Microbial Transformations of Pergolide to Pergolide Sulfoxide and Pergolide Sulfone

Smith, Robert V.; Davis, Patrick J.; Kerr, Kathleen

doi:10.1002/jps.2600720704

Cited by 14 publications

(2 citation statements)

References 27 publications

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“…Interestingly, another unreported metabolite, pergolide sulfone, is a secondary metabolite of pergolide produced by microorganisms (Smith et al, 1983) and its increase in the urine may indicate increased rumen microbiome metabolic activity of E+ steers. Using HRM to identify novel EA, their structures and biotransformation, followed by verification with targeted approaches is fundamental to our greater understanding of EA in general and of FT.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 96%

Metabolomics of fescue toxicosis in grazing beef steers

Mote

Hill

Uppal

et al. 2017

Food and Chemical Toxicology

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Fescue toxicosis (FT) results from consumption of tall fescue (Lolium arundinaceum) infected with an endophyte (Epichloë coenophiala) that produces ergot alkaloids (EA), which are considered key etiological agents of FT. Decreased weight gains, hormonal imbalance, circulating cholesterol disruption, and decreased volatile fatty acid absorption suggest toxic (E+) fescue-induced metabolic perturbations. Employing untargeted high-resolution metabolomics (HRM) to analyze E+ grazing-induced plasma and urine metabolome changes, fescue-naïve Angus steers were placed on E+ or non-toxic (Max-Q) fescue pastures and plasma and urine were sampled before, 1, 2, 14, and 28 days after pasture assignment. Plasma and urine catecholamines and urinary EA concentrations were also measured. In E+ steers, urinary EA appeared early and peaked at 14 days. 13,090 urinary and 20,908 plasma HRM features were detected; the most significant effects were observed earlier (2 days) in the urine and later (≥14 days) in the plasma. Alongside EA metabolite detection, tryptophan and lipid metabolism disruption were among the main consequences of E+ consumption. The E+ grazing-associated metabolic pathways and signatures described herein may accelerate development of novel early FT detection and treatment strategies.

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