The aim of this study was to explore the effectiveness of different parts of J. curcas plant against some selected human pathogens as antimicrobial agent which are known to cause diseases and to check antioxidant and phytochemicals from different plant sections of J. curcas . Plant extracts were analyzed by quantification of antimicrobial and phytochemical compounds. This study reveals that 20% ethanol stem extract of J. curcas showed maximum antibacterial activity (40 ± 0.0 mm) against Klebsiella pneumonia . Water extract of root of J. curcas also inhibited E. coli (35.25 ± 0.35 mm). The growth of K. pneumonia and Agrobacterium tumifaciens were also ceased when ethanol extract of J. curcas root applied to check their potential as antimicrobial agent. The results also revealed that fungal species, Aspergillus niger, and Pencillium notatum noted the maximum antifungal activity in ethanol extract of flower and methanol extract of root (38.5 ± 0.7 mm) and (27.25 ± 0.35 mm) respectively. Phytochemicals and many secondary metabolites were present in J. curcas extracts such as alkaloids, steroids, tannins, glycosides, flavonoids, saponins, courmerin, and phenolic compounds. It also showed the highest density of color in the different parts of plant extract of J. curcas . Similarly, biochemical primary metabolites were observed at maximum amount of biochemical in different parts of J. curcas, and correlated with antimicrobial activity. The study concluded that J. curcas has great potential as antibacterial agent and cure various human pathogens.

Detection of heavy metals (HMs) content from Keenjhar Lake water was carried out monthly from January to December, 2003. Zinc, chromium, copper, iron, manganese, nickel and cadmium were analyzed by dual mode of analytical methods flame atomic absorption spectrometry and electrothermal atomic absorption spectrometry (FAAS and ETAAS) by multi element standard solution. The concentrations of zinc, chromium, copper, iron, manganese Nickel and cadmium were 1.4 to 104.3 µgL-, respectively. Zn 2+ , Cr + , Cu 2+ , Fe 2+ and Mn 2+ concentrations were recorded lower than those of permissible limits, whereas Ni + and Cd + were higher than those of permissible limits recommended by WHO (2004).

Background: Primary amoebic meningoencephalitis (PAM) is an acute and fulminant CNS infection caused by Naegleria fowleri. Recreational activities and ritual ablution with contaminated warm fresh water are the main reason of PAM. Pakistan ranked the second most affected country, where most of the PAM incidences were reported from Karachi, Pakistan. Methods: In May, 2019, a 28-yr-old suspected PAM patient came to the Imam Zain-Ul-Abdin Hospital, Karachi. Biochemical and cytological investigations of patient`s CSF were carried out at Karachi Diagnostic Center and Molecular Biology Lab. Sequencing of Naegleria sp. specific (ITS) primer-based amplicons was performed from both patient`s CSF and water samples followed by multiple sequence alignment and phylogenetic studies. Results: Biochemical and cytological investigations of patient`s CSF showed 5 mg/dl glucose, 240 mg/dl total protein and 2260/mm3 TLC suggesting acute meningoencephalitis. PCR-based analyses of patient`s CSF and his residential tap water samples using Naegleria sp. specific (ITS) and N. fowleri specific primers revealed the presence of N. fowleri DNA. Nucleotide sequences of ITS primer-based amplicons from both patient`s CSF and water samples were submitted in GenBank under the accession numbers MT726981.1 and MT726226.1, respectively. According to phylogenetic analysis, N. fowleri isolate from Pakistan has shown the least node age of seven. Conclusion: Here, for the very first time in Pakistan, N. fowleri genotype has been identified as type-2. Phylogenetic analysis showed that N. fowleri isolate from Pakistan is among the latest descendants, i.e., evolved later in life.

Oscillatoria limosa is a well-known member of blue-green algae, usually found in open water reservoirs. Ecologically it plays very important roles, like releasing Oxygen and being a supportive alternative source of food for aqua fauna. In research, it is being investigated as a medicinal organism for cancer and infectious diseases. In the current study, we have determined the medicinal and ecological importance of Oscillatoria limosa particularly found in the vicinity of district Jamshoro 76080, Sindh, Pakistan. For this purpose, four different solvent extracts of 20% (w/v) dried powder of organism were used to determine the presence of bioactive compounds, phytochemicals, antimicrobial activities, and antioxidant properties through previously well-reported methods. The obtained results of this study prove the presence of phenolic acid, flavonoids, total proteins, total sugar, reducing sugar and one free amino acid, and phytochemicals like alkaloids, phytosterol, tannin, terpenoids, glycosides, and saponin in samples. In this study, remarkable antioxidant properties ranging from 0.248 to 1.080 mg ml -1 were observed in all the samples. The antibacterial activities against S. aureus, A. tumefaciens, K. aureus, E.coli, and P.aeruginosa, and antifungal activities against A. niger, P. notatum, and Rhizopus were observed, which proves it a good antimicrobial organism. It may be concluded from this study that Oscillatoria limosa of local vicinity is a potential organism of interest for biotechnological and pharmaceutical industries as an antitumor, antimutagen, free radical scavenger, and possess antimicrobial properties against various kind of bacteria and fungi.

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