Determination of blood circulation times of superparamagnetic iron oxide nanoparticles by T2* relaxometry using ultrashort echo time (UTE) MRI

Scharlach, Constantin; Warmuth, Carsten; Schellenberger, Eyk

doi:10.1016/j.mri.2015.06.017

Cited by 14 publications

(13 citation statements)

References 15 publications

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“…The highest blood values were observed during the first 5 min, even though the percentage of CNCs was very low, indicating the fast elimination of CNCs from the blood circulation. This is consistent with previous findings, which suggest that nanosized inorganic particles above ~50 nm become distributed very fast in the liver and spleen [ 65 ], while smaller nanoprobes have larger blood half- lives, although they end up in the same RES organs [ 66 ]. The fast accumulation in the liver could infer that these nanomaterials may be of potential use in the diagnosis and therapy of liver and spleen diseases [ 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ].…”

Section: Discussionsupporting

confidence: 93%

Iron Oxide Colloidal Nanoclusters as Theranostic Vehicles and Their Interactions at the Cellular Level

et al. 2018

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Advances in surfactant-assisted chemical approaches have led the way for the exploitation of nanoscale inorganic particles in medical diagnosis and treatment. In this field, magnetically-driven multimodal nanotools that perform both detection and therapy, well-designed in size, shape and composition, are highly advantageous. Such a theranostic material—which entails the controlled assembly of smaller (maghemite) nanocrystals in a secondary motif that is highly dispersible in aqueous media—is discussed here. These surface functionalized, pomegranate-like ferrimagnetic nanoclusters (40–85 nm) are made of nanocrystal subunits that show a remarkable magnetic resonance imaging contrast efficiency, which is better than that of the superparamagnetic contrast agent Endorem©. Going beyond this attribute and with their demonstrated low cytotoxicity in hand, we examine the critical interaction of such nanoprobes with cells at different physiological environments. The time-dependent in vivo scintigraphic imaging of mice experimental models, combined with a biodistribution study, revealed the accumulation of nanoclusters in the spleen and liver. Moreover, the in vitro proliferation of spleen cells and cytokine production witnessed a size-selective regulation of immune system cells, inferring that smaller clusters induce mainly inflammatory activities, while larger ones induce anti-inflammatory actions. The preliminary findings corroborate that the modular chemistry of magnetic iron oxide nanoclusters stimulates unexplored pathways that could be driven to alter their function in favor of healthcare.

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Section: Discussionsupporting

confidence: 93%

Iron Oxide Colloidal Nanoclusters as Theranostic Vehicles and Their Interactions at the Cellular Level

et al. 2018

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“…45 Blood-pool half-life is dependent on several factors, including size, coating, dose, and charge. The half-lives of these nanoparticles are in the order of minutes 46 to hours. 47 The halflife dose dependence is relatively minimal, with clinical dose ranges having similar pharmacokinetic profiles.…”

Section: Iron Pharmacokineticsmentioning

confidence: 99%

“…46 This approach could be adapted to determine absolute concentration if r 2 * were known for these nanoparticles. 46 When performing angiography and interrogating the vasculature, it is sometimes of use to examine other properties of the vasculature. T 1 , T 2 , and T 2 * are complementary in this effort, with each providing the means (when coupled with a contrast agent) to quantify different vascular properties.…”

Section: Quantification With Iron Oxide Agentsmentioning

confidence: 99%

Iron Oxide as an Mri Contrast Agent for Cell Tracking: Supplementary Issue

Korchinski

Taha

Yang

et al. 2015

Magn Reson�Insights

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Iron oxide contrast agents have been combined with magnetic resonance imaging for cell tracking. In this review, we discuss coating properties and provide an overview of ex vivo and in vivo labeling of different cell types, including stem cells, red blood cells, and monocytes/macrophages. Furthermore, we provide examples of applications of cell tracking with iron contrast agents in stroke, multiple sclerosis, cancer, arteriovenous malformations, and aortic and cerebral aneurysms. Attempts at quantifying iron oxide concentrations and other vascular properties are examined. We advise on designing studies using iron contrast agents including methods for validation.

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“…Positive-contrast MRI experiments with iron oxide-labeled cells have been developed and are being investigated to address this problem but are not yet in common use. 8,26,27 There were also increased numbers of activated microglia existing in regions transplanted with the MIRB-labeled NSCs. This phenomenon was substantially more pronounced in the 50 μg case, compared to animals receiving 20 μg MIRB-labeled NSCs, and can be attributed to the observed reduced survival of the 50 μg grafts which led to the release of MIRB from dying cells.…”

mentioning

confidence: 99%

Effects of the iron oxide nanoparticle Molday ION Rhodamine B on the viability and regenerative function of neural stem cells: relevance to clinical translation

Umashankar¹,

Corenblum²,

Ray³

et al. 2016

IJN

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An essential component of developing successful neural stem cell (NSC)-based therapies involves the establishment of methodologies to noninvasively monitor grafted NSCs within brain tissues in real time. In this context, ex vivo labeling with ultrasmall superparamagnetic iron oxide (USPIO) particles has been shown to enable efficient tracking of transplanted NSCs via magnetic resonance imaging (MRI). However, whether and how USPIO labeling affects the intrinsic biology of NSCs is not thoroughly understood, and remains an active area of investigation. Here, we perform a comprehensive examination of rat NSC survival and regenerative function upon labeling with the USPIO, Molday ION Rhodamine B (MIRB), which allows for dual magnetic resonance and optical imaging. After optimization of labeling efficiency, two specific doses of MIRB (20 and 50 μg/mL) were chosen and were followed for the rest of the study. We observed that both MIRB doses supported the robust detection of NSCs, over an extended period of time in vitro and in vivo after transplantation into the striata of host rats, using MRI and post hoc fluorescence imaging. Both in culture and after neural transplantation, the higher 50 μg/mL MIRB dose significantly reduced the survival, proliferation, and differentiation rate of the NSCs. Interestingly, although the lower 20 μg/mL MIRB labeling did not produce overtly negative effects, it increased the proliferation and glial differentiation of the NSCs. Additionally, application of this dose also changed the morphological characteristics of neurons and glia produced after NSC differentiation. Importantly, the transplantation of NSCs labeled with either of the two MIRB doses upregulated the immune response in recipient animals. In particular, in animals receiving the 50 μg/mL MIRB-labeled NSCs, this immune response consisted of an increased number of CD68 + -activated microglia, which appeared to have phagocytosed MIRB particles and cells contributing to an exaggerated MRI signal dropout in the animals. Overall, these results indicate that although USPIO particles, such as MIRB, may have advantageous labeling and magnetic resonance-sensitive features for NSC tracking, a further examination of their effects might be necessary before they can be used in clinical scenarios of cell-based transplantation.

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Determination of blood circulation times of superparamagnetic iron oxide nanoparticles by T2* relaxometry using ultrashort echo time (UTE) MRI

Cited by 14 publications

References 15 publications

Iron Oxide Colloidal Nanoclusters as Theranostic Vehicles and Their Interactions at the Cellular Level

Iron Oxide Colloidal Nanoclusters as Theranostic Vehicles and Their Interactions at the Cellular Level

Iron Oxide as an Mri Contrast Agent for Cell Tracking: Supplementary Issue

Effects of the iron oxide nanoparticle Molday ION Rhodamine B on the viability and regenerative function of neural stem cells: relevance to clinical translation

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