [11,42,55].…”

Determination of Phytochemicals, Antimicrobial, Antioxidant and Allelopathic Effects of Fagonia cretica L., collected from Jamshoro, Pakistan

“…Samples of 10% extracts were extracted by our previously described method (Tunio et al, 2022). Briefly, 2.0g of powder was dissolved in 10ml solvent (water, 70% methanol, and 70% ethanol) and centrifuged at 6000rpm for 30 minutes.…”

“…The total antioxidant activity of different extracts of the plant was observed, as described in previous work Tunio et al, 2022). Briefly, 0.2ml sample and 2ml of reagent solution were mixed and incubated in an Eppendorf tube for 90 minutes at 95 ºC.…”

“…Total phenolics from all extracts were estimated using the Folin-Ciocalteu method (Tunio et al, 2022). Total flavonoids were estimated on a spectrophotometer by the aluminum chloride method of Djeridane et al (Djeridane et al, 2006).…”

Tocopherol Content of Euglena sp. Isolated from Yogyakarta under Glucose and Ethanol Mixture Treatment

“…In addition, these microalgae can produce secondary metabolites utilized as antioxidants (Zulkarnain et al, 2020). Previous research by Tunio et al (2022) also reported that cyanobacteria Oscilatoria limosa produced some bioactive compounds including phenolic acid, flavonoid, proteins, amino acid, and sugar.…”