Determination of berberine and strychnine in medicinal plants and herbal preparations by pressurized liquid extraction with capillary zone electrophoresis

Abstract

A simple and rapid method for the determination of berberine and strychnine in medicinal plants and herbal preparations for regulatory purposes using a home-made pressurized liquid extraction (PLE) system with capillary zone electrophoresis (CZE) using ultraviolet detection at 254 nm was developed. The effects of pH, concentration of buffer, and organic modifiers in the electrophoretic separation were investigated. The buffer used for CZE contained 50 mM ammonium acetate, pH 3.1. The effect of temperature on the extraction efficiency of strychnine in medicinal plants by PLE was demonstrated. Comparable or higher extraction efficiency was achieved with PLE for strychnine in medicinal plants and berberine in herbal preparations compared to soxhlet extraction. The effect of matrix interference in medicinal plants and herbal preparations containing a number of medicinal plants samples using CZE was investigated by standard additional experiments. The reproducibility of the method using PLE with CZE was found to vary between 2.4 and 10.7% (n = 5/6) for different types of samples on different days.

Recent advances in the applications of CE to forensic sciences (2001–2004)

“…The separation voltage was 18 kV. Under these conditions a sensitivity of about 2 mg/mL was achieved for both analytes [51].…”

Determination of ginsenosides in medicinal plants and health supplements by pressurized liquid extraction (PLE) with reversed phase high performance liquid chromatography

“…The PLE system used was described in earlier work [23,24]. The stainless steel tubes used were 1/16 inch OD and 0.18 mm ID.…”

“…Applications of PLE using commercially available systems based on static extraction to medicinal plants have been reported [21]. Our laboratory reported the use of a laboratory-made dynamic PLE system for the extraction of aristolochic acids, berberine, and strychnine in medicinal plants and herbal preparations [22,23,24].…”

DEVELOPMENT AND OPTIMIZATION OF A MICROWAVE-ASSISTED EXTRACTION OF PLUMIERIDE FROM Allamanda cathartica L. FLOWERS

“…27 Several advanced extraction techniques have been developed for improvement efficiency and selectivity for extracting natural compounds from herbal medicine, including pressurized liquid extractor, microwave assisted extraction (MAE), ultrasound assisted extraction and supercritical fluid extraction. [28][29][30][31] MAE is a technique that uses microwave energy to directly heat the solvent to extract target compounds from various matrices, thus accelerating the speed of heating. The highly localized temperature can cause migrations of target compounds more selectively and more rapidly (usually in less than 30 min) with similar or better recovery than traditional extraction processes with the main advantages of reducing both extraction time and solvent consumption.…”

“…The highly localized temperature can cause migrations of target compounds more selectively and more rapidly (usually in less than 30 min) with similar or better recovery than traditional extraction processes with the main advantages of reducing both extraction time and solvent consumption. [27][28][29] Many studies have been published on the application of MAE of secondary metabolites from plants. [32][33][34] In the present study, response surface methodology (RSM) was examined for optimization of MAE process parameters (solvent, irradiation power and extraction time) to obtain maximum yield of plumieride.…”