Determination of Benzalkonium Chloride by Chemical Ionization Mass Spectroscopy

High Performance Liquid Chromatography of Benzalkonium Chlorides-Variation in Commercial Preparations

“…T h e area under the peak for each homologue responded linearly with concentration within the range examined. Variation in the proportion of homologues is possible from different sources of benzalkonium chlorides (5,10,12), therefore commercial 0~02",w/v, 509,w/v and solid benzalkonium chloride preparations were purchased and subjected to the above assay procedure. As can be seen from Table 1, three different batches from the same manufacturer (A) contained roughly the same proportion of the homologues, with the C, , predominating, illustrating good batch to batch consistency.…”

Preparation of Benzalkonium Salts Differing in the Length of a Side Alkyl Chain

“…The antimicrobial activity depends on a changing length of the side n-alkyl chain. It is well known that the C 12 -homolog is most effective against yeast and fungi, the C 14 -homologue against gram-positive bacteria and C 16 -homolog against gram-negative bacteria [2]. For these reasons they are widely used as preservatives for ophthalmic, nasal and parenteral products.…”

Trace analysis of benzalkonium chloride on skin by flow injection ionspray mass spectrometry–mass spectrometry

“…Chemically, BAK is a mixture of alkylbenzyldimethylammonium ions consisting of three major homologues with straight carbon chain lengths of C 12 , C 14 and C 16 . Even though BAK chloride can be safely used as an anti-microbial agent at concentrations up to 0.1%, 1 low level BAK chloride, as a result of its potential skin and ocular irritation, has been analyzed by a variety of methods such as gas chromatography, [2][3][4] high-performance liquid chromatography, [5][6][7][8][9][10][11][12][13][14] thinlayer chromatography, 15,16 spectrophotometry, [17][18][19] chemical ionization mass spectrometry, 20 fast atom-bombardment MS 21 and thermal spray LC-MS. 22 The detection limits of these methods are typically around 1-10 mg ml 21 or higher. The only method with a detection limit of 5 ng ml 21 involves an extensive sample preparation and derivatization.…”