Determination of biphenyl and eight biphenols in microbial extracts by gas chromatography and thin-layer chromatography

Hydroxylations of Biphenyl by Fungi

“…Samples (4 ml) were withdrawn from the cultures, homogenized (Polytron PT-10, Brinkmann Instruments), diluted with 3 ml 0.1 M phosphate buffer (pH 6-5) and extracted with 4 ml ethyl acetate. The extracts were spotted on silica gel G F m plates (Davis et al 1978) which were developed in benzene-acetic acid (5: 1, v/v). Spots were detected by observing quenching of 254 nm induced fluorescence and by spraying with Gibb's reagent (2% in absolute ethanol: Davis et al 1978).…”

“…The extracts were spotted on silica gel G F m plates (Davis et al 1978) which were developed in benzene-acetic acid (5: 1, v/v). Spots were detected by observing quenching of 254 nm induced fluorescence and by spraying with Gibb's reagent (2% in absolute ethanol: Davis et al 1978).…”

“…(161L1640C); t.l.c., co-t.1.c. (Davis et al 1978): silica gel, benzene-acetic acid (5: I , v/v), benzene-methanol (9 : 1, v/v), benzene-methanol-28% NH40H (36 : 6 : 1, v/v); g.c.…”

“…Sublimation of the ethyl acetate extract afforded partially purified 111, which was then subjected to futher fractionation on a column of neutral alumina (Brockman, Grade I, 50 g, 2 x 28 cm) eluted with 10% methanol in benzene. Fractions corresponding,to I11 (t.l.c., Davis et al 1978) were pooled and crystallized from Skelly B…”

“…analyses were performed on a Shimadzu GC-4BM or a Hewlett-Packard 5701A gas chromatograph equipped with dual-flame ionization detectors. Oven temperatures, gas flow rates and column packings used were those described by Davis et al (1978). Infrared (i.r.)…”