2002
DOI: 10.1007/s00216-002-1638-7
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Determination of azadirachtin and fatty acid methyl esters of Azadirachta indica seeds by HPLC and GLC

Abstract: A simple and economical method has been developed to estimate the azadirachtin content and fatty acid composition of neem kernels. Neem kernels are crushed and soaked overnight in ethanol. The extract obtained is analysed by HPLC after filtering through a 0.22 micro m membrane. The peaks are separated using acetonitrile-water (40:60) 1 mL min(-1) as the mobile phase on an RP-18 column and monitored at 214 nm. For the determination of fatty acid composition, the fatty acids are directly transmethylated in the k… Show more

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Cited by 45 publications
(33 citation statements)
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“…However, a HPLC analysis conducted according to the protocol of Kaushik [43] did not evidence additional compounds present in significant amounts in NA, but revealed that the azadirachtin A content of AZA was about half the amount declared by the manufacturer, which could well explain the difference in transmission blocking effect observed with the two products.…”
Section: Discussionmentioning
confidence: 99%
“…However, a HPLC analysis conducted according to the protocol of Kaushik [43] did not evidence additional compounds present in significant amounts in NA, but revealed that the azadirachtin A content of AZA was about half the amount declared by the manufacturer, which could well explain the difference in transmission blocking effect observed with the two products.…”
Section: Discussionmentioning
confidence: 99%
“…There are over 50 different bioactive compounds (terpenoids and others) of these aqueous extracts, but the major components of neem and cinnamon are limonoids such as azadirachtins (AZ) (Huang et al, 1996;Nakatani et al, 1998;Kaushik, 2002;D'Ambrosio & Guerriero, 2002). The general biological action of these extracts is the induction of lipid peroxidation, generation of antiproliferative and antioxidant effects and detoxication of enzymes (Akudugu et al, 2001;Kumar et al, 2006).…”
Section: Discussionmentioning
confidence: 99%
“…Kaushik reported that the losses of azadirachtin were about 8-33% by hexane extraction of neem seed [41]. The existence of equilibrium between hexane-oil-limonoids limits the complete separation of azadirachtin from oil, thus result in azadirachtin losses.…”
Section: Comparison Of Pre-treatment Methodsmentioning
confidence: 98%